Data from: Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass

• Main Authors: Jueterbock, Alexander, Franssen, Susanne U., Bergmann, Nina, Gu, Jenny, Coyer, James A., Reusch, Thorsten B. H., Bornberg-Bauer, Erich, Olsen, Jeanine L., Jueterbock, A., Franssen, S. U., Gu, J., Bornberg-Bauer, E., Bergmann, N., Coyer, J. A., Olsen, J. L.
• Format: Dataset
• Language: unknown
• Published: Dryad Digital Repository 2016
• Subjects: RNAseq; Heatwave; Global warming; Transcriptomics; Common-garden experiment; Differential expression; North Atlantic; Zostera marina; Life sciences; medicine and health care; info; envir
• Online Access: https://doi.org/10.5061/dryad.vf5fk

Populations distributed across a broad thermal cline are instrumental in addressing adaptation to increasing temperatures under global warming. Using a space-for-time substitution design, we tested for parallel adaptation to warm temperatures along two independent thermal clines in Zostera marina, the most widely distributed seagrass in the temperate Northern Hemisphere. A North–South pair of populations was sampled along the European and North American coasts and exposed to a simulated heatwave in a common-garden mesocosm. Transcriptomic responses under control, heat stress and recovery were recorded in 99 RNAseq libraries with ~13 000 uniquely annotated, expressed genes. We corrected for phylogenetic differentiation among populations to discriminate neutral from adaptive differentiation. The two southern populations recovered faster from heat stress and showed parallel transcriptomic differentiation, as compared with northern populations. Among 2389 differentially expressed genes, 21 exceeded neutral expectations and were likely involved in parallel adaptation to warm temperatures. However, the strongest differentiation following phylogenetic correction was between the three Atlantic populations and the Mediterranean population with 128 of 4711 differentially expressed genes exceeding neutral expectations. Although adaptation to warm temperatures is expected to reduce sensitivity to heatwaves, the continued resistance of seagrass to further anthropogenic stresses may be impaired by heat-induced downregulation of genes related to photosynthesis, pathogen defence and stress tolerance. Table S1 (Excel): cDNA library characteristics of all 108 cDNA libraries.Sample preparation failed for eight libraries (indicated in the second column).stab1.xlsxTable S2 (Excel): Numbers of mapped reads.Number of reads that mapped to each of 20554 exons (rows), listed separately for each library (columns E-CX); ZosmaID (column A): gene ID based on the Zostera marina genome annotation v2.1 (GenBank Accession: LFYR00000000); mRNAID ...