Uso de diferentes tipos de gonadotropinas (purificadas vs recombinantes) para inducir la maduración sexual en machos de anguila europea

• Main Author: Pérez Albelo, Irene
• Other Authors: Asturiano Nemesio, Juan Francisco, Gallego Albiach, Victor, Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural
• Format: Thesis
• Language: Spanish
• Published: Universitat Politècnica de València 2021
• Subjects: envir; psy; Anguilla anguilla; European eel
• Online Access: http://hdl.handle.net/10251/174090

[EN] In the past three decades the European eel (Anguilla Anguilla) experienced up to 99% decline in recruitment in some parts of its distribution range, thus breeding in captivity is nowadays considered key to save this species. With this in mind, obtaining high quality gametes is fundamental, as is the ongoing study of new hormonal treatments in order to improve current methods. Therefore, the aim of this research study is to assess the effect of two hormonal treatments (OVI, a recombinant ¿-choriogonadotropin; and VET, a human chorionic gonadotropin purified from female urine) on the reproductive performance of European eel males, and, after choosing the best hormone, to compare the effects of three different doses in order to cut the costs of artificial maturation. Results indicate that the type of hormone used (recombinant vs purified gonadotropins) significantly affects the progression of spermiation in European eel males, and that the recombinant hormone (OVI) produces better results in terms of sperm quantity and quality in most of the weeks of the treatment, remaining thus an effective treatment to induce spermiation in this species. On the other hand, in terms of the doses experiment, results show that from the lowest to the highest dose (0.25 to 1.5 IU/g fish) all the treatments are able to induce the whole spermiation process. However, a weekly dose of 1.5 IU/g fish of recombinant hormone (OVI) is necessary in order to provide a notable amount (volume and density) of high quality (motility and velocity) samples throughout the treatment. Finally, the economic analysis demonstrates that the recombinant hormone (OVI, 1.5 IU/g fish) has a greater profitability than the other treatments, making it possible to obtain highquality sperm for a lower price. In this context and considering the fact that in the first few weeks of any hormonal treatment there is no high-quality sperm production, long-term hormonal therapies are necessary in order to lessen the cost of high-quality European eel sperm. TFGM [ES] ...