10.5061/dryad.bc276
Panagrolaimus sp. DAW1 is a freeze-tolerant Antarctic nematode which survives extensive intracellular ice formation. The molecular mechanisms of this extreme adaptation are still poorly understood. We recently showed that desiccation-enhanced RNA interference (RNAi) soaking can be used in conjunctio...
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Online Access: | https://doi.org/10.5061/dryad.bc276 |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::c66678ae29392307ea367f29b1cc31f6 2023-05-15T13:43:31+02:00 10.5061/dryad.bc276 Seybold, A.C. Wharton, David A. Thorne, Michael A.S. Marshall, Craig J. 2019-07-15 https://doi.org/10.5061/dryad.bc276 undefined unknown Europe PMC https://dx.doi.org/10.5061/dryad.bc276 http://dx.doi.org/10.5061/dryad.bc276 lic_creative-commons 10.5061/dryad.bc276 oai:easy.dans.knaw.nl:easy-dataset:99865 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:99865 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c re3data_____::r3d100000044 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 freezing tolerance Panagrolaimus davidi trehalose Cryoprotective dehydration Tps-2 Panagrolaimus DAW1 McMurdo Sound Ross Island Antarctica Life sciences medicine and health care geo envir Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2019 fttriple https://doi.org/10.5061/dryad.bc276 2023-01-22T16:51:54Z Panagrolaimus sp. DAW1 is a freeze-tolerant Antarctic nematode which survives extensive intracellular ice formation. The molecular mechanisms of this extreme adaptation are still poorly understood. We recently showed that desiccation-enhanced RNA interference (RNAi) soaking can be used in conjunction with quantitative polymerase chain reaction (qPCR) to screen for phenotypes associated with reduced expression of candidate genes in Panagrolaimus sp. DAW1. Here, we present the use of this approach to investigate the role of trehalose synthesis genes in this remarkable organism. Previous studies have shown that acclimating Panagrolaimus sp. DAW1 at 5°C before freezing or desiccation substantially enhances survival. In this study, the expression of tps-2 and other genes associated with trehalose metabolism, as well as lea-1, hsp-70 and gpx-1, in cold-acclimated and non-acclimated nematodes was analyzed using qPCR. Pd-tps-2 and Pd-lea-1 were significantly upregulated after cold acclimation, indicating an inducible expression in the cold adaptation of Panagrolaimus sp. DAW1. The role of trehalose synthesis genes in Panagrolaimus sp. DAW1 was further investigated by RNAi. Compared to the controls, Pd-tps-2a(RNAi)-treated and cold-acclimated nematodes showed a significant decrease in mRNA, but no change in trehalose content or freezing survival. The involvement of two other trehalose synthesis genes (tps-2b and gob-1) was also investigated. These findings provide the first functional genomic investigation of trehalose synthesis genes in the non-model organism Panagrolaimus sp. DAW1. The presence of several trehalose synthesis genes with different RNAi sensitivities suggests the existence of multiple backup systems in Panagrolaimus sp. DAW1, underlining the importance of this sugar in preparation for freezing. Spreadsheet containing expression dataThis file contains relative expression data for qPCR experiments both before and after RNAi, as well as freezing tolerance and trehalose content.AnnaTrehalose.xlsx Dataset Antarc* Antarctic Antarctica McMurdo Sound Ross Island Unknown Antarctic McMurdo Sound Ross Island |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
freezing tolerance Panagrolaimus davidi trehalose Cryoprotective dehydration Tps-2 Panagrolaimus DAW1 McMurdo Sound Ross Island Antarctica Life sciences medicine and health care geo envir |
spellingShingle |
freezing tolerance Panagrolaimus davidi trehalose Cryoprotective dehydration Tps-2 Panagrolaimus DAW1 McMurdo Sound Ross Island Antarctica Life sciences medicine and health care geo envir Seybold, A.C. Wharton, David A. Thorne, Michael A.S. Marshall, Craig J. 10.5061/dryad.bc276 |
topic_facet |
freezing tolerance Panagrolaimus davidi trehalose Cryoprotective dehydration Tps-2 Panagrolaimus DAW1 McMurdo Sound Ross Island Antarctica Life sciences medicine and health care geo envir |
description |
Panagrolaimus sp. DAW1 is a freeze-tolerant Antarctic nematode which survives extensive intracellular ice formation. The molecular mechanisms of this extreme adaptation are still poorly understood. We recently showed that desiccation-enhanced RNA interference (RNAi) soaking can be used in conjunction with quantitative polymerase chain reaction (qPCR) to screen for phenotypes associated with reduced expression of candidate genes in Panagrolaimus sp. DAW1. Here, we present the use of this approach to investigate the role of trehalose synthesis genes in this remarkable organism. Previous studies have shown that acclimating Panagrolaimus sp. DAW1 at 5°C before freezing or desiccation substantially enhances survival. In this study, the expression of tps-2 and other genes associated with trehalose metabolism, as well as lea-1, hsp-70 and gpx-1, in cold-acclimated and non-acclimated nematodes was analyzed using qPCR. Pd-tps-2 and Pd-lea-1 were significantly upregulated after cold acclimation, indicating an inducible expression in the cold adaptation of Panagrolaimus sp. DAW1. The role of trehalose synthesis genes in Panagrolaimus sp. DAW1 was further investigated by RNAi. Compared to the controls, Pd-tps-2a(RNAi)-treated and cold-acclimated nematodes showed a significant decrease in mRNA, but no change in trehalose content or freezing survival. The involvement of two other trehalose synthesis genes (tps-2b and gob-1) was also investigated. These findings provide the first functional genomic investigation of trehalose synthesis genes in the non-model organism Panagrolaimus sp. DAW1. The presence of several trehalose synthesis genes with different RNAi sensitivities suggests the existence of multiple backup systems in Panagrolaimus sp. DAW1, underlining the importance of this sugar in preparation for freezing. Spreadsheet containing expression dataThis file contains relative expression data for qPCR experiments both before and after RNAi, as well as freezing tolerance and trehalose content.AnnaTrehalose.xlsx |
format |
Dataset |
author |
Seybold, A.C. Wharton, David A. Thorne, Michael A.S. Marshall, Craig J. |
author_facet |
Seybold, A.C. Wharton, David A. Thorne, Michael A.S. Marshall, Craig J. |
author_sort |
Seybold, A.C. |
title |
10.5061/dryad.bc276 |
title_short |
10.5061/dryad.bc276 |
title_full |
10.5061/dryad.bc276 |
title_fullStr |
10.5061/dryad.bc276 |
title_full_unstemmed |
10.5061/dryad.bc276 |
title_sort |
10.5061/dryad.bc276 |
publisher |
Europe PMC |
publishDate |
2019 |
url |
https://doi.org/10.5061/dryad.bc276 |
geographic |
Antarctic McMurdo Sound Ross Island |
geographic_facet |
Antarctic McMurdo Sound Ross Island |
genre |
Antarc* Antarctic Antarctica McMurdo Sound Ross Island |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Sound Ross Island |
op_source |
10.5061/dryad.bc276 oai:easy.dans.knaw.nl:easy-dataset:99865 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:99865 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c re3data_____::r3d100000044 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 |
op_relation |
https://dx.doi.org/10.5061/dryad.bc276 http://dx.doi.org/10.5061/dryad.bc276 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.bc276 |
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1766189958604783616 |