Validating the Incorporation of 13C and 15N in a Shorebird That Consumes an Isotopically Distinct Chemosymbiotic Bivalve
The wealth of field studies using stable isotopes to make inferences about animal diets require controlled validation experiments to make proper interpretations. Despite several pleas in the literature for such experiments, validation studies are still lagging behind, notably in consumers dwelling i...
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Open Polar |
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English |
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Research Article General Biochemistry Genetics and Molecular Biology General Agricultural and Biological Sciences General Medicine envir psy |
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Research Article General Biochemistry Genetics and Molecular Biology General Agricultural and Biological Sciences General Medicine envir psy Jan A. van Gils Mohamed Vall Ahmedou Salem Validating the Incorporation of 13C and 15N in a Shorebird That Consumes an Isotopically Distinct Chemosymbiotic Bivalve |
topic_facet |
Research Article General Biochemistry Genetics and Molecular Biology General Agricultural and Biological Sciences General Medicine envir psy |
description |
The wealth of field studies using stable isotopes to make inferences about animal diets require controlled validation experiments to make proper interpretations. Despite several pleas in the literature for such experiments, validation studies are still lagging behind, notably in consumers dwelling in chemosynthesis-based ecosystems. In this paper we present such a validation experiment for the incorporation of 13C and 15N in the blood plasma of a medium-sized shorebird, the red knot (Calidris canutus canutus), consuming a chemosymbiotic lucinid bivalve (Loripes lucinalis). Because this bivalve forms a symbiosis with chemoautotrophic sulphide-oxidizing bacteria living inside its gill, the bivalve is isotopically distinct from 'normal' bivalves whose food has a photosynthetic basis. Here we experimentally tested the hypothesis that isotope discrimination and incorporation dynamics are different when consuming such chemosynthesis-based prey. The experiment showed that neither the isotopic discrimination factor, nor isotopic turnover time, differed between birds consuming the chemosymbiotic lucinid and a control group consuming a photosynthesis-based bivalve. This was true for 13C as well as for 15N. However, in both groups the 15N discrimination factor was much higher than expected, which probably had to do with the birds losing body mass over the course of the experiment. |
format |
Article in Journal/Newspaper |
author |
Jan A. van Gils Mohamed Vall Ahmedou Salem |
author_facet |
Jan A. van Gils Mohamed Vall Ahmedou Salem |
author_sort |
Jan A. van Gils |
title |
Validating the Incorporation of 13C and 15N in a Shorebird That Consumes an Isotopically Distinct Chemosymbiotic Bivalve |
title_short |
Validating the Incorporation of 13C and 15N in a Shorebird That Consumes an Isotopically Distinct Chemosymbiotic Bivalve |
title_full |
Validating the Incorporation of 13C and 15N in a Shorebird That Consumes an Isotopically Distinct Chemosymbiotic Bivalve |
title_fullStr |
Validating the Incorporation of 13C and 15N in a Shorebird That Consumes an Isotopically Distinct Chemosymbiotic Bivalve |
title_full_unstemmed |
Validating the Incorporation of 13C and 15N in a Shorebird That Consumes an Isotopically Distinct Chemosymbiotic Bivalve |
title_sort |
validating the incorporation of 13c and 15n in a shorebird that consumes an isotopically distinct chemosymbiotic bivalve |
publisher |
Public Library of Science |
publishDate |
2015 |
url |
http://www.vliz.be/imisdocs/publications/29/278429.pdf http://europepmc.org/articles/PMC4601768 https://doi.org/10.1371/journal.pone.0140221 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0140221&type=printable https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601768/ https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0140221 https://pubmed.ncbi.nlm.nih.gov/26458005/ https://www.narcis.nl/publication/RecordID/oai%3Aimis.nioz.nl%3A250508 https://ui.adsabs.harvard.edu/abs/2015PLoSO.1040221V/abstract https://paperity.org/p/74267331/validating-the-incorporation-of-13c-and-15n-in-a-shorebird-that-consumes-an-isotopically http://imis.nioz.nl/imis.php?module=ref&refid=250508 https://academic.microsoft.com/#/detail/2209100139 http://europepmc.org/articles/PMC4601768?pdf=render |
genre |
Calidris canutus Red Knot |
genre_facet |
Calidris canutus Red Knot |
op_source |
oai:pubmedcentral.nih.gov:4601768 10.1371/journal.pone.0140221 26458005 oai:imis.nioz.nl:250508 oai:services.nod.dans.knaw.nl:Publications/nioz:oai:imis.nioz.nl:250508 2209100139 oai:doaj.org/article:b1a21c402fd0440d820c8f7b912b7d07 nioz:oai:imis.nioz.nl:250508 10|opendoar____::eda80a3d5b344bc40f3bc04f65b7a357 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 openaire____::1256f046-bf1f-4afc-8b47-d0b147148b18 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|openaire____::55045bd2a65019fd8e6741a755395c8c 10|opendoar____::f4aa0dd960521e045ae2f20621fb4ee9 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|doajarticles::830e55b42c4aaa815c19cfa4f2e5855e 10|openaire____::8ac8380272269217cb09a928c8caa993 10|openaire____::5f532a3fc4f1ea403f37070f59a7a53a 10|driver______::bee53aa31dc2cbb538c10c2b65fa5824 10|openaire____::fdb035c8b3e0540a8d9a561a6c44f4de 10|openaire____::806360c771262b4d6770e7cdf04b5c5a |
op_relation |
http://www.vliz.be/imisdocs/publications/29/278429.pdf http://europepmc.org/articles/PMC4601768 https://dx.doi.org/10.1371/journal.pone.0140221 http://dx.doi.org/10.1371/journal.pone.0140221 http://dx.plos.org/10.1371/journal.pone.0140221 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0140221&type=printable https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601768/ https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0140221 https://pubmed.ncbi.nlm.nih.gov/26458005/ https://www.narcis.nl/publication/RecordID/oai%3Aimis.nioz.nl%3A250508 https://ui.adsabs.harvard.edu/abs/2015PLoSO.1040221V/abstract https://dx.plos.org/10.1371/journal.pone.0140221 https://paperity.org/p/74267331/validating-the-incorporation-of-13c-and-15n-in-a-shorebird-that-consumes-an-isotopically http://imis.nioz.nl/imis.php?module=ref&refid=250508 https://academic.microsoft.com/#/detail/2209100139 http://europepmc.org/articles/PMC4601768?pdf=render |
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https://doi.org/10.1371/journal.pone.0140221 |
container_title |
PLOS ONE |
container_volume |
10 |
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10 |
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e0140221 |
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1766383417576914944 |
spelling |
fttriple:oai:gotriple.eu:50|dedup_wf_001::85acac0ba18ad9fd9ab4f641d4f7c246 2023-05-15T15:48:27+02:00 Validating the Incorporation of 13C and 15N in a Shorebird That Consumes an Isotopically Distinct Chemosymbiotic Bivalve Jan A. van Gils Mohamed Vall Ahmedou Salem 2015-10-12 http://www.vliz.be/imisdocs/publications/29/278429.pdf http://europepmc.org/articles/PMC4601768 https://doi.org/10.1371/journal.pone.0140221 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0140221&type=printable https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601768/ https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0140221 https://pubmed.ncbi.nlm.nih.gov/26458005/ https://www.narcis.nl/publication/RecordID/oai%3Aimis.nioz.nl%3A250508 https://ui.adsabs.harvard.edu/abs/2015PLoSO.1040221V/abstract https://paperity.org/p/74267331/validating-the-incorporation-of-13c-and-15n-in-a-shorebird-that-consumes-an-isotopically http://imis.nioz.nl/imis.php?module=ref&refid=250508 https://academic.microsoft.com/#/detail/2209100139 http://europepmc.org/articles/PMC4601768?pdf=render en eng Public Library of Science http://www.vliz.be/imisdocs/publications/29/278429.pdf http://europepmc.org/articles/PMC4601768 https://dx.doi.org/10.1371/journal.pone.0140221 http://dx.doi.org/10.1371/journal.pone.0140221 http://dx.plos.org/10.1371/journal.pone.0140221 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0140221&type=printable https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4601768/ https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0140221 https://pubmed.ncbi.nlm.nih.gov/26458005/ https://www.narcis.nl/publication/RecordID/oai%3Aimis.nioz.nl%3A250508 https://ui.adsabs.harvard.edu/abs/2015PLoSO.1040221V/abstract https://dx.plos.org/10.1371/journal.pone.0140221 https://paperity.org/p/74267331/validating-the-incorporation-of-13c-and-15n-in-a-shorebird-that-consumes-an-isotopically http://imis.nioz.nl/imis.php?module=ref&refid=250508 https://academic.microsoft.com/#/detail/2209100139 http://europepmc.org/articles/PMC4601768?pdf=render lic_creative-commons oai:pubmedcentral.nih.gov:4601768 10.1371/journal.pone.0140221 26458005 oai:imis.nioz.nl:250508 oai:services.nod.dans.knaw.nl:Publications/nioz:oai:imis.nioz.nl:250508 2209100139 oai:doaj.org/article:b1a21c402fd0440d820c8f7b912b7d07 nioz:oai:imis.nioz.nl:250508 10|opendoar____::eda80a3d5b344bc40f3bc04f65b7a357 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 openaire____::1256f046-bf1f-4afc-8b47-d0b147148b18 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|openaire____::55045bd2a65019fd8e6741a755395c8c 10|opendoar____::f4aa0dd960521e045ae2f20621fb4ee9 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|doajarticles::830e55b42c4aaa815c19cfa4f2e5855e 10|openaire____::8ac8380272269217cb09a928c8caa993 10|openaire____::5f532a3fc4f1ea403f37070f59a7a53a 10|driver______::bee53aa31dc2cbb538c10c2b65fa5824 10|openaire____::fdb035c8b3e0540a8d9a561a6c44f4de 10|openaire____::806360c771262b4d6770e7cdf04b5c5a Research Article General Biochemistry Genetics and Molecular Biology General Agricultural and Biological Sciences General Medicine envir psy Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2015 fttriple https://doi.org/10.1371/journal.pone.0140221 2023-01-22T17:22:02Z The wealth of field studies using stable isotopes to make inferences about animal diets require controlled validation experiments to make proper interpretations. Despite several pleas in the literature for such experiments, validation studies are still lagging behind, notably in consumers dwelling in chemosynthesis-based ecosystems. In this paper we present such a validation experiment for the incorporation of 13C and 15N in the blood plasma of a medium-sized shorebird, the red knot (Calidris canutus canutus), consuming a chemosymbiotic lucinid bivalve (Loripes lucinalis). Because this bivalve forms a symbiosis with chemoautotrophic sulphide-oxidizing bacteria living inside its gill, the bivalve is isotopically distinct from 'normal' bivalves whose food has a photosynthetic basis. Here we experimentally tested the hypothesis that isotope discrimination and incorporation dynamics are different when consuming such chemosynthesis-based prey. The experiment showed that neither the isotopic discrimination factor, nor isotopic turnover time, differed between birds consuming the chemosymbiotic lucinid and a control group consuming a photosynthesis-based bivalve. This was true for 13C as well as for 15N. However, in both groups the 15N discrimination factor was much higher than expected, which probably had to do with the birds losing body mass over the course of the experiment. Article in Journal/Newspaper Calidris canutus Red Knot Unknown PLOS ONE 10 10 e0140221 |