Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress
Understanding physiological responses of organisms to warming and ocean acidification is the first step towards predicting the potential population- and community-level ecological impacts of these stressors. Increasingly, physiological plasticity is being recognized as important for organisms to ada...
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fttriple:oai:gotriple.eu:50|dedup_wf_001::e524475b782ca09c411e553978684654 2023-05-15T17:50:21+02:00 Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress Wang, Jie Russell, Bayden D. Ding, Meng-Wen Dong, Yun-Wei 2019-05-08 https://doi.org/10.5061/dryad.7s3m38n undefined unknown http://dx.doi.org/10.5061/dryad.7s3m38n https://dx.doi.org/10.5061/dryad.7s3m38n lic_creative-commons oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:104033 oai:easy.dans.knaw.nl:easy-dataset:104033 10.5061/dryad.7s3m38n 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f Life sciences medicine and health care Cellana toreuma (:tba) envir anthro-bio Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2019 fttriple https://doi.org/10.5061/dryad.7s3m38n 2023-01-22T17:23:02Z Understanding physiological responses of organisms to warming and ocean acidification is the first step towards predicting the potential population- and community-level ecological impacts of these stressors. Increasingly, physiological plasticity is being recognized as important for organisms to adapt to the changing microclimates. Here, we evaluate the importance of physiological plasticity for coping with ocean acidification and elevated temperature, and its variability among individuals, of the intertidal limpet Cellana toreuma from the same population in Xiamen. Limpets were collected from shaded mid-intertidal rock surfaces. They were acclimated under combinations of different pCO2 concentrations (400 and 1000 ppm, corresponding to a pH of 8.1 and 7.8) and temperatures (20 and 24 °C) in a short-term period (7 days), with the control conditions (20 °C and 400 ppm) representing the average annual temperature and present-day pCO2 level at the collection site. Heart rates (as a proxy for metabolic performance) and expression of genes encoding inducible and constitutive heat-shock proteins (hsp70 and hsc70) at different heat-shock temperatures (26, 30, 34, and 38 °C) were measured. Hsp70 and Hsc70 play important roles in protecting cells from heat stresses, but have different expression patterns, with Hsp70 significantly increased in expression during stress and Hsc70 constitutively expressed and only mildly induced during stress. Analysis of heart rate showed significantly higher temperature coefficients (Q10 rates) for limpets at 20 °C than at 24 °C and post-acclimation thermal sensitivity of limpets at 400 ppm was lower than at 1000 ppm. Expression of hsp70 linearly increased with the increasing heat-shock temperatures, with the largest slope occurring in limpets acclimated under a future scenario (24 °C and 1000 ppm pCO2). These results suggested that limpets showed increased sensitivity and stress response under future conditions. Furthermore, the increased variation in physiological response under the ... Dataset Ocean acidification Unknown |
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Open Polar |
collection |
Unknown |
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fttriple |
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unknown |
topic |
Life sciences medicine and health care Cellana toreuma (:tba) envir anthro-bio |
spellingShingle |
Life sciences medicine and health care Cellana toreuma (:tba) envir anthro-bio Wang, Jie Russell, Bayden D. Ding, Meng-Wen Dong, Yun-Wei Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
topic_facet |
Life sciences medicine and health care Cellana toreuma (:tba) envir anthro-bio |
description |
Understanding physiological responses of organisms to warming and ocean acidification is the first step towards predicting the potential population- and community-level ecological impacts of these stressors. Increasingly, physiological plasticity is being recognized as important for organisms to adapt to the changing microclimates. Here, we evaluate the importance of physiological plasticity for coping with ocean acidification and elevated temperature, and its variability among individuals, of the intertidal limpet Cellana toreuma from the same population in Xiamen. Limpets were collected from shaded mid-intertidal rock surfaces. They were acclimated under combinations of different pCO2 concentrations (400 and 1000 ppm, corresponding to a pH of 8.1 and 7.8) and temperatures (20 and 24 °C) in a short-term period (7 days), with the control conditions (20 °C and 400 ppm) representing the average annual temperature and present-day pCO2 level at the collection site. Heart rates (as a proxy for metabolic performance) and expression of genes encoding inducible and constitutive heat-shock proteins (hsp70 and hsc70) at different heat-shock temperatures (26, 30, 34, and 38 °C) were measured. Hsp70 and Hsc70 play important roles in protecting cells from heat stresses, but have different expression patterns, with Hsp70 significantly increased in expression during stress and Hsc70 constitutively expressed and only mildly induced during stress. Analysis of heart rate showed significantly higher temperature coefficients (Q10 rates) for limpets at 20 °C than at 24 °C and post-acclimation thermal sensitivity of limpets at 400 ppm was lower than at 1000 ppm. Expression of hsp70 linearly increased with the increasing heat-shock temperatures, with the largest slope occurring in limpets acclimated under a future scenario (24 °C and 1000 ppm pCO2). These results suggested that limpets showed increased sensitivity and stress response under future conditions. Furthermore, the increased variation in physiological response under the ... |
format |
Dataset |
author |
Wang, Jie Russell, Bayden D. Ding, Meng-Wen Dong, Yun-Wei |
author_facet |
Wang, Jie Russell, Bayden D. Ding, Meng-Wen Dong, Yun-Wei |
author_sort |
Wang, Jie |
title |
Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_short |
Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_full |
Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_fullStr |
Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_full_unstemmed |
Data from: Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_sort |
data from: ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
publishDate |
2019 |
url |
https://doi.org/10.5061/dryad.7s3m38n |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:104033 oai:easy.dans.knaw.nl:easy-dataset:104033 10.5061/dryad.7s3m38n 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f |
op_relation |
http://dx.doi.org/10.5061/dryad.7s3m38n https://dx.doi.org/10.5061/dryad.7s3m38n |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.7s3m38n |
_version_ |
1766157072111501312 |