Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms
Research in evolutionary biology involving nonmodel organisms is rapidly shifting from using traditional molecular markers such as mtDNA and microsatellites to higher throughput SNP genotyping methodologies to address questions in population genetics, phylogenetics and genetic mapping. Restriction s...
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Online Access: | https://doi.org/10.5061/dryad.7tb72 https://nbn-resolving.org/urn:nbn:nl:ui:13-49-mvs6 |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::eeb34dcef4d003bc673542294273b136 2023-05-15T14:30:13+02:00 Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms Recknagel, Hans Jacobs, Arne Herzyk, Pawel Elmer, Kathryn R. 2015-03-19 https://doi.org/10.5061/dryad.7tb72 https://nbn-resolving.org/urn:nbn:nl:ui:13-49-mvs6 en eng Dryad http://dx.doi.org/10.5061/dryad.7tb72 https://dx.doi.org/10.5061/dryad.7tb72 http://dx.doi.org/https://doi.org/10.5061/dryad.7tb72 https://nbn-resolving.org/urn:nbn:nl:ui:13-49-mvs6 lic_creative-commons 10.5061/dryad.7tb72 oai:easy.dans.knaw.nl:easy-dataset:88494 93989dc5-f981-4864-8edc-405e93f0a4f0 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:88494 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|re3data_____::730f562f9efe8a3b3742d2da510d4335 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 re3data_____::r3d100000044 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c Genomics/Proteomics Natural Selection and Contemporary Evolution phylogeography molecular evolution Ecological Genetics Life sciences medicine and health care Medicine quantitative genetics info socio Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2015 fttriple https://doi.org/10.5061/dryad.7tb72 2023-01-22T16:52:02Z Research in evolutionary biology involving nonmodel organisms is rapidly shifting from using traditional molecular markers such as mtDNA and microsatellites to higher throughput SNP genotyping methodologies to address questions in population genetics, phylogenetics and genetic mapping. Restriction site associated DNA sequencing (RAD sequencing or RADseq) has become an established method for SNP genotyping on Illumina sequencing platforms. Here, we developed a protocol and adapters for double-digest RAD sequencing for Ion Torrent (Life Technologies; Ion Proton, Ion PGM) semiconductor sequencing. We sequenced thirteen genomic libraries of three different nonmodel vertebrate species on Ion Proton with PI chips: Arctic charr Salvelinus alpinus, European whitefish Coregonus lavaretus and common lizard Zootoca vivipara. This resulted in ~962 million single-end reads overall and a mean of ~74 million reads per library. We filtered the genomic data using Stacks, a bioinformatic tool to process RAD sequencing data. On average, we obtained ~11 000 polymorphic loci per library of 6–30 individuals. We validate our new method by technical and biological replication, by reconstructing phylogenetic relationships, and using a hybrid genetic cross to track genomic variants. Finally, we discuss the differences between using the different sequencing platforms in the context of RAD sequencing, assessing possible advantages and disadvantages. We show that our protocol can be used for Ion semiconductor sequencing platforms for the rapid and cost-effective generation of variable and reproducible genetic markers. Table_1Table_1 contains the locus sequences and coded genotypes of each individual used to recreate genomic composition of the Alpine x Baltic cross and their offspring. SNPs that were fixed between parents (following standard genetic mapping procedure) were used in the Structure analysis. Each individual contains two columns per locus with their genotype. Numbers refer to the four different DNA bases: A = 1, C = 2, G = 3, T = ... Dataset Arctic charr Arctic Salvelinus alpinus Unknown Arctic |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
Genomics/Proteomics Natural Selection and Contemporary Evolution phylogeography molecular evolution Ecological Genetics Life sciences medicine and health care Medicine quantitative genetics info socio |
spellingShingle |
Genomics/Proteomics Natural Selection and Contemporary Evolution phylogeography molecular evolution Ecological Genetics Life sciences medicine and health care Medicine quantitative genetics info socio Recknagel, Hans Jacobs, Arne Herzyk, Pawel Elmer, Kathryn R. Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms |
topic_facet |
Genomics/Proteomics Natural Selection and Contemporary Evolution phylogeography molecular evolution Ecological Genetics Life sciences medicine and health care Medicine quantitative genetics info socio |
description |
Research in evolutionary biology involving nonmodel organisms is rapidly shifting from using traditional molecular markers such as mtDNA and microsatellites to higher throughput SNP genotyping methodologies to address questions in population genetics, phylogenetics and genetic mapping. Restriction site associated DNA sequencing (RAD sequencing or RADseq) has become an established method for SNP genotyping on Illumina sequencing platforms. Here, we developed a protocol and adapters for double-digest RAD sequencing for Ion Torrent (Life Technologies; Ion Proton, Ion PGM) semiconductor sequencing. We sequenced thirteen genomic libraries of three different nonmodel vertebrate species on Ion Proton with PI chips: Arctic charr Salvelinus alpinus, European whitefish Coregonus lavaretus and common lizard Zootoca vivipara. This resulted in ~962 million single-end reads overall and a mean of ~74 million reads per library. We filtered the genomic data using Stacks, a bioinformatic tool to process RAD sequencing data. On average, we obtained ~11 000 polymorphic loci per library of 6–30 individuals. We validate our new method by technical and biological replication, by reconstructing phylogenetic relationships, and using a hybrid genetic cross to track genomic variants. Finally, we discuss the differences between using the different sequencing platforms in the context of RAD sequencing, assessing possible advantages and disadvantages. We show that our protocol can be used for Ion semiconductor sequencing platforms for the rapid and cost-effective generation of variable and reproducible genetic markers. Table_1Table_1 contains the locus sequences and coded genotypes of each individual used to recreate genomic composition of the Alpine x Baltic cross and their offspring. SNPs that were fixed between parents (following standard genetic mapping procedure) were used in the Structure analysis. Each individual contains two columns per locus with their genotype. Numbers refer to the four different DNA bases: A = 1, C = 2, G = 3, T = ... |
format |
Dataset |
author |
Recknagel, Hans Jacobs, Arne Herzyk, Pawel Elmer, Kathryn R. |
author_facet |
Recknagel, Hans Jacobs, Arne Herzyk, Pawel Elmer, Kathryn R. |
author_sort |
Recknagel, Hans |
title |
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms |
title_short |
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms |
title_full |
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms |
title_fullStr |
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms |
title_full_unstemmed |
Data from: Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms |
title_sort |
data from: double-digest rad sequencing using ion proton semiconductor platform (ddradseq-ion) with non-model organisms |
publisher |
Dryad |
publishDate |
2015 |
url |
https://doi.org/10.5061/dryad.7tb72 https://nbn-resolving.org/urn:nbn:nl:ui:13-49-mvs6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
10.5061/dryad.7tb72 oai:easy.dans.knaw.nl:easy-dataset:88494 93989dc5-f981-4864-8edc-405e93f0a4f0 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:88494 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|re3data_____::730f562f9efe8a3b3742d2da510d4335 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 re3data_____::r3d100000044 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
http://dx.doi.org/10.5061/dryad.7tb72 https://dx.doi.org/10.5061/dryad.7tb72 http://dx.doi.org/https://doi.org/10.5061/dryad.7tb72 https://nbn-resolving.org/urn:nbn:nl:ui:13-49-mvs6 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.7tb72 |
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1766304100270473216 |