Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea
Whilst genotype-environment interaction is increasingly receiving attention by ecologists and evolutionary biologists, such studies need genetically homogeneous replicates-a challenging hurdle in outcrossing plants. This could potentially be overcome by using tissue culture techniques. However, plan...
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Online Access: | https://doi.org/10.5061/dryad.9gs8k |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::92d763823963a5c21abf0a2425625f08 2023-05-15T16:52:29+02:00 Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea Kenta, Tanaka Edwards, Jessica E. M. Butlin, Roger K. Burke, Terry Quick, W. Paul Urwin, Peter Davey, Matthew P. 2017-09-19 https://doi.org/10.5061/dryad.9gs8k undefined unknown Dryad https://dx.doi.org/10.5061/dryad.9gs8k http://dx.doi.org/10.5061/dryad.9gs8k lic_creative-commons 10.5061/dryad.9gs8k oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:95458 oai:easy.dans.knaw.nl:easy-dataset:95458 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c genetic architecture Arabidopsis lyrata ssp. petraea stress tolerance Genetic basis adaptive variation reaction norm 2004-2008 Iceland Sweden Norway Life sciences medicine and health care envir info Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2017 fttriple https://doi.org/10.5061/dryad.9gs8k 2023-01-22T17:23:04Z Whilst genotype-environment interaction is increasingly receiving attention by ecologists and evolutionary biologists, such studies need genetically homogeneous replicates-a challenging hurdle in outcrossing plants. This could potentially be overcome by using tissue culture techniques. However, plants regenerated from tissue culture may show aberrant phenotypes and "somaclonal" variation. Here we examined the somaclonal variation due to tissue culturing using the response to cold treatment of the photosynthetic efficiency (chlorophyll fluorescence measurements for Fv/Fm, Fv'/Fm' and ΦPSII, representing maximum efficiency of photosynthesis for dark- and light-adapted leaves, and the actual electron transport operating efficiency, respectively, which are reliable indicators of photoinhibition and damage to the photosynthetic electron transport system). We compared this to variation among half-sibling seedlings from three different families of Arabidopsis lyrata ssp. petraea. Somaclonal variation was limited and we could successfully detect within-family variation in change in chlorophyll fluorescence due to cold shock with the help of tissue-culture derived replicates. Icelandic and Norwegian families exhibited higher chlorophyll fluorescence, suggesting higher performance after cold shock, than a Swedish family. Although the main effect of tissue culture on Fv/Fm, Fv'/Fm' and ΦPSII, was small, there were significant interactions between tissue culture and family, suggesting that the effect of tissue culture is genotype-specific. Tissue-cultured plantlets were less affected by cold treatment than seedlings, but to a different extent in each family. These interactive effects, however, were comparable to, or much smaller than the single effect of family. These results suggest that tissue culture is a useful method for obtaining genetically homogenous replicates for studying genotype-environment interaction related to adaptively-relevant phenotypes, such as cold response, in non-model outcrossing plants. Data for Alp ... Dataset Iceland Unknown Norway |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
genetic architecture Arabidopsis lyrata ssp. petraea stress tolerance Genetic basis adaptive variation reaction norm 2004-2008 Iceland Sweden Norway Life sciences medicine and health care envir info |
spellingShingle |
genetic architecture Arabidopsis lyrata ssp. petraea stress tolerance Genetic basis adaptive variation reaction norm 2004-2008 Iceland Sweden Norway Life sciences medicine and health care envir info Kenta, Tanaka Edwards, Jessica E. M. Butlin, Roger K. Burke, Terry Quick, W. Paul Urwin, Peter Davey, Matthew P. Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea |
topic_facet |
genetic architecture Arabidopsis lyrata ssp. petraea stress tolerance Genetic basis adaptive variation reaction norm 2004-2008 Iceland Sweden Norway Life sciences medicine and health care envir info |
description |
Whilst genotype-environment interaction is increasingly receiving attention by ecologists and evolutionary biologists, such studies need genetically homogeneous replicates-a challenging hurdle in outcrossing plants. This could potentially be overcome by using tissue culture techniques. However, plants regenerated from tissue culture may show aberrant phenotypes and "somaclonal" variation. Here we examined the somaclonal variation due to tissue culturing using the response to cold treatment of the photosynthetic efficiency (chlorophyll fluorescence measurements for Fv/Fm, Fv'/Fm' and ΦPSII, representing maximum efficiency of photosynthesis for dark- and light-adapted leaves, and the actual electron transport operating efficiency, respectively, which are reliable indicators of photoinhibition and damage to the photosynthetic electron transport system). We compared this to variation among half-sibling seedlings from three different families of Arabidopsis lyrata ssp. petraea. Somaclonal variation was limited and we could successfully detect within-family variation in change in chlorophyll fluorescence due to cold shock with the help of tissue-culture derived replicates. Icelandic and Norwegian families exhibited higher chlorophyll fluorescence, suggesting higher performance after cold shock, than a Swedish family. Although the main effect of tissue culture on Fv/Fm, Fv'/Fm' and ΦPSII, was small, there were significant interactions between tissue culture and family, suggesting that the effect of tissue culture is genotype-specific. Tissue-cultured plantlets were less affected by cold treatment than seedlings, but to a different extent in each family. These interactive effects, however, were comparable to, or much smaller than the single effect of family. These results suggest that tissue culture is a useful method for obtaining genetically homogenous replicates for studying genotype-environment interaction related to adaptively-relevant phenotypes, such as cold response, in non-model outcrossing plants. Data for Alp ... |
format |
Dataset |
author |
Kenta, Tanaka Edwards, Jessica E. M. Butlin, Roger K. Burke, Terry Quick, W. Paul Urwin, Peter Davey, Matthew P. |
author_facet |
Kenta, Tanaka Edwards, Jessica E. M. Butlin, Roger K. Burke, Terry Quick, W. Paul Urwin, Peter Davey, Matthew P. |
author_sort |
Kenta, Tanaka |
title |
Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea |
title_short |
Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea |
title_full |
Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea |
title_fullStr |
Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea |
title_full_unstemmed |
Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea |
title_sort |
data from: tissue culture as a source of replicates in non-model plants: variation in cold response in arabidopsis lyrata ssp. petraea |
publisher |
Dryad |
publishDate |
2017 |
url |
https://doi.org/10.5061/dryad.9gs8k |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
10.5061/dryad.9gs8k oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:95458 oai:easy.dans.knaw.nl:easy-dataset:95458 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
https://dx.doi.org/10.5061/dryad.9gs8k http://dx.doi.org/10.5061/dryad.9gs8k |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.9gs8k |
_version_ |
1766042777228935168 |