Data from: Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA

• Main Authors: Spangler, Mark A., Huettmann, Falk, Herriott, Ian C., López, J. Andrés
• Format: Dataset
• Language: unknown
• Published: 2017
• Subjects: Life sciences; medicine and health care; Rana sylvatica; wood frog; eDNA; cytochrome b; Alaska; Fairbanks North Star Borough; envir; geo; Fairbanks; North Pole; North Star
• Online Access: https://doi.org/10.5061/dryad.b7g24

We developed and describe a qPCR assay for the detection of wood frogs (Rana sylvatica) using environmental DNA (eDNA) sampling. A single primer set was designed to amplify a 115-bp region of the wood frog cytochrome B gene and assessed for target specificity. There was no evidence of amplification...