A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla
S García-Gallego, M., & Akharbach, H. (1998). Aquaculture International, 6(5), 345-356. doi:10.1023/a:1009207904037 [EN] A multi-technique approach was used to study the changes occurring in European eel Anguilla anguilla ovaries during hormonally-induced vitellogenesis. Aside from classic techn...
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Online Access: | https://doi.org/10.1111/jfb.13103 http://hdl.handle.net/10251/98351 |
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fttriple:oai:gotriple.eu:http://hdl.handle.net/10251/98351 2023-05-15T13:27:31+02:00 A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla Mazzeo ., Ilaria Giorgini, E. Gioacchini, G. Maradonna, F. Vilchez Olivencia, Maria Carmen Baloche, S. Dufour, S. Pérez Igualada, Luz María Carnevali, O. Asturiano Nemesio, Juan Francisco Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission 2016-01-01 https://doi.org/10.1111/jfb.13103 http://hdl.handle.net/10251/98351 en eng Blackwell Publishing urn:issn:0022-1112 doi:10.1111/jfb.13103 27500665 http://hdl.handle.net/10251/98351 http://doi.org/10.1111/jfb.13103 other Repositorio Institucional de la Universitat Politècnica de València geo art Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2016 fttriple https://doi.org/10.1111/jfb.13103 2023-01-22T17:25:06Z S García-Gallego, M., & Akharbach, H. (1998). Aquaculture International, 6(5), 345-356. doi:10.1023/a:1009207904037 [EN] A multi-technique approach was used to study the changes occurring in European eel Anguilla anguilla ovaries during hormonally-induced vitellogenesis. Aside from classic techniques used to monitor the vitellogenic process, such as ovary histology, fat content analysis, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and vitellogenin enzyme linked immunosorbent assay (ELISA), a new technique, Fourier-transform infrared (FT-IR) microspectroscopy, was used to analyse A. anguilla ovaries. The results from the different techniques provided different ways of approaching the same process. Although it is considered a time consuming approach, of all the employed techniques, histology provided the most direct evidences about vitellogenesis. SDS-PAGE and ELISA were also useful for studying vitellogenesis, whereas fat analysis cannot be used for this purpose. The FT-IR analysis provided a representative IR spectrum for each ovarian stage (previtellogenic stage, early vitellogenic stage, mid-vitellogenic stage and late vitellogenic stage), demonstrating that it is a valid method able to illustrate the distribution of the oocytes within the ovary slices. The chemical maps obtained confirmed changes in lipid concentrations and revealed their distribution within the oocytes at different maturational stages. When the results and the accuracy of the FT-IR analysis were compared with those of the traditional techniques commonly used to establish the vitellogenic stage, it became evident that FT-IR is a useful and reliable tool, with many advantages, including the fact that it requires little biological material, the costs involved are low, analysis times are short and last but not least, the fact that it offers the possibility of simultaneously analysing various biocomponents of the same oocyte. (C) 2016 The Fisheries Society of the British Isles This study was funded by the ... Article in Journal/Newspaper Anguilla anguilla Unknown Journal of Fish Biology 89 4 2055 2069 |
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fttriple |
language |
English |
topic |
geo art |
spellingShingle |
geo art Mazzeo ., Ilaria Giorgini, E. Gioacchini, G. Maradonna, F. Vilchez Olivencia, Maria Carmen Baloche, S. Dufour, S. Pérez Igualada, Luz María Carnevali, O. Asturiano Nemesio, Juan Francisco A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla |
topic_facet |
geo art |
description |
S García-Gallego, M., & Akharbach, H. (1998). Aquaculture International, 6(5), 345-356. doi:10.1023/a:1009207904037 [EN] A multi-technique approach was used to study the changes occurring in European eel Anguilla anguilla ovaries during hormonally-induced vitellogenesis. Aside from classic techniques used to monitor the vitellogenic process, such as ovary histology, fat content analysis, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and vitellogenin enzyme linked immunosorbent assay (ELISA), a new technique, Fourier-transform infrared (FT-IR) microspectroscopy, was used to analyse A. anguilla ovaries. The results from the different techniques provided different ways of approaching the same process. Although it is considered a time consuming approach, of all the employed techniques, histology provided the most direct evidences about vitellogenesis. SDS-PAGE and ELISA were also useful for studying vitellogenesis, whereas fat analysis cannot be used for this purpose. The FT-IR analysis provided a representative IR spectrum for each ovarian stage (previtellogenic stage, early vitellogenic stage, mid-vitellogenic stage and late vitellogenic stage), demonstrating that it is a valid method able to illustrate the distribution of the oocytes within the ovary slices. The chemical maps obtained confirmed changes in lipid concentrations and revealed their distribution within the oocytes at different maturational stages. When the results and the accuracy of the FT-IR analysis were compared with those of the traditional techniques commonly used to establish the vitellogenic stage, it became evident that FT-IR is a useful and reliable tool, with many advantages, including the fact that it requires little biological material, the costs involved are low, analysis times are short and last but not least, the fact that it offers the possibility of simultaneously analysing various biocomponents of the same oocyte. (C) 2016 The Fisheries Society of the British Isles This study was funded by the ... |
author2 |
Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission |
format |
Article in Journal/Newspaper |
author |
Mazzeo ., Ilaria Giorgini, E. Gioacchini, G. Maradonna, F. Vilchez Olivencia, Maria Carmen Baloche, S. Dufour, S. Pérez Igualada, Luz María Carnevali, O. Asturiano Nemesio, Juan Francisco |
author_facet |
Mazzeo ., Ilaria Giorgini, E. Gioacchini, G. Maradonna, F. Vilchez Olivencia, Maria Carmen Baloche, S. Dufour, S. Pérez Igualada, Luz María Carnevali, O. Asturiano Nemesio, Juan Francisco |
author_sort |
Mazzeo ., Ilaria |
title |
A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla |
title_short |
A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla |
title_full |
A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla |
title_fullStr |
A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla |
title_full_unstemmed |
A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla |
title_sort |
comparison of techniques for studying oogenesis in the european eel anguilla anguilla |
publisher |
Blackwell Publishing |
publishDate |
2016 |
url |
https://doi.org/10.1111/jfb.13103 http://hdl.handle.net/10251/98351 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Repositorio Institucional de la Universitat Politècnica de València |
op_relation |
urn:issn:0022-1112 doi:10.1111/jfb.13103 27500665 http://hdl.handle.net/10251/98351 http://doi.org/10.1111/jfb.13103 |
op_rights |
other |
op_doi |
https://doi.org/10.1111/jfb.13103 |
container_title |
Journal of Fish Biology |
container_volume |
89 |
container_issue |
4 |
container_start_page |
2055 |
op_container_end_page |
2069 |
_version_ |
1766398873056575488 |