Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii)
S [EN] It is essential to define an optimized standard method to assess the fish sperm quality to minimize the differences between the results obtained by different laboratories. Only this optimization and standardization can make them useful from academia to industry. This study presents the valida...
Published in: | Aquaculture |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://doi.org/10.1016/j.aquaculture.2018.12.001 http://hdl.handle.net/10251/120000 |
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fttriple:oai:gotriple.eu:http://hdl.handle.net/10251/120000 2023-05-15T13:01:47+02:00 Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii) Caldeira, Carina Hernández-Ibáñez, Sandra Valverde, Anthony Martin, Patrick Herranz-Jusdado, Juan Germán Gallego Albiach, Victor Asturiano Nemesio, Juan Francisco Dzyuba, Borys Psenicka, Martin Soler, Carles Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission 2019-01-01 https://doi.org/10.1016/j.aquaculture.2018.12.001 http://hdl.handle.net/10251/120000 en eng Elsevier urn:issn:0044-8486 doi:10.1016/j.aquaculture.2018.12.001 http://hdl.handle.net/10251/120000 lic_creative-commons other Repositorio Institucional de la Universitat Politècnica de València anthro-se envir Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2019 fttriple https://doi.org/10.1016/j.aquaculture.2018.12.001 2023-01-22T18:37:28Z S [EN] It is essential to define an optimized standard method to assess the fish sperm quality to minimize the differences between the results obtained by different laboratories. Only this optimization and standardization can make them useful from academia to industry. This study presents the validation of sperm motility assessment using a CASA-Mot system for three endangered diadromous fish species: European eel (Anguilla anguilla), Atlantic salmon (Salmo salar) and Siberian sturgeon (Acipenser baerii). To attain this goal, different technical and data processing methods were tested: 1) magnification lens (×10 and ×20), 2) Spermtrack® reusable chambers (10 and 20¿¿m depth) and 3) different frame rates (50¿¿¿FR¿¿¿250). The results suggested that the sperm motility assessment for eel, salmon and sturgeon should be performed at 200, 250 and 225¿frames¿s¿1, respectively. Moreover, to obtain a high number of analysed spermatozoa in less time and a natural movement of the sperm cells, it is recommended to use ×10 objective and 20¿¿m depth. In conclusion, different technical settings influence sperm kinetic parameters and should be validated for each fish species to allow the comparison of results between laboratories. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No [642893]. AV is granted by the CONICIT and MICITT, Costa Rica. The study was financially supported by the Ministry of Education, Youth and Sports of the Czech Republic projects "CENAKVA" (No. CZ.1.05/2.1.00/01.0024), "CENAKVA II" (No. LO1205 under the NPU I program), project Biodiversity (CZ.02.1.01/0.0/0.0/16_025/0007370), by the Czech Science Foundation (project No. 17-19714Y). Caldeira, C.; Hernández-Ibáñez, S.; Valverde, A.; Martin, P.; Herranz-Jusdado, JG.; Gallego Albiach, V.; Asturiano Nemesio, JF. (2019). Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and ... Article in Journal/Newspaper Acipenser baerii Anguilla anguilla Atlantic salmon European eel Salmo salar Siberian sturgeon Unknown Nemesio ENVELOPE(-59.467,-59.467,-65.267,-65.267) Aquaculture 502 223 231 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
anthro-se envir |
spellingShingle |
anthro-se envir Caldeira, Carina Hernández-Ibáñez, Sandra Valverde, Anthony Martin, Patrick Herranz-Jusdado, Juan Germán Gallego Albiach, Victor Asturiano Nemesio, Juan Francisco Dzyuba, Borys Psenicka, Martin Soler, Carles Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii) |
topic_facet |
anthro-se envir |
description |
S [EN] It is essential to define an optimized standard method to assess the fish sperm quality to minimize the differences between the results obtained by different laboratories. Only this optimization and standardization can make them useful from academia to industry. This study presents the validation of sperm motility assessment using a CASA-Mot system for three endangered diadromous fish species: European eel (Anguilla anguilla), Atlantic salmon (Salmo salar) and Siberian sturgeon (Acipenser baerii). To attain this goal, different technical and data processing methods were tested: 1) magnification lens (×10 and ×20), 2) Spermtrack® reusable chambers (10 and 20¿¿m depth) and 3) different frame rates (50¿¿¿FR¿¿¿250). The results suggested that the sperm motility assessment for eel, salmon and sturgeon should be performed at 200, 250 and 225¿frames¿s¿1, respectively. Moreover, to obtain a high number of analysed spermatozoa in less time and a natural movement of the sperm cells, it is recommended to use ×10 objective and 20¿¿m depth. In conclusion, different technical settings influence sperm kinetic parameters and should be validated for each fish species to allow the comparison of results between laboratories. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No [642893]. AV is granted by the CONICIT and MICITT, Costa Rica. The study was financially supported by the Ministry of Education, Youth and Sports of the Czech Republic projects "CENAKVA" (No. CZ.1.05/2.1.00/01.0024), "CENAKVA II" (No. LO1205 under the NPU I program), project Biodiversity (CZ.02.1.01/0.0/0.0/16_025/0007370), by the Czech Science Foundation (project No. 17-19714Y). Caldeira, C.; Hernández-Ibáñez, S.; Valverde, A.; Martin, P.; Herranz-Jusdado, JG.; Gallego Albiach, V.; Asturiano Nemesio, JF. (2019). Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and ... |
author2 |
Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal European Commission |
format |
Article in Journal/Newspaper |
author |
Caldeira, Carina Hernández-Ibáñez, Sandra Valverde, Anthony Martin, Patrick Herranz-Jusdado, Juan Germán Gallego Albiach, Victor Asturiano Nemesio, Juan Francisco Dzyuba, Borys Psenicka, Martin Soler, Carles |
author_facet |
Caldeira, Carina Hernández-Ibáñez, Sandra Valverde, Anthony Martin, Patrick Herranz-Jusdado, Juan Germán Gallego Albiach, Victor Asturiano Nemesio, Juan Francisco Dzyuba, Borys Psenicka, Martin Soler, Carles |
author_sort |
Caldeira, Carina |
title |
Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii) |
title_short |
Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii) |
title_full |
Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii) |
title_fullStr |
Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii) |
title_full_unstemmed |
Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii) |
title_sort |
standardization of sperm motility analysis by using casa-mot for atlantic salmon (salmo salar), european eel (anguilla anguilla) and siberian sturgeon (acipenser baerii) |
publisher |
Elsevier |
publishDate |
2019 |
url |
https://doi.org/10.1016/j.aquaculture.2018.12.001 http://hdl.handle.net/10251/120000 |
long_lat |
ENVELOPE(-59.467,-59.467,-65.267,-65.267) |
geographic |
Nemesio |
geographic_facet |
Nemesio |
genre |
Acipenser baerii Anguilla anguilla Atlantic salmon European eel Salmo salar Siberian sturgeon |
genre_facet |
Acipenser baerii Anguilla anguilla Atlantic salmon European eel Salmo salar Siberian sturgeon |
op_source |
Repositorio Institucional de la Universitat Politècnica de València |
op_relation |
urn:issn:0044-8486 doi:10.1016/j.aquaculture.2018.12.001 http://hdl.handle.net/10251/120000 |
op_rights |
lic_creative-commons other |
op_doi |
https://doi.org/10.1016/j.aquaculture.2018.12.001 |
container_title |
Aquaculture |
container_volume |
502 |
container_start_page |
223 |
op_container_end_page |
231 |
_version_ |
1766286844754919424 |