Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility
DNA sequencing of ancient permafrost samples can be used to reconstruct past plant, animal and bacterial communities. In this study, we assess the small-scale reproducibility of taxonomic composition obtained from sequencing four molecular markers (mitochondrial 12S ribosomal DNA (rDNA), prokaryote...
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Data Archiving and Networked Services (DANS)
2020
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Online Access: | https://doi.org/10.5061/dryad.4m7d1 |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::65e98d77394bcc782abb3467257e7324 2023-05-15T17:57:34+02:00 Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility Porter, Teresita M. Golding, G. Brian King, Christine Froese, Duane Zazula, Grant Poinar, Hendrik N. 2020-06-29 https://doi.org/10.5061/dryad.4m7d1 undefined unknown Data Archiving and Networked Services (DANS) http://dx.doi.org/10.5061/dryad.4m7d1 https://dx.doi.org/10.5061/dryad.4m7d1 lic_creative-commons oai:easy.dans.knaw.nl:easy-dataset:83454 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:83454 10.5061/dryad.4m7d1 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c Life sciences medicine and health care reproducibility Barcoding ancient DNA amplicon pyrosequencing Beringia contamination geo envir Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2020 fttriple https://doi.org/10.5061/dryad.4m7d1 2023-01-22T16:53:31Z DNA sequencing of ancient permafrost samples can be used to reconstruct past plant, animal and bacterial communities. In this study, we assess the small-scale reproducibility of taxonomic composition obtained from sequencing four molecular markers (mitochondrial 12S ribosomal DNA (rDNA), prokaryote 16S rDNA, mitochondrial cox1 and chloroplast trnL intron) from two soil cores sampled 10 cm apart. In addition, sequenced control reactions were used to produce a contaminant library that was used to filter similar sequences from sample libraries. Contaminant filtering resulted in the removal of 1% of reads or 0.3% of operational taxonomic units. We found similar richness, overlap, abundance and taxonomic diversity from the 12S, 16S and trnL markers from each soil core. Jaccard dissimilarity across the two soil cores was highest for metazoan taxa detected by the 12S and cox1 markers. Taxonomic community distances were similar for each marker across the two soil cores when the chi-squared metric was used; however, the 12S and cox1 markers did not cluster well when the Goodall similarity metric was used. A comparison of plant macrofossil vs. read abundance corroborates previous work that suggests eastern Beringia was dominated by grasses and forbs during cold stages of the Pleistocene, a habitat that is restricted to isolated sites in the present-day Yukon. DRYAD_dataflowExcel spreadsheet showing a sample dataflow.finalOTUseedsFasta formatted text files with the seed sequence for each OTU used in the study.perlScriptsPerl scripts used in the study.readidLineageMapTab delimited text files containing the readID for each OTU seed, taxonomic lineage, and OTU abundance.barcodePrimerFilesText files containing the barcodes and primers used in the study.Rscripts[R] scripts used in the study. Dataset permafrost Beringia Yukon Unknown Yukon |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
Life sciences medicine and health care reproducibility Barcoding ancient DNA amplicon pyrosequencing Beringia contamination geo envir |
spellingShingle |
Life sciences medicine and health care reproducibility Barcoding ancient DNA amplicon pyrosequencing Beringia contamination geo envir Porter, Teresita M. Golding, G. Brian King, Christine Froese, Duane Zazula, Grant Poinar, Hendrik N. Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility |
topic_facet |
Life sciences medicine and health care reproducibility Barcoding ancient DNA amplicon pyrosequencing Beringia contamination geo envir |
description |
DNA sequencing of ancient permafrost samples can be used to reconstruct past plant, animal and bacterial communities. In this study, we assess the small-scale reproducibility of taxonomic composition obtained from sequencing four molecular markers (mitochondrial 12S ribosomal DNA (rDNA), prokaryote 16S rDNA, mitochondrial cox1 and chloroplast trnL intron) from two soil cores sampled 10 cm apart. In addition, sequenced control reactions were used to produce a contaminant library that was used to filter similar sequences from sample libraries. Contaminant filtering resulted in the removal of 1% of reads or 0.3% of operational taxonomic units. We found similar richness, overlap, abundance and taxonomic diversity from the 12S, 16S and trnL markers from each soil core. Jaccard dissimilarity across the two soil cores was highest for metazoan taxa detected by the 12S and cox1 markers. Taxonomic community distances were similar for each marker across the two soil cores when the chi-squared metric was used; however, the 12S and cox1 markers did not cluster well when the Goodall similarity metric was used. A comparison of plant macrofossil vs. read abundance corroborates previous work that suggests eastern Beringia was dominated by grasses and forbs during cold stages of the Pleistocene, a habitat that is restricted to isolated sites in the present-day Yukon. DRYAD_dataflowExcel spreadsheet showing a sample dataflow.finalOTUseedsFasta formatted text files with the seed sequence for each OTU used in the study.perlScriptsPerl scripts used in the study.readidLineageMapTab delimited text files containing the readID for each OTU seed, taxonomic lineage, and OTU abundance.barcodePrimerFilesText files containing the barcodes and primers used in the study.Rscripts[R] scripts used in the study. |
format |
Dataset |
author |
Porter, Teresita M. Golding, G. Brian King, Christine Froese, Duane Zazula, Grant Poinar, Hendrik N. |
author_facet |
Porter, Teresita M. Golding, G. Brian King, Christine Froese, Duane Zazula, Grant Poinar, Hendrik N. |
author_sort |
Porter, Teresita M. |
title |
Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility |
title_short |
Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility |
title_full |
Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility |
title_fullStr |
Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility |
title_full_unstemmed |
Data from: Amplicon pyrosequencing late Pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility |
title_sort |
data from: amplicon pyrosequencing late pleistocene permafrost: the removal of putative contaminant sequences and small-scale reproducibility |
publisher |
Data Archiving and Networked Services (DANS) |
publishDate |
2020 |
url |
https://doi.org/10.5061/dryad.4m7d1 |
geographic |
Yukon |
geographic_facet |
Yukon |
genre |
permafrost Beringia Yukon |
genre_facet |
permafrost Beringia Yukon |
op_source |
oai:easy.dans.knaw.nl:easy-dataset:83454 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:83454 10.5061/dryad.4m7d1 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
http://dx.doi.org/10.5061/dryad.4m7d1 https://dx.doi.org/10.5061/dryad.4m7d1 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.4m7d1 |
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1766166028396527616 |