Data from: Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass
Populations distributed across a broad thermal cline are instrumental in addressing adaptation to increasing temperatures under global warming. Using a space-for-time substitution design, we tested for parallel adaptation to warm temperatures along two independent thermal clines in Zostera marina, t...
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2016
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Online Access: | https://doi.org/10.5061/dryad.vf5fk |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::dabe7d9020cfe5ccc890a42d012b8f8c 2023-05-15T17:36:26+02:00 Data from: Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass Jueterbock, Alexander Franssen, Susanne U. Bergmann, Nina Gu, Jenny Coyer, James A. Reusch, Thorsten B. H. Bornberg-Bauer, Erich Olsen, Jeanine L. Jueterbock, A. Franssen, S. U. Gu, J. Bornberg-Bauer, E. Bergmann, N. Coyer, J. A. Olsen, J. L. 2016-08-29 https://doi.org/10.5061/dryad.vf5fk undefined unknown Dryad Digital Repository https://dx.doi.org/10.5061/dryad.vf5fk http://dx.doi.org/10.5061/dryad.vf5fk lic_creative-commons 10.5061/dryad.vf5fk oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:90347 oai:easy.dans.knaw.nl:easy-dataset:90347 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c RNAseq Heatwave Global warming Transcriptomics Common-garden experiment Differential expression North Atlantic Zostera marina Life sciences medicine and health care info envir Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2016 fttriple https://doi.org/10.5061/dryad.vf5fk 2023-01-22T16:53:39Z Populations distributed across a broad thermal cline are instrumental in addressing adaptation to increasing temperatures under global warming. Using a space-for-time substitution design, we tested for parallel adaptation to warm temperatures along two independent thermal clines in Zostera marina, the most widely distributed seagrass in the temperate Northern Hemisphere. A North–South pair of populations was sampled along the European and North American coasts and exposed to a simulated heatwave in a common-garden mesocosm. Transcriptomic responses under control, heat stress and recovery were recorded in 99 RNAseq libraries with ~13 000 uniquely annotated, expressed genes. We corrected for phylogenetic differentiation among populations to discriminate neutral from adaptive differentiation. The two southern populations recovered faster from heat stress and showed parallel transcriptomic differentiation, as compared with northern populations. Among 2389 differentially expressed genes, 21 exceeded neutral expectations and were likely involved in parallel adaptation to warm temperatures. However, the strongest differentiation following phylogenetic correction was between the three Atlantic populations and the Mediterranean population with 128 of 4711 differentially expressed genes exceeding neutral expectations. Although adaptation to warm temperatures is expected to reduce sensitivity to heatwaves, the continued resistance of seagrass to further anthropogenic stresses may be impaired by heat-induced downregulation of genes related to photosynthesis, pathogen defence and stress tolerance. Table S1 (Excel): cDNA library characteristics of all 108 cDNA libraries.Sample preparation failed for eight libraries (indicated in the second column).stab1.xlsxTable S2 (Excel): Numbers of mapped reads.Number of reads that mapped to each of 20554 exons (rows), listed separately for each library (columns E-CX); ZosmaID (column A): gene ID based on the Zostera marina genome annotation v2.1 (GenBank Accession: LFYR00000000); mRNAID ... Dataset North Atlantic Unknown |
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RNAseq Heatwave Global warming Transcriptomics Common-garden experiment Differential expression North Atlantic Zostera marina Life sciences medicine and health care info envir |
spellingShingle |
RNAseq Heatwave Global warming Transcriptomics Common-garden experiment Differential expression North Atlantic Zostera marina Life sciences medicine and health care info envir Jueterbock, Alexander Franssen, Susanne U. Bergmann, Nina Gu, Jenny Coyer, James A. Reusch, Thorsten B. H. Bornberg-Bauer, Erich Olsen, Jeanine L. Jueterbock, A. Franssen, S. U. Gu, J. Bornberg-Bauer, E. Bergmann, N. Coyer, J. A. Olsen, J. L. Data from: Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass |
topic_facet |
RNAseq Heatwave Global warming Transcriptomics Common-garden experiment Differential expression North Atlantic Zostera marina Life sciences medicine and health care info envir |
description |
Populations distributed across a broad thermal cline are instrumental in addressing adaptation to increasing temperatures under global warming. Using a space-for-time substitution design, we tested for parallel adaptation to warm temperatures along two independent thermal clines in Zostera marina, the most widely distributed seagrass in the temperate Northern Hemisphere. A North–South pair of populations was sampled along the European and North American coasts and exposed to a simulated heatwave in a common-garden mesocosm. Transcriptomic responses under control, heat stress and recovery were recorded in 99 RNAseq libraries with ~13 000 uniquely annotated, expressed genes. We corrected for phylogenetic differentiation among populations to discriminate neutral from adaptive differentiation. The two southern populations recovered faster from heat stress and showed parallel transcriptomic differentiation, as compared with northern populations. Among 2389 differentially expressed genes, 21 exceeded neutral expectations and were likely involved in parallel adaptation to warm temperatures. However, the strongest differentiation following phylogenetic correction was between the three Atlantic populations and the Mediterranean population with 128 of 4711 differentially expressed genes exceeding neutral expectations. Although adaptation to warm temperatures is expected to reduce sensitivity to heatwaves, the continued resistance of seagrass to further anthropogenic stresses may be impaired by heat-induced downregulation of genes related to photosynthesis, pathogen defence and stress tolerance. Table S1 (Excel): cDNA library characteristics of all 108 cDNA libraries.Sample preparation failed for eight libraries (indicated in the second column).stab1.xlsxTable S2 (Excel): Numbers of mapped reads.Number of reads that mapped to each of 20554 exons (rows), listed separately for each library (columns E-CX); ZosmaID (column A): gene ID based on the Zostera marina genome annotation v2.1 (GenBank Accession: LFYR00000000); mRNAID ... |
format |
Dataset |
author |
Jueterbock, Alexander Franssen, Susanne U. Bergmann, Nina Gu, Jenny Coyer, James A. Reusch, Thorsten B. H. Bornberg-Bauer, Erich Olsen, Jeanine L. Jueterbock, A. Franssen, S. U. Gu, J. Bornberg-Bauer, E. Bergmann, N. Coyer, J. A. Olsen, J. L. |
author_facet |
Jueterbock, Alexander Franssen, Susanne U. Bergmann, Nina Gu, Jenny Coyer, James A. Reusch, Thorsten B. H. Bornberg-Bauer, Erich Olsen, Jeanine L. Jueterbock, A. Franssen, S. U. Gu, J. Bornberg-Bauer, E. Bergmann, N. Coyer, J. A. Olsen, J. L. |
author_sort |
Jueterbock, Alexander |
title |
Data from: Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass |
title_short |
Data from: Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass |
title_full |
Data from: Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass |
title_fullStr |
Data from: Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass |
title_full_unstemmed |
Data from: Phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in Zostera marina, a globally important seagrass |
title_sort |
data from: phylogeographic differentiation versus transcriptomic adaptation to warm temperatures in zostera marina, a globally important seagrass |
publisher |
Dryad Digital Repository |
publishDate |
2016 |
url |
https://doi.org/10.5061/dryad.vf5fk |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
10.5061/dryad.vf5fk oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:90347 oai:easy.dans.knaw.nl:easy-dataset:90347 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
https://dx.doi.org/10.5061/dryad.vf5fk http://dx.doi.org/10.5061/dryad.vf5fk |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.vf5fk |
_version_ |
1766135907055828992 |