Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise ( Phocoena phocoena ),...
Published in: | Royal Society Open Science |
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Format: | Article in Journal/Newspaper |
Language: | English |
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The Royal Society
2018
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Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180537 https://doi.org/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/10.1098/rsos.180537 https://www.ncbi.nlm.nih.gov/pubmed/30225045 https://repository.library.noaa.gov/view/noaa/24657 https://rsos.royalsocietypublishing.org/content/5/8/180537 https://academic.microsoft.com/#/detail/2876551264 |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::8b1f18cf1b03e1f1632861b77fc49eba 2023-05-15T16:33:25+02:00 Water, water everywhere: environmental DNA can unlock population structure in elusive marine species Kim M. Parsons Linda Park Meredith Everett Marilyn Dahlheim 2018-08-08 https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180537 https://doi.org/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/10.1098/rsos.180537 https://www.ncbi.nlm.nih.gov/pubmed/30225045 https://repository.library.noaa.gov/view/noaa/24657 https://rsos.royalsocietypublishing.org/content/5/8/180537 https://academic.microsoft.com/#/detail/2876551264 en eng The Royal Society https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180537 http://dx.doi.org/10.1098/rsos.180537 https://dx.doi.org/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/10.1098/rsos.180537 https://www.ncbi.nlm.nih.gov/pubmed/30225045 https://repository.library.noaa.gov/view/noaa/24657 https://rsos.royalsocietypublishing.org/content/5/8/180537 https://academic.microsoft.com/#/detail/2876551264 undefined oai:doaj.org/article:d5d22749223946c59068866fdbb9fd2d 10.1098/rsos.180537 2876551264 30225045 10|driver______::bee53aa31dc2cbb538c10c2b65fa5824 10|doajarticles::c215d7df6759ca83f13aab2c3ea6da81 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|openaire____::55045bd2a65019fd8e6741a755395c8c 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|openaire____::5f532a3fc4f1ea403f37070f59a7a53a 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c 10|openaire____::8ac8380272269217cb09a928c8caa993 porpoise environmental dna population genetics cetacean stock next-generation sequencing envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2018 fttriple https://doi.org/10.1098/rsos.180537 2023-01-22T17:15:13Z Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise ( Phocoena phocoena ), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries by-catch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next-generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. Article in Journal/Newspaper Harbour porpoise Phocoena phocoena Alaska Unknown Royal Society Open Science 5 8 180537 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
porpoise environmental dna population genetics cetacean stock next-generation sequencing envir geo |
spellingShingle |
porpoise environmental dna population genetics cetacean stock next-generation sequencing envir geo Kim M. Parsons Linda Park Meredith Everett Marilyn Dahlheim Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
topic_facet |
porpoise environmental dna population genetics cetacean stock next-generation sequencing envir geo |
description |
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise ( Phocoena phocoena ), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries by-catch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next-generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. |
format |
Article in Journal/Newspaper |
author |
Kim M. Parsons Linda Park Meredith Everett Marilyn Dahlheim |
author_facet |
Kim M. Parsons Linda Park Meredith Everett Marilyn Dahlheim |
author_sort |
Kim M. Parsons |
title |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_short |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_fullStr |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full_unstemmed |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_sort |
water, water everywhere: environmental dna can unlock population structure in elusive marine species |
publisher |
The Royal Society |
publishDate |
2018 |
url |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180537 https://doi.org/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/10.1098/rsos.180537 https://www.ncbi.nlm.nih.gov/pubmed/30225045 https://repository.library.noaa.gov/view/noaa/24657 https://rsos.royalsocietypublishing.org/content/5/8/180537 https://academic.microsoft.com/#/detail/2876551264 |
genre |
Harbour porpoise Phocoena phocoena Alaska |
genre_facet |
Harbour porpoise Phocoena phocoena Alaska |
op_source |
oai:doaj.org/article:d5d22749223946c59068866fdbb9fd2d 10.1098/rsos.180537 2876551264 30225045 10|driver______::bee53aa31dc2cbb538c10c2b65fa5824 10|doajarticles::c215d7df6759ca83f13aab2c3ea6da81 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|openaire____::55045bd2a65019fd8e6741a755395c8c 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|openaire____::5f532a3fc4f1ea403f37070f59a7a53a 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c 10|openaire____::8ac8380272269217cb09a928c8caa993 |
op_relation |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180537 http://dx.doi.org/10.1098/rsos.180537 https://dx.doi.org/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/10.1098/rsos.180537 https://www.ncbi.nlm.nih.gov/pubmed/30225045 https://repository.library.noaa.gov/view/noaa/24657 https://rsos.royalsocietypublishing.org/content/5/8/180537 https://academic.microsoft.com/#/detail/2876551264 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1098/rsos.180537 |
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Royal Society Open Science |
container_volume |
5 |
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container_start_page |
180537 |
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