Intracellular pH regulation and sperm motility in the European eel
S Fechner, S., Alvarez, L., Bönigk, W., Müller, A., Berger, T. K., Pascal, R., … Kaupp, U. B. (2015). A K+-selective CNG channel orchestrates Ca2+ signalling in zebrafish sperm. eLife, 4. doi:10.7554/elife.07624 [EN] Sperm activation involves ion fluxes as well as a previous maturation in the semina...
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fttriple:oai:gotriple.eu:http://hdl.handle.net/10251/171696 2023-05-15T16:08:38+02:00 Intracellular pH regulation and sperm motility in the European eel Pérez Igualada, Luz María Gallego Albiach, Victor Asturiano, Juan F. Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission AGENCIA ESTATAL DE INVESTIGACION 2020-03-15 https://doi.org/10.1016/j.theriogenology.2020.01.026 http://hdl.handle.net/10251/171696 en eng Elsevier urn:issn:0093-691X doi:10.1016/j.theriogenology.2020.01.026 31986301 http://hdl.handle.net/10251/171696 lic_creative-commons Repositorio Institucional de la Universitat Politècnica de València hist scipo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2020 fttriple https://doi.org/10.1016/j.theriogenology.2020.01.026 2023-01-22T18:32:59Z S Fechner, S., Alvarez, L., Bönigk, W., Müller, A., Berger, T. K., Pascal, R., … Kaupp, U. B. (2015). A K+-selective CNG channel orchestrates Ca2+ signalling in zebrafish sperm. eLife, 4. doi:10.7554/elife.07624 [EN] Sperm activation involves ion fluxes as well as a previous maturation in the seminal plasma, something which has not been studied in depth in marine fish species. pH and potassium are probably involved in sperm maturation and motility in the European eel, as indicated in previous studies. In this work, the absolute intracellular concentration of potassium in European eel sperm has been determined for the first time. In addition, the intracellular pH (pH(i)) of quiescent eel spermatozoa was determined by two methods (nigericin and null point) that gave similar results, 7.4-7.6. The natural pH(i) range of sperm samples in the quiescent stage was 7.4-8.0, with no evident relationship with sperm motility. However, a linear correlation was seen between sperm motility and the pH of the diluent or extracellular pH (pH(e)), as well as between the pH(i) and the pH of the diluent. The change post-activation in seawater (ASW) depended on the initial pH(e) of the diluent medium. Activation with ASW induced an internal alkalinization of the cells when the sample had previously been diluted in a pH(e) 8, and no pH(i) variation when pH(e) was 8.0. These experiments indicated that a careful selection of the diluents should be performed before measuring natural pH(i) changes in sperm cells. Thus, studies on the specific seminal plasma composition of marine fish species are necessary before studying their physiology. Furthermore, our study indicates that intracellular alkalinization is not a universal fact during sperm activation. (C) 2020 Elsevier Inc. All rights reserved. Funded by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 642893 (IMPRESS). Victor Gallego has a postdoc grant from the MICIU (Juan de la Cierva-Incorporacion; IJCI-2017-34200 ... Article in Journal/Newspaper European eel Unknown Alvarez ENVELOPE(-64.483,-64.483,-65.633,-65.633) Cierva ENVELOPE(-60.873,-60.873,-64.156,-64.156) Theriogenology 145 48 58 |
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English |
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hist scipo Pérez Igualada, Luz María Gallego Albiach, Victor Asturiano, Juan F. Intracellular pH regulation and sperm motility in the European eel |
topic_facet |
hist scipo |
description |
S Fechner, S., Alvarez, L., Bönigk, W., Müller, A., Berger, T. K., Pascal, R., … Kaupp, U. B. (2015). A K+-selective CNG channel orchestrates Ca2+ signalling in zebrafish sperm. eLife, 4. doi:10.7554/elife.07624 [EN] Sperm activation involves ion fluxes as well as a previous maturation in the seminal plasma, something which has not been studied in depth in marine fish species. pH and potassium are probably involved in sperm maturation and motility in the European eel, as indicated in previous studies. In this work, the absolute intracellular concentration of potassium in European eel sperm has been determined for the first time. In addition, the intracellular pH (pH(i)) of quiescent eel spermatozoa was determined by two methods (nigericin and null point) that gave similar results, 7.4-7.6. The natural pH(i) range of sperm samples in the quiescent stage was 7.4-8.0, with no evident relationship with sperm motility. However, a linear correlation was seen between sperm motility and the pH of the diluent or extracellular pH (pH(e)), as well as between the pH(i) and the pH of the diluent. The change post-activation in seawater (ASW) depended on the initial pH(e) of the diluent medium. Activation with ASW induced an internal alkalinization of the cells when the sample had previously been diluted in a pH(e) 8, and no pH(i) variation when pH(e) was 8.0. These experiments indicated that a careful selection of the diluents should be performed before measuring natural pH(i) changes in sperm cells. Thus, studies on the specific seminal plasma composition of marine fish species are necessary before studying their physiology. Furthermore, our study indicates that intracellular alkalinization is not a universal fact during sperm activation. (C) 2020 Elsevier Inc. All rights reserved. Funded by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 642893 (IMPRESS). Victor Gallego has a postdoc grant from the MICIU (Juan de la Cierva-Incorporacion; IJCI-2017-34200 ... |
author2 |
Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission AGENCIA ESTATAL DE INVESTIGACION |
format |
Article in Journal/Newspaper |
author |
Pérez Igualada, Luz María Gallego Albiach, Victor Asturiano, Juan F. |
author_facet |
Pérez Igualada, Luz María Gallego Albiach, Victor Asturiano, Juan F. |
author_sort |
Pérez Igualada, Luz María |
title |
Intracellular pH regulation and sperm motility in the European eel |
title_short |
Intracellular pH regulation and sperm motility in the European eel |
title_full |
Intracellular pH regulation and sperm motility in the European eel |
title_fullStr |
Intracellular pH regulation and sperm motility in the European eel |
title_full_unstemmed |
Intracellular pH regulation and sperm motility in the European eel |
title_sort |
intracellular ph regulation and sperm motility in the european eel |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.theriogenology.2020.01.026 http://hdl.handle.net/10251/171696 |
long_lat |
ENVELOPE(-64.483,-64.483,-65.633,-65.633) ENVELOPE(-60.873,-60.873,-64.156,-64.156) |
geographic |
Alvarez Cierva |
geographic_facet |
Alvarez Cierva |
genre |
European eel |
genre_facet |
European eel |
op_source |
Repositorio Institucional de la Universitat Politècnica de València |
op_relation |
urn:issn:0093-691X doi:10.1016/j.theriogenology.2020.01.026 31986301 http://hdl.handle.net/10251/171696 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.1016/j.theriogenology.2020.01.026 |
container_title |
Theriogenology |
container_volume |
145 |
container_start_page |
48 |
op_container_end_page |
58 |
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1766404663054172160 |