Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis
None: Mosquito-borne infectious diseases are emerging in many regions of the world. Consequently, surveillance of mosquitoes and concomitant infectious agents is of great importance for prediction and prevention of mosquito-borne infectious diseases. Currently, morphological identification of mosqui...
Published in: | Molecular Ecology Resources |
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Online Access: | https://doi.org/10.1111/1755-0998.12202 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12202 https://www.ncbi.nlm.nih.gov/pubmed/24215491 https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.12202 http://umu.diva-portal.org/smash/record.jsf?pid=diva2:718954 http://www.diva-portal.org/smash/record.jsf?pid=diva2:719234 http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12202/abstract https://academic.microsoft.com/#/detail/2091777117 |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::822f4d6a294905aaa0a99af91cc04aef 2023-05-15T17:44:51+02:00 Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis Mayra Bravo Jonas Näslund Pär Larsson Clas Ahlm Jan O. Lundström Cecilia Engdahl Göran Bucht Magnus Evander 2013-11-12 https://doi.org/10.1111/1755-0998.12202 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12202 https://www.ncbi.nlm.nih.gov/pubmed/24215491 https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.12202 http://umu.diva-portal.org/smash/record.jsf?pid=diva2:718954 http://www.diva-portal.org/smash/record.jsf?pid=diva2:719234 http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12202/abstract https://academic.microsoft.com/#/detail/2091777117 undefined unknown Wiley http://dx.doi.org/10.1111/1755-0998.12202 https://dx.doi.org/10.1111/1755-0998.12202 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12202 https://www.ncbi.nlm.nih.gov/pubmed/24215491 https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.12202 http://umu.diva-portal.org/smash/record.jsf?pid=diva2:718954 http://www.diva-portal.org/smash/record.jsf?pid=diva2:719234 http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12202/abstract https://academic.microsoft.com/#/detail/2091777117 undefined 10.1111/1755-0998.12202 2091777117 24215491 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|openaire____::55045bd2a65019fd8e6741a755395c8c 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|issn___print::b218938d02cd90e10a9c0a89780b4300 10|openaire____::5f532a3fc4f1ea403f37070f59a7a53a openaire____::1256f046-bf1f-4afc-8b47-d0b147148b18 10|openaire____::806360c771262b4d6770e7cdf04b5c5a 10|opendoar____::eda80a3d5b344bc40f3bc04f65b7a357 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c Biotechnology Genetics Ecology Evolution Behavior and Systematics stat envir Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2013 fttriple https://doi.org/10.1111/1755-0998.12202 2023-01-22T17:15:59Z None: Mosquito-borne infectious diseases are emerging in many regions of the world. Consequently, surveillance of mosquitoes and concomitant infectious agents is of great importance for prediction and prevention of mosquito-borne infectious diseases. Currently, morphological identification of mosquitoes is the traditional procedure. However, sequencing of specified genes or standard genomic regions, DNA barcoding, has recently been suggested as a global standard for identification and classification of many different species. Our aim was to develop a genetic method to identify mosquitoes and to study their relationship. Mosquitoes were captured at collection sites in northern Sweden and identified morphologically before the cytochrome c oxidase subunit I (COI) gene sequences of 14 of the most common mosquito species were determined. The sequences obtained were then used for phylogenetic placement, for validation and benchmarking of phenetic classifications and finally to develop a hierarchical PCR-based typing scheme based on single nucleotide polymorphism sites (SNPs) to enable rapid genetic identification, circumventing the need for morphological characterization. The results showed that exact phylogenetic relationships between mosquito taxa were preserved at shorter evolutionary distances, but at deeper levels, they could not be inferred with confidence using COI gene sequence data alone. Fourteen of the most common mosquito species in Sweden were identified by the SNP/PCR-based typing scheme, demonstrating that genetic typing using SNPs of the COI gene is a useful method for identification of mosquitoes with potential for worldwide application. Article in Journal/Newspaper Northern Sweden Unknown Molecular Ecology Resources 14 3 478 488 |
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Biotechnology Genetics Ecology Evolution Behavior and Systematics stat envir |
spellingShingle |
Biotechnology Genetics Ecology Evolution Behavior and Systematics stat envir Mayra Bravo Jonas Näslund Pär Larsson Clas Ahlm Jan O. Lundström Cecilia Engdahl Göran Bucht Magnus Evander Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
topic_facet |
Biotechnology Genetics Ecology Evolution Behavior and Systematics stat envir |
description |
None: Mosquito-borne infectious diseases are emerging in many regions of the world. Consequently, surveillance of mosquitoes and concomitant infectious agents is of great importance for prediction and prevention of mosquito-borne infectious diseases. Currently, morphological identification of mosquitoes is the traditional procedure. However, sequencing of specified genes or standard genomic regions, DNA barcoding, has recently been suggested as a global standard for identification and classification of many different species. Our aim was to develop a genetic method to identify mosquitoes and to study their relationship. Mosquitoes were captured at collection sites in northern Sweden and identified morphologically before the cytochrome c oxidase subunit I (COI) gene sequences of 14 of the most common mosquito species were determined. The sequences obtained were then used for phylogenetic placement, for validation and benchmarking of phenetic classifications and finally to develop a hierarchical PCR-based typing scheme based on single nucleotide polymorphism sites (SNPs) to enable rapid genetic identification, circumventing the need for morphological characterization. The results showed that exact phylogenetic relationships between mosquito taxa were preserved at shorter evolutionary distances, but at deeper levels, they could not be inferred with confidence using COI gene sequence data alone. Fourteen of the most common mosquito species in Sweden were identified by the SNP/PCR-based typing scheme, demonstrating that genetic typing using SNPs of the COI gene is a useful method for identification of mosquitoes with potential for worldwide application. |
format |
Article in Journal/Newspaper |
author |
Mayra Bravo Jonas Näslund Pär Larsson Clas Ahlm Jan O. Lundström Cecilia Engdahl Göran Bucht Magnus Evander |
author_facet |
Mayra Bravo Jonas Näslund Pär Larsson Clas Ahlm Jan O. Lundström Cecilia Engdahl Göran Bucht Magnus Evander |
author_sort |
Mayra Bravo |
title |
Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_short |
Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_full |
Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_fullStr |
Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_full_unstemmed |
Identification of Swedish mosquitoes based on molecular barcoding of the COI gene and SNP analysis |
title_sort |
identification of swedish mosquitoes based on molecular barcoding of the coi gene and snp analysis |
publisher |
Wiley |
publishDate |
2013 |
url |
https://doi.org/10.1111/1755-0998.12202 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12202 https://www.ncbi.nlm.nih.gov/pubmed/24215491 https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.12202 http://umu.diva-portal.org/smash/record.jsf?pid=diva2:718954 http://www.diva-portal.org/smash/record.jsf?pid=diva2:719234 http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12202/abstract https://academic.microsoft.com/#/detail/2091777117 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
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op_relation |
http://dx.doi.org/10.1111/1755-0998.12202 https://dx.doi.org/10.1111/1755-0998.12202 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12202 https://www.ncbi.nlm.nih.gov/pubmed/24215491 https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.12202 http://umu.diva-portal.org/smash/record.jsf?pid=diva2:718954 http://www.diva-portal.org/smash/record.jsf?pid=diva2:719234 http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12202/abstract https://academic.microsoft.com/#/detail/2091777117 |
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Molecular Ecology Resources |
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