Data from: Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera)
DNA preserved in degraded beetle (Coleoptera) specimens, including those derived from dry-stored museum and ancient permafrost-preserved environments, could provide a valuable resource for researchers interested in species and population histories over timescales from decades to millenia. However, t...
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fttriple:oai:gotriple.eu:50|dedup_wf_001::80c3962edc3abaadab4d3a0453b7d91b 2023-05-15T17:58:16+02:00 Data from: Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera) Heintzman, Peter D. Elias, Scott A. Moore, Karen Paszkiewicz, Konrad Barnes, Ian 2013-01-01 https://doi.org/10.5061/dryad.0179t undefined unknown http://dx.doi.org/10.5061/dryad.0179t https://dx.doi.org/10.5061/dryad.0179t lic_creative-commons oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:84283 10.5061/dryad.0179t oai:easy.dans.knaw.nl:easy-dataset:84283 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c Life sciences medicine and health care metagenomics Late Pleistocene Quaternary Coleoptera Insecta shotgun sequencing Carabidae ancient DNA Recent DNA preservation Wolbachia museum DNA Spiroplasma Holocene Amara alpina Scandinavia North America Russia Canada Sweden archeo museo Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2013 fttriple https://doi.org/10.5061/dryad.0179t 2023-01-22T16:53:24Z DNA preserved in degraded beetle (Coleoptera) specimens, including those derived from dry-stored museum and ancient permafrost-preserved environments, could provide a valuable resource for researchers interested in species and population histories over timescales from decades to millenia. However, the potential of these samples as genetic resources is currently unassessed. Here, using Sanger and Illumina shotgun sequence data, we explored DNA preservation in specimens of the ground beetle Amara alpina, from both museum and ancient environments. Nearly all museum specimens had amplifiable DNA, with the maximum amplifiable fragment length decreasing with age. Amplification of DNA was only possible in 45% of ancient specimens. Preserved mitochondrial DNA fragments were significantly longer than those of nuclear DNA in both museum and ancient specimens. Metagenomic characterisation of extracted DNA demonstrated that parasite-derived sequences, including Wolbachia and Spiroplasma, are recoverable from museum beetle specimens. Ancient DNA extracts contained beetle DNA in amounts comparable to museum specimens. Overall, our data demonstrate that there is great potential for both museum and ancient beetle specimens in future genetic studies. Reference sequences for museum-1Reference sequences for sample museum-1. Use with file 'Alignment of merged reads for museum-1'.Amara_alpina.reference_list.museum1.fastaReference sequences for museum-2Reference sequences for sample museum-2. Use with file 'Alignment of merged reads for museum-2'.Amara_alpina.reference_list.museum2.fastaReference sequences for modern-1Reference sequences for sample modern-1. Use with file 'Alignment of merged reads for modern-1'.Amara_alpina.reference_list.modern1.fastaReference sequences for modern-2Reference sequences for sample modern-2. Use with file 'Alignment of merged reads for modern-2'.Amara_alpina.reference_list.modern2.fastaReference sequences for ancient-1Reference sequences for sample ancient-1. Use with file 'Alignment of merged reads ... Dataset permafrost Unknown Canada |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
Life sciences medicine and health care metagenomics Late Pleistocene Quaternary Coleoptera Insecta shotgun sequencing Carabidae ancient DNA Recent DNA preservation Wolbachia museum DNA Spiroplasma Holocene Amara alpina Scandinavia North America Russia Canada Sweden archeo museo |
spellingShingle |
Life sciences medicine and health care metagenomics Late Pleistocene Quaternary Coleoptera Insecta shotgun sequencing Carabidae ancient DNA Recent DNA preservation Wolbachia museum DNA Spiroplasma Holocene Amara alpina Scandinavia North America Russia Canada Sweden archeo museo Heintzman, Peter D. Elias, Scott A. Moore, Karen Paszkiewicz, Konrad Barnes, Ian Data from: Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera) |
topic_facet |
Life sciences medicine and health care metagenomics Late Pleistocene Quaternary Coleoptera Insecta shotgun sequencing Carabidae ancient DNA Recent DNA preservation Wolbachia museum DNA Spiroplasma Holocene Amara alpina Scandinavia North America Russia Canada Sweden archeo museo |
description |
DNA preserved in degraded beetle (Coleoptera) specimens, including those derived from dry-stored museum and ancient permafrost-preserved environments, could provide a valuable resource for researchers interested in species and population histories over timescales from decades to millenia. However, the potential of these samples as genetic resources is currently unassessed. Here, using Sanger and Illumina shotgun sequence data, we explored DNA preservation in specimens of the ground beetle Amara alpina, from both museum and ancient environments. Nearly all museum specimens had amplifiable DNA, with the maximum amplifiable fragment length decreasing with age. Amplification of DNA was only possible in 45% of ancient specimens. Preserved mitochondrial DNA fragments were significantly longer than those of nuclear DNA in both museum and ancient specimens. Metagenomic characterisation of extracted DNA demonstrated that parasite-derived sequences, including Wolbachia and Spiroplasma, are recoverable from museum beetle specimens. Ancient DNA extracts contained beetle DNA in amounts comparable to museum specimens. Overall, our data demonstrate that there is great potential for both museum and ancient beetle specimens in future genetic studies. Reference sequences for museum-1Reference sequences for sample museum-1. Use with file 'Alignment of merged reads for museum-1'.Amara_alpina.reference_list.museum1.fastaReference sequences for museum-2Reference sequences for sample museum-2. Use with file 'Alignment of merged reads for museum-2'.Amara_alpina.reference_list.museum2.fastaReference sequences for modern-1Reference sequences for sample modern-1. Use with file 'Alignment of merged reads for modern-1'.Amara_alpina.reference_list.modern1.fastaReference sequences for modern-2Reference sequences for sample modern-2. Use with file 'Alignment of merged reads for modern-2'.Amara_alpina.reference_list.modern2.fastaReference sequences for ancient-1Reference sequences for sample ancient-1. Use with file 'Alignment of merged reads ... |
format |
Dataset |
author |
Heintzman, Peter D. Elias, Scott A. Moore, Karen Paszkiewicz, Konrad Barnes, Ian |
author_facet |
Heintzman, Peter D. Elias, Scott A. Moore, Karen Paszkiewicz, Konrad Barnes, Ian |
author_sort |
Heintzman, Peter D. |
title |
Data from: Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera) |
title_short |
Data from: Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera) |
title_full |
Data from: Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera) |
title_fullStr |
Data from: Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera) |
title_full_unstemmed |
Data from: Characterizing DNA preservation in degraded specimens of Amara alpina (Carabidae: Coleoptera) |
title_sort |
data from: characterizing dna preservation in degraded specimens of amara alpina (carabidae: coleoptera) |
publishDate |
2013 |
url |
https://doi.org/10.5061/dryad.0179t |
geographic |
Canada |
geographic_facet |
Canada |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:84283 10.5061/dryad.0179t oai:easy.dans.knaw.nl:easy-dataset:84283 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
http://dx.doi.org/10.5061/dryad.0179t https://dx.doi.org/10.5061/dryad.0179t |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.0179t |
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1766166837909782528 |