The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime
S [EN] There has been a marked reduction in natural stocks of eels (genus Anguilla) over the past 60 years, and the culture of eels is still based on the capture of very large quantities of juveniles. It is necessary to close the life cycle in captivity in order to ease the pressure on wild populati...
Published in: | Reproduction, Fertility and Development |
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2015
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Online Access: | https://doi.org/10.1071/RD13198 http://hdl.handle.net/10251/68522 |
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fttriple:oai:gotriple.eu:http://hdl.handle.net/10251/68522 2023-05-15T13:28:20+02:00 The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime Gallego Albiach, Victor Vilchez Olivencia, Maria Carmen Peñaranda, D.S. Pérez Igualada, Luz María Herraez, María Paz Asturiano Nemesio, Juan Francisco Martinez-Pastor, Felipe Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission Generalitat Valenciana Ministerio de Economía y Competitividad Ministerio de Ciencia e Innovación Universitat Politècnica de València 2015-01-01 https://doi.org/10.1071/RD13198 http://hdl.handle.net/10251/68522 en eng CSIRO Publishing urn:issn:1031-3613 doi:10.1071/RD13198 urn:eissn:1448-5990 http://hdl.handle.net/10251/68522 http://dx.doi.org/10.1071/RD13198 other Repositorio Institucional de la Universitat Politècnica de València envir socio Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2015 fttriple https://doi.org/10.1071/RD13198 2023-01-22T18:12:08Z S [EN] There has been a marked reduction in natural stocks of eels (genus Anguilla) over the past 60 years, and the culture of eels is still based on the capture of very large quantities of juveniles. It is necessary to close the life cycle in captivity in order to ease the pressure on wild populations. The aims of the present study were to evaluate sperm subpopulations (through cluster analysis of computer-aided sperm analysis data) in the European eel (Anguilla anguilla) and to assess the effects of motility acquisition time after activation (i.e. at 30, 60 and 90 s), the thermal regimen (i.e. 10 degrees C (T10) or 15 degrees C (T15) and up to 20 degrees C, or constant at 20 degrees C (T20)) and hormonal treatments (i.e. human chorionic gonadotropin (hCG), recombinant (r) hCG or pregnant mare serum gonadotropin (PMSG)) on these subpopulations. In all cases, we obtained three subpopulations of spermatozoa: low velocity and linear (S1); high velocity with low linearity (S2); and high velocity and linear (S3; considered high quality). Total motility and S1 were affected by acquisition time; thus, 30 s is recommended as the standard time for motility acquisition. When eels were kept at 20 degrees C (T20), motility data fitted quadratic models, with the highest motility and proportion of S3 between Weeks 8 and 12 after the first injection. Lower temperatures (T10, T15) delayed spermiation and the obtaining of high-quality spermatozoa (S3), but did not seem to alter the spermiation process (similar subpopulation pattern). Conversely, the hormonal treatments altered both the dynamics of the subpopulation pattern and the onset of spermiation (with PMSG delaying it). Total motility and the yield of S3 with the widely used hCG treatment varied throughout the spermiation period. However, using rhCG allowed us to obtain high-quality and constant motility for most of the study (Weeks 7-20), and the S3 yield was also higher overall (61.8 +/- 1.3%; mean +/- s.e.m.) and more stable over time than the other hormonal ... Article in Journal/Newspaper Anguilla anguilla Unknown Reproduction, Fertility and Development 27 3 529 |
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envir socio |
spellingShingle |
envir socio Gallego Albiach, Victor Vilchez Olivencia, Maria Carmen Peñaranda, D.S. Pérez Igualada, Luz María Herraez, María Paz Asturiano Nemesio, Juan Francisco Martinez-Pastor, Felipe The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime |
topic_facet |
envir socio |
description |
S [EN] There has been a marked reduction in natural stocks of eels (genus Anguilla) over the past 60 years, and the culture of eels is still based on the capture of very large quantities of juveniles. It is necessary to close the life cycle in captivity in order to ease the pressure on wild populations. The aims of the present study were to evaluate sperm subpopulations (through cluster analysis of computer-aided sperm analysis data) in the European eel (Anguilla anguilla) and to assess the effects of motility acquisition time after activation (i.e. at 30, 60 and 90 s), the thermal regimen (i.e. 10 degrees C (T10) or 15 degrees C (T15) and up to 20 degrees C, or constant at 20 degrees C (T20)) and hormonal treatments (i.e. human chorionic gonadotropin (hCG), recombinant (r) hCG or pregnant mare serum gonadotropin (PMSG)) on these subpopulations. In all cases, we obtained three subpopulations of spermatozoa: low velocity and linear (S1); high velocity with low linearity (S2); and high velocity and linear (S3; considered high quality). Total motility and S1 were affected by acquisition time; thus, 30 s is recommended as the standard time for motility acquisition. When eels were kept at 20 degrees C (T20), motility data fitted quadratic models, with the highest motility and proportion of S3 between Weeks 8 and 12 after the first injection. Lower temperatures (T10, T15) delayed spermiation and the obtaining of high-quality spermatozoa (S3), but did not seem to alter the spermiation process (similar subpopulation pattern). Conversely, the hormonal treatments altered both the dynamics of the subpopulation pattern and the onset of spermiation (with PMSG delaying it). Total motility and the yield of S3 with the widely used hCG treatment varied throughout the spermiation period. However, using rhCG allowed us to obtain high-quality and constant motility for most of the study (Weeks 7-20), and the S3 yield was also higher overall (61.8 +/- 1.3%; mean +/- s.e.m.) and more stable over time than the other hormonal ... |
author2 |
Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission Generalitat Valenciana Ministerio de Economía y Competitividad Ministerio de Ciencia e Innovación Universitat Politècnica de València |
format |
Article in Journal/Newspaper |
author |
Gallego Albiach, Victor Vilchez Olivencia, Maria Carmen Peñaranda, D.S. Pérez Igualada, Luz María Herraez, María Paz Asturiano Nemesio, Juan Francisco Martinez-Pastor, Felipe |
author_facet |
Gallego Albiach, Victor Vilchez Olivencia, Maria Carmen Peñaranda, D.S. Pérez Igualada, Luz María Herraez, María Paz Asturiano Nemesio, Juan Francisco Martinez-Pastor, Felipe |
author_sort |
Gallego Albiach, Victor |
title |
The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime |
title_short |
The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime |
title_full |
The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime |
title_fullStr |
The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime |
title_full_unstemmed |
The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime |
title_sort |
subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime |
publisher |
CSIRO Publishing |
publishDate |
2015 |
url |
https://doi.org/10.1071/RD13198 http://hdl.handle.net/10251/68522 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Repositorio Institucional de la Universitat Politècnica de València |
op_relation |
urn:issn:1031-3613 doi:10.1071/RD13198 urn:eissn:1448-5990 http://hdl.handle.net/10251/68522 http://dx.doi.org/10.1071/RD13198 |
op_rights |
other |
op_doi |
https://doi.org/10.1071/RD13198 |
container_title |
Reproduction, Fertility and Development |
container_volume |
27 |
container_issue |
3 |
container_start_page |
529 |
_version_ |
1766403480609619968 |