Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly und...
Main Authors: | , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
2018
|
Subjects: | |
Online Access: | https://doi.org/10.5061/dryad.kn45n49 |
id |
fttriple:oai:gotriple.eu:50|dedup_wf_001::8704896713467200caa4a275a3168393 |
---|---|
record_format |
openpolar |
spelling |
fttriple:oai:gotriple.eu:50|dedup_wf_001::8704896713467200caa4a275a3168393 2023-05-15T15:57:47+02:00 Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. 2018-01-01 https://doi.org/10.5061/dryad.kn45n49 undefined unknown http://dx.doi.org/10.5061/dryad.kn45n49 https://dx.doi.org/10.5061/dryad.kn45n49 lic_creative-commons oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:113704 10.5061/dryad.kn45n49 oai:easy.dans.knaw.nl:easy-dataset:113704 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f Life sciences medicine and health care Ussing chamber technique calcification Ca2+ kinetics biomineralisation Ca2+ transport Crassostrea gigas geo droit Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2018 fttriple https://doi.org/10.5061/dryad.kn45n49 2023-01-22T16:53:37Z Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a Km of 6.2 mM and a Vmax of 3.3 nM min−1. The transcellular transfer of Ca2+ accounts for approximately 60% of the total Ca2+ transfer across the OME of C. gigas at environmental Ca2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca2+-channels, plasma-membrane Ca2+-ATPase and Na+/Ca2+-exchanger all participate in the transcellular Ca2+ transfer across the OME and a model for this Ca2+ transfer is presented and discussed. Electronic data for kinetics studyThe electronic raw data for kinetics experimentElectronic data caloxinElectronic data ORMElectronic data orm.xlsxElectronic data ouabainElectronic data vanadateElectronic data verapamilDPM kinetics studyDPM kinetics.xlsxDPM caloxinDPM ORMDPM ouabainDPM vanadateDPM verapamil Dataset Crassostrea gigas Pacific oyster Unknown Pacific |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
Life sciences medicine and health care Ussing chamber technique calcification Ca2+ kinetics biomineralisation Ca2+ transport Crassostrea gigas geo droit |
spellingShingle |
Life sciences medicine and health care Ussing chamber technique calcification Ca2+ kinetics biomineralisation Ca2+ transport Crassostrea gigas geo droit Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
topic_facet |
Life sciences medicine and health care Ussing chamber technique calcification Ca2+ kinetics biomineralisation Ca2+ transport Crassostrea gigas geo droit |
description |
Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca2+) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies revealed that the Ca2+ transfer across the OME consists of one saturable and one linear component, of which the saturable component fits best to Michaelis–Menten kinetics and is characterized by a Km of 6.2 mM and a Vmax of 3.3 nM min−1. The transcellular transfer of Ca2+ accounts for approximately 60% of the total Ca2+ transfer across the OME of C. gigas at environmental Ca2+ concentrations. The use of the pharmacological inhibitors: verapamil, ouabain and caloxin 1a1 revealed that voltage-gated Ca2+-channels, plasma-membrane Ca2+-ATPase and Na+/Ca2+-exchanger all participate in the transcellular Ca2+ transfer across the OME and a model for this Ca2+ transfer is presented and discussed. Electronic data for kinetics studyThe electronic raw data for kinetics experimentElectronic data caloxinElectronic data ORMElectronic data orm.xlsxElectronic data ouabainElectronic data vanadateElectronic data verapamilDPM kinetics studyDPM kinetics.xlsxDPM caloxinDPM ORMDPM ouabainDPM vanadateDPM verapamil |
format |
Dataset |
author |
Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. |
author_facet |
Sillanpää, J. Kirsikka Sundh, Henrik Sundell, Kristina S. |
author_sort |
Sillanpää, J. Kirsikka |
title |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_short |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_full |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Data from: Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas |
title_sort |
data from: calcium transfer across the outer mantle epithelium in the pacific oyster, crassostrea gigas |
publishDate |
2018 |
url |
https://doi.org/10.5061/dryad.kn45n49 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:113704 10.5061/dryad.kn45n49 oai:easy.dans.knaw.nl:easy-dataset:113704 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f |
op_relation |
http://dx.doi.org/10.5061/dryad.kn45n49 https://dx.doi.org/10.5061/dryad.kn45n49 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.kn45n49 |
_version_ |
1766393473718550528 |