ITS1 versus ITS2 as DNA metabarcodes for fungi
Interactions between plant roots and fungi are well known from most terrestrial ecosystems. Mycorrhizal association is the most prominent plant-fungi interaction, where the fungal partners increase the water and nutrient uptake of their host plants. This symbiosis might be especially important in ma...
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Biotechnology Genetics Ecology Evolution Behavior and Systematics envir geo |
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Biotechnology Genetics Ecology Evolution Behavior and Systematics envir geo Håvard Kauserud Rolf Henrik Nilsson Surendra Kumar Paul M. Kirk Rakel Blaalid Kessy Abarenkov ITS1 versus ITS2 as DNA metabarcodes for fungi |
topic_facet |
Biotechnology Genetics Ecology Evolution Behavior and Systematics envir geo |
description |
Interactions between plant roots and fungi are well known from most terrestrial ecosystems. Mycorrhizal association is the most prominent plant-fungi interaction, where the fungal partners increase the water and nutrient uptake of their host plants. This symbiosis might be especially important in marginal habitats like arctic and alpine environments. The structure, diversity and spatial patterns of the root-associated fungal communities are to a large extent unknown due to previous methodological limitations. The main objective in this thesis was to implement high throughput DNA sequencing to assess the community structure, richness and spatial distribution of root-associated fungal communities in arctic and alpine environments. We focused on one host plant species, namely the ectomycorrhizal herb Bistorta vivipara. Its small and condensed root system enabled us to analyze the entire fungal assemblages associated with individual root systems, using 454 pyrosequencing of ITS1 and/or ITS2 amplicons. All the five studies included in this thesis revealed that the most prominent fungal groups were well-known ectomycorrhizal fungi such as Agaricales, Sebacinales and Thelephorales. Furthermore, ascomycete fungi of the order Helotiales were also recovered frequently across all root systems. Although a high patchiness in fungal community composition generally was observed, some systematic compositional changes along gradients were observed. In a 2x2 m2 local scale study, a spatial autocorrelation was observed at small scales (<0.34 m). Furthermore, a significant compositional difference was observed between the rootassociated fungal communities and adjacent soil fungal communities. Along two primary succession gradients in arctic and alpine areas, a systematic compositional shift was observed. The fungal richness increased along the chronosequences towards the climax vegetation. In a biogeographic survey, where the root-associated fungi were analyzed across Svalbard, a compositional shift was observed that was ... |
format |
Article in Journal/Newspaper |
author |
Håvard Kauserud Rolf Henrik Nilsson Surendra Kumar Paul M. Kirk Rakel Blaalid Kessy Abarenkov |
author_facet |
Håvard Kauserud Rolf Henrik Nilsson Surendra Kumar Paul M. Kirk Rakel Blaalid Kessy Abarenkov |
author_sort |
Håvard Kauserud |
title |
ITS1 versus ITS2 as DNA metabarcodes for fungi |
title_short |
ITS1 versus ITS2 as DNA metabarcodes for fungi |
title_full |
ITS1 versus ITS2 as DNA metabarcodes for fungi |
title_fullStr |
ITS1 versus ITS2 as DNA metabarcodes for fungi |
title_full_unstemmed |
ITS1 versus ITS2 as DNA metabarcodes for fungi |
title_sort |
its1 versus its2 as dna metabarcodes for fungi |
publisher |
Wiley |
publishDate |
2013 |
url |
https://www.duo.uio.no/bitstream/10852/34391/1/dravhandling-blaalid.pdf https://www.mn.uio.no/ibv/english/people/aca/haavarka/blaalid-et-al-2013-mol-ecol-res.pdf https://doi.org/10.1111/1755-0998.12065 http://hdl.handle.net/10852/34391 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12065 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1755-0998.12065 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1755-0998.12065 https://pubmed.ncbi.nlm.nih.gov/23350562/ https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.12065 https://www.ncbi.nlm.nih.gov/pubmed/23350562 https://academic.microsoft.com/#/detail/1995674722 |
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Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
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Arctic Svalbard |
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Arctic Svalbard |
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Molecular Ecology Resources |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::5e5dfbccdaab38ce5e4e88d4c8c7d800 2023-05-15T14:55:46+02:00 ITS1 versus ITS2 as DNA metabarcodes for fungi Håvard Kauserud Rolf Henrik Nilsson Surendra Kumar Paul M. Kirk Rakel Blaalid Kessy Abarenkov 2013-03-01 https://www.duo.uio.no/bitstream/10852/34391/1/dravhandling-blaalid.pdf https://www.mn.uio.no/ibv/english/people/aca/haavarka/blaalid-et-al-2013-mol-ecol-res.pdf https://doi.org/10.1111/1755-0998.12065 http://hdl.handle.net/10852/34391 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12065 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1755-0998.12065 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1755-0998.12065 https://pubmed.ncbi.nlm.nih.gov/23350562/ https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.12065 https://www.ncbi.nlm.nih.gov/pubmed/23350562 https://academic.microsoft.com/#/detail/1995674722 undefined unknown Wiley https://www.duo.uio.no/bitstream/10852/34391/1/dravhandling-blaalid.pdf https://www.mn.uio.no/ibv/english/people/aca/haavarka/blaalid-et-al-2013-mol-ecol-res.pdf http://dx.doi.org/10.1111/1755-0998.12065 https://dx.doi.org/10.1111/1755-0998.12065 http://hdl.handle.net/10852/34391 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12065 https://onlinelibrary.wiley.com/doi/pdf/10.1111/1755-0998.12065 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1755-0998.12065 https://pubmed.ncbi.nlm.nih.gov/23350562/ https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.12065 https://www.ncbi.nlm.nih.gov/pubmed/23350562 https://academic.microsoft.com/#/detail/1995674722 undefined 10.1111/1755-0998.12065 oai:www.duo.uio.no:10852/34391 1995674722 23350562 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|openaire____::55045bd2a65019fd8e6741a755395c8c 10|driver______::4dc196be332447baf11e431bd838e81c 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 10|issn___print::b218938d02cd90e10a9c0a89780b4300 10|openaire____::8ac8380272269217cb09a928c8caa993 10|openaire____::5f532a3fc4f1ea403f37070f59a7a53a 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c 10|openaire____::806360c771262b4d6770e7cdf04b5c5a Biotechnology Genetics Ecology Evolution Behavior and Systematics envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ Thesis https://vocabularies.coar-repositories.org/resource_types/c_46ec/ 2013 fttriple https://doi.org/10.1111/1755-0998.12065 2023-01-22T17:08:33Z Interactions between plant roots and fungi are well known from most terrestrial ecosystems. Mycorrhizal association is the most prominent plant-fungi interaction, where the fungal partners increase the water and nutrient uptake of their host plants. This symbiosis might be especially important in marginal habitats like arctic and alpine environments. The structure, diversity and spatial patterns of the root-associated fungal communities are to a large extent unknown due to previous methodological limitations. The main objective in this thesis was to implement high throughput DNA sequencing to assess the community structure, richness and spatial distribution of root-associated fungal communities in arctic and alpine environments. We focused on one host plant species, namely the ectomycorrhizal herb Bistorta vivipara. Its small and condensed root system enabled us to analyze the entire fungal assemblages associated with individual root systems, using 454 pyrosequencing of ITS1 and/or ITS2 amplicons. All the five studies included in this thesis revealed that the most prominent fungal groups were well-known ectomycorrhizal fungi such as Agaricales, Sebacinales and Thelephorales. Furthermore, ascomycete fungi of the order Helotiales were also recovered frequently across all root systems. Although a high patchiness in fungal community composition generally was observed, some systematic compositional changes along gradients were observed. In a 2x2 m2 local scale study, a spatial autocorrelation was observed at small scales (<0.34 m). Furthermore, a significant compositional difference was observed between the rootassociated fungal communities and adjacent soil fungal communities. Along two primary succession gradients in arctic and alpine areas, a systematic compositional shift was observed. The fungal richness increased along the chronosequences towards the climax vegetation. In a biogeographic survey, where the root-associated fungi were analyzed across Svalbard, a compositional shift was observed that was ... Article in Journal/Newspaper Arctic Svalbard Unknown Arctic Svalbard Molecular Ecology Resources 13 2 218 224 |