Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research
DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area...
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Online Access: | https://doi.org/10.5061/dryad.sg5s0 |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::b337b475d7a8d7642dad79e15053526f 2023-05-15T14:50:10+02:00 Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. Von Tschirnhaus, M. Roslin, T. 2015-01-01 https://doi.org/10.5061/dryad.sg5s0 undefined unknown http://dx.doi.org/10.5061/dryad.sg5s0 https://dx.doi.org/10.5061/dryad.sg5s0 lic_creative-commons oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:91486 oai:easy.dans.knaw.nl:easy-dataset:91486 10.5061/dryad.sg5s0 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c Life sciences medicine and health care DNA barcode library Greenland species diversity arthropod Zackenberg High Arctic info geo Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2015 fttriple https://doi.org/10.5061/dryad.sg5s0 2023-01-22T16:51:21Z DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community. DS-ZACKANIM intrasp divergence and distance to NN raw dataRaw data on the intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKANIM (dx.doi.org/10.5883/DS-ZACKANIM) and the DNA barcode region CO1DS-ZACKPLAN ITS2 intrasp divergence and distance to NN raw dataRaw data on ... Dataset Arctic Greenland Zackenberg Unknown Arctic Greenland |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
Life sciences medicine and health care DNA barcode library Greenland species diversity arthropod Zackenberg High Arctic info geo |
spellingShingle |
Life sciences medicine and health care DNA barcode library Greenland species diversity arthropod Zackenberg High Arctic info geo Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. Von Tschirnhaus, M. Roslin, T. Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
topic_facet |
Life sciences medicine and health care DNA barcode library Greenland species diversity arthropod Zackenberg High Arctic info geo |
description |
DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community. DS-ZACKANIM intrasp divergence and distance to NN raw dataRaw data on the intraspecific divergence and distance to nearest neighbour for the dataset DS-ZACKANIM (dx.doi.org/10.5883/DS-ZACKANIM) and the DNA barcode region CO1DS-ZACKPLAN ITS2 intrasp divergence and distance to NN raw dataRaw data on ... |
format |
Dataset |
author |
Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. Von Tschirnhaus, M. Roslin, T. |
author_facet |
Wirta, H. Várkonyi, G. Rasmussen, C. Kaartinen, R. Schmidt, N. M. Hebert, P. D. N. Barták, M. Blagoev, G. Disney, H. Ertl, S. Gjelstrup, P. Gwiazdowicz, D. J. Huldén, L. Ilmonen, J. Jakovlev, J. Jaschhof, M. Kahanpää, J. Kankaanpää, T. Krogh, P. H. Labbee, R. Lettner, C. Michelsen, V. Nielsen, S. A. Nielsen, T. R. Paasivirta, L. Pedersen, S. Pohjoismäki, J. Salmela, J. Vilkamaa, P. Väre, H. Von Tschirnhaus, M. Roslin, T. |
author_sort |
Wirta, H. |
title |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_short |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_full |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_fullStr |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_full_unstemmed |
Data from: Establishing a community-wide DNA barcode library as a new tool for arctic research |
title_sort |
data from: establishing a community-wide dna barcode library as a new tool for arctic research |
publishDate |
2015 |
url |
https://doi.org/10.5061/dryad.sg5s0 |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Greenland Zackenberg |
genre_facet |
Arctic Greenland Zackenberg |
op_source |
oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:91486 oai:easy.dans.knaw.nl:easy-dataset:91486 10.5061/dryad.sg5s0 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
http://dx.doi.org/10.5061/dryad.sg5s0 https://dx.doi.org/10.5061/dryad.sg5s0 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.sg5s0 |
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1766321218858778624 |