Data from: A test of somatic mosaicism in the androgen receptor gene of Canada lynx (Lynx canadensis)

• Main Authors: Prentice, Melanie B., Bowman, Jeff, Wilson, Paul J.
• Format: Dataset
• Language: unknown
• Published: Data Archiving and Networked Services (DANS) 2016
• Subjects: Life sciences; medicine and health care; Lynx canadensis; androgen receptor; somatic mosaicism; Canada lynx; trinucleotide repeats; Holocene; Canada; envir; archeo; Lynx; Lynx lynx lynx
• Online Access: https://doi.org/10.5061/dryad.h43c1

Background: The androgen receptor, an X-linked gene, has been widely studied in human populations because it contains highly polymorphic trinucleotide repeat motifs that have been associated with a number of adverse human health and behavioral effects. A previous study on the androgen receptor gene in carnivores reported somatic mosaicism in the tissues of a number of species including Eurasian lynx (Lynx lynx). We investigated this claim in a closely related species, Canada lynx (Lynx canadensis). The presence of somatic mosaicism in lynx tissues could have implications for the future study of exonic trinucleotide repeats in landscape genomic studies, in which the accurate reporting of genotypes would be highly problematic. Methods: To determine whether mosaicism occurs in Canada lynx, two lynx individuals were sampled for a variety of tissue types (lynx 1) and tissue locations (lynx 1 and 2), and 1,672 individuals of known sex were genotyped to further rule out mosaicism. Results: We found no evidence of mosaicism in tissues from the two necropsied individuals, or any of our genotyped samples. Conclusions: Our results indicate that mosaicism does not manifest in Canada lynx. Therefore, the use of hide samples for further work involving trinucleotide repeat polymorphisms in Canada lynx is warranted. Androgen Receptor Genotype Data for Canada lynx Somatic Mosaicism Manuscript_2015This data file contains Canada lynx genotype data that was generated for the associated manuscript, which tests for the presence of somatic mosaicism of trinucleotide repeats within the Androgen Receptor (AR) gene. The data file contains all samples analyzed for this manuscript with the exception of one sample which was determined to be contaminated and removed from the data set (see Supplementary Information document associated with the manuscript for further details). As the AR gene is X-linked, gender data is also provided for each sample.