Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus
Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption (ED) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, Nucella lapi...
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Data Archiving and Networked Services (DANS)
2020
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fttriple:oai:gotriple.eu:50|dedup_wf_001::7019b86c3b4e3265f5303440f7d7f0fe 2023-05-15T18:49:53+02:00 Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon 2020-07-17 https://doi.org/10.5061/dryad.m1063 undefined unknown Data Archiving and Networked Services (DANS) http://dx.doi.org/10.5061/dryad.m1063 https://dx.doi.org/10.5061/dryad.m1063 lic_creative-commons oai:easy.dans.knaw.nl:easy-dataset:82586 10.5061/dryad.m1063 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:82586 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c Life sciences medicine and health care Ecological Genetics Gene Structure and Function Genomics/Proteomics Invertebrates Molecular Evolution Transcriptomics Nucella lapillus psy geo Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2020 fttriple https://doi.org/10.5061/dryad.m1063 2023-01-22T17:23:56Z Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption (ED) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, Nucella lapillus (imposex), caused by organotins, provides one of the most clearcut ecological examples of anthropogenically induced ED in aquatic ecosystems. To identify the underpinning mechanisms of imposex for this ‘nonmodel’ species, we combined Roche 454 pyrosequencing with custom oligoarray fabrication inexpensively to both generate gene models and identify those responding to chronic tributyltin (TBT) treatment. The results supported the involvement of steroid, neuroendocrine peptide hormone dysfunction and retinoid mechanisms, but suggested additionally the involvement of putative peroxisome proliferator–activated receptor (PPAR) pathways. Application of rosiglitazone, a well-known vertebrate PPARγ ligand, to dogwhelks induced imposex in the absence of TBT. Thus, while TBT-induced imposex is linked to the induction of many genes and has a complex phenotype, it is likely also to be driven by PPAR-responsive pathways, hitherto not described in invertebrates. Our findings provide further evidence for a common signalling pathway between invertebrate and vertebrate species that has previously been overlooked in the study of endocrine disruption. Nucella_CoAssemblyCo-assembly data: list of contigs and singletonsSRA021021Control and TBT-exposed libraries: sequencing results Dataset Dogwhelk Nucella lapillus Unknown |
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Open Polar |
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op_collection_id |
fttriple |
language |
unknown |
topic |
Life sciences medicine and health care Ecological Genetics Gene Structure and Function Genomics/Proteomics Invertebrates Molecular Evolution Transcriptomics Nucella lapillus psy geo |
spellingShingle |
Life sciences medicine and health care Ecological Genetics Gene Structure and Function Genomics/Proteomics Invertebrates Molecular Evolution Transcriptomics Nucella lapillus psy geo Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
topic_facet |
Life sciences medicine and health care Ecological Genetics Gene Structure and Function Genomics/Proteomics Invertebrates Molecular Evolution Transcriptomics Nucella lapillus psy geo |
description |
Anthropogenic endocrine disruptors now contaminate all environments globally, with concomitant deleterious effects across diverse taxa. While most studies on endocrine disruption (ED) have focused on vertebrates, the superimposition of male sexual characteristics in the female dogwhelk, Nucella lapillus (imposex), caused by organotins, provides one of the most clearcut ecological examples of anthropogenically induced ED in aquatic ecosystems. To identify the underpinning mechanisms of imposex for this ‘nonmodel’ species, we combined Roche 454 pyrosequencing with custom oligoarray fabrication inexpensively to both generate gene models and identify those responding to chronic tributyltin (TBT) treatment. The results supported the involvement of steroid, neuroendocrine peptide hormone dysfunction and retinoid mechanisms, but suggested additionally the involvement of putative peroxisome proliferator–activated receptor (PPAR) pathways. Application of rosiglitazone, a well-known vertebrate PPARγ ligand, to dogwhelks induced imposex in the absence of TBT. Thus, while TBT-induced imposex is linked to the induction of many genes and has a complex phenotype, it is likely also to be driven by PPAR-responsive pathways, hitherto not described in invertebrates. Our findings provide further evidence for a common signalling pathway between invertebrate and vertebrate species that has previously been overlooked in the study of endocrine disruption. Nucella_CoAssemblyCo-assembly data: list of contigs and singletonsSRA021021Control and TBT-exposed libraries: sequencing results |
format |
Dataset |
author |
Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon |
author_facet |
Pascoal, Sonia Carvalho, Gary Vasieva, Olga Hughes, Roger Cossins, Andrew Fang, Yongxiang Ashelford, Kevin Olohan, Lisa Barroso, Carlos Mendo, Sonia Creer, Simon |
author_sort |
Pascoal, Sonia |
title |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_short |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_full |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_fullStr |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_full_unstemmed |
Data from: Transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, Nucella lapillus |
title_sort |
data from: transcriptomics and in vivo tests reveal novel mechanisms underlying endocrine disruption in an ecological sentinel, nucella lapillus |
publisher |
Data Archiving and Networked Services (DANS) |
publishDate |
2020 |
url |
https://doi.org/10.5061/dryad.m1063 |
genre |
Dogwhelk Nucella lapillus |
genre_facet |
Dogwhelk Nucella lapillus |
op_source |
oai:easy.dans.knaw.nl:easy-dataset:82586 10.5061/dryad.m1063 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:82586 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
http://dx.doi.org/10.5061/dryad.m1063 https://dx.doi.org/10.5061/dryad.m1063 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.m1063 |
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1766243504619520000 |