Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease
Canine myxomatous mitral valve disease (MMVD) resembles the early stages of myxomatous pathology seen in human non-syndromic mitral valve prolapse, a common valvular heart disease in the adult human population. Canine MMVD is seen in older subjects, suggesting age-related epigenetic dysregulation le...
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Online Access: | https://doi.org/10.5061/dryad.3274k |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::8e4e0e1ccde23d1d1fe496f65a3b6c58 2023-05-15T15:50:40+02:00 Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease Yang, Vicky K. Tai, Albert K. Huh, Terry P. Meola, Dawn M. Juhr, Christine M. Robinson, Nicholas A. Hoffman, Andrew M. 2017-01-01 https://doi.org/10.5061/dryad.3274k undefined unknown Data Archiving and Networked Services (DANS) http://dx.doi.org/10.5061/dryad.3274k https://dx.doi.org/10.5061/dryad.3274k lic_creative-commons oai:easy.dans.knaw.nl:easy-dataset:98300 10.5061/dryad.3274k oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:98300 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 Life sciences medicine and health care Canis lupus familiaris socio envir Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2017 fttriple https://doi.org/10.5061/dryad.3274k 2023-01-22T16:53:26Z Canine myxomatous mitral valve disease (MMVD) resembles the early stages of myxomatous pathology seen in human non-syndromic mitral valve prolapse, a common valvular heart disease in the adult human population. Canine MMVD is seen in older subjects, suggesting age-related epigenetic dysregulation leading to derangements in valvular cell populations and matrix synthesis or degradation. We hypothesized that valvular interstitial cells (VICs) undergo disease-relevant changes in miRNA expression. In primary VIC lines from diseased and control valves, miRNA expression was profiled using RT-qPCR and next generation sequencing. VICs from diseased valves showed phenotypic changes consistent with myofibroblastic differentiation (vimentinlow+, a-SMAhigh+), increases in senescence markers (p21, SA-b-gal), and decreased cell viability and proliferation potential. RT-qPCR and miRNA sequencing analyses both showed significant (p<0.05) downregulation of let-7c, miR-17, miR-20a, and miR-30d in VICs from diseased valves compared to controls. Decreased let-7c, miR-17, and miR-20a may contribute to myofibroblastic differentiation in addition to cell senescence, and decreased miR-30d may disinhibit cell apoptosis. These data support the hypothesis that epigenetic dysregulation plays an important role in age-related canine MMVD. RNAseq dataExcel data file with RNAseq count dataDESeq2_normalized_data.xlsxRT-qPCR DataPCR data with Ct numbersVICs PCR Data.xlsx Dataset Canis lupus Unknown |
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Open Polar |
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Unknown |
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Life sciences medicine and health care Canis lupus familiaris socio envir |
spellingShingle |
Life sciences medicine and health care Canis lupus familiaris socio envir Yang, Vicky K. Tai, Albert K. Huh, Terry P. Meola, Dawn M. Juhr, Christine M. Robinson, Nicholas A. Hoffman, Andrew M. Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease |
topic_facet |
Life sciences medicine and health care Canis lupus familiaris socio envir |
description |
Canine myxomatous mitral valve disease (MMVD) resembles the early stages of myxomatous pathology seen in human non-syndromic mitral valve prolapse, a common valvular heart disease in the adult human population. Canine MMVD is seen in older subjects, suggesting age-related epigenetic dysregulation leading to derangements in valvular cell populations and matrix synthesis or degradation. We hypothesized that valvular interstitial cells (VICs) undergo disease-relevant changes in miRNA expression. In primary VIC lines from diseased and control valves, miRNA expression was profiled using RT-qPCR and next generation sequencing. VICs from diseased valves showed phenotypic changes consistent with myofibroblastic differentiation (vimentinlow+, a-SMAhigh+), increases in senescence markers (p21, SA-b-gal), and decreased cell viability and proliferation potential. RT-qPCR and miRNA sequencing analyses both showed significant (p<0.05) downregulation of let-7c, miR-17, miR-20a, and miR-30d in VICs from diseased valves compared to controls. Decreased let-7c, miR-17, and miR-20a may contribute to myofibroblastic differentiation in addition to cell senescence, and decreased miR-30d may disinhibit cell apoptosis. These data support the hypothesis that epigenetic dysregulation plays an important role in age-related canine MMVD. RNAseq dataExcel data file with RNAseq count dataDESeq2_normalized_data.xlsxRT-qPCR DataPCR data with Ct numbersVICs PCR Data.xlsx |
format |
Dataset |
author |
Yang, Vicky K. Tai, Albert K. Huh, Terry P. Meola, Dawn M. Juhr, Christine M. Robinson, Nicholas A. Hoffman, Andrew M. |
author_facet |
Yang, Vicky K. Tai, Albert K. Huh, Terry P. Meola, Dawn M. Juhr, Christine M. Robinson, Nicholas A. Hoffman, Andrew M. |
author_sort |
Yang, Vicky K. |
title |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease |
title_short |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease |
title_full |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease |
title_fullStr |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease |
title_full_unstemmed |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease |
title_sort |
data from: dysregulation of valvular interstitial cell let-7c, mir-17, mir-20a, and mir-30d in naturally occurring canine myxomatous mitral valve disease |
publisher |
Data Archiving and Networked Services (DANS) |
publishDate |
2017 |
url |
https://doi.org/10.5061/dryad.3274k |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
oai:easy.dans.knaw.nl:easy-dataset:98300 10.5061/dryad.3274k oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:98300 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 |
op_relation |
http://dx.doi.org/10.5061/dryad.3274k https://dx.doi.org/10.5061/dryad.3274k |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.3274k |
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1766385666362441728 |