Data from: Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance

• Main Authors: Biederman, Amanda M., Kuhn, Donald E., O'Brien, Kristin M., Crockett, Elizabeth L
• Format: Dataset
• Language: unknown
• Published: 2019
• Subjects: Life sciences; medicine and health care; Antarctic fishes; Membrane fluidity; Notothenia coriiceps; Arrhenius break temperature; Chaenocephalus aceratus; Neuronal membranes; phospholipids; Cholesterol; Western Antarctic Peninsula; envir; socio; Antarctic; Antarctic Peninsula; The Antarctic; Antarc*
• Online Access: https://doi.org/10.5061/dryad.qm0b25h

Disruption of neuronal function is likely to influence limits to thermal tolerance. We hypothesized that with acute warming the structure and function of neuronal membranes in the Antarctic notothenioid fish Chaenocephalus aceratus are more vulnerable to perturbation than membranes in the more thermotolerant notothenioid Notothenia coriiceps. Fluidity was quantified in synaptic membranes, mitochondrial membranes, and myelin from brains of both species of Antarctic fishes. Polar lipid compositions and cholesterol contents were analyzed in myelin; cholesterol was measured in synaptic membranes. Thermal profiles were determined for activities of two membrane-associated proteins, acetylcholinesterase (AChE) and Na+/K+-ATPase (NKA), from brains of animals maintained at ambient temperature or exposed to their critical thermal maxima (CTMAX). Synaptic membranes of C. aceratus were consistently more fluid than those of N. coriiceps (P Biederman et al (2019) AChE activityAcetylcholine esterase activity in brains of Chaenocephalus aceratus and Notothenia coriiceps.Biederman et al (2019) cholesterolCholeresterol in myelin and synaptic membranes from brains of Chaenocephalus aceratus and Notothenia coriiceps.Biederman et al (2019) LDH activityLactate dehydrogenase activity in brains of Chaenocephalus aceratus and Notothenia coriiceps.Biederman et al (2019) marker enzyme enrichmentsMarker enzymes for neuronal membrane preparations in Chaenocephalus aceratus and Notothenia coriiceps.Biederman et al (2019) mitochondrial fluidityFluidity of mitochondrial membranes in brains of Chaenocephalus aceratus and Notothenia coriiceps.Biederman et al (2019) myelin fluidityFluidity of myelin in brains of Chaenocephalus aceratus and Notothenia coriiceps.Biederman et al (2019) NKA activitySodium-potassium ATPase activity in brains of Notothenia coriiceps and Chaenocephalus aceratus.Biederman et al (2019) PK activityPyruvate kinase activity in brains of Notothenia coriiceps and Chaenocephalus aceratus.Biederman et al (2019) polar lipid ...