Data from: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1
A novel strategy for combating pathogens is through the ongoing development and use of anti-quorum sensing (QS) treatments such as therapeutic bacteria or their anti-QS substances. Relatively little is known about the bacteria that inhabit the open ocean and of their potential anti-pathogenic attrib...
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Online Access: | https://doi.org/10.5061/dryad.27630 |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::5d2e6833d1ebe2484f57d3afc54e266b 2023-05-15T17:35:47+02:00 Data from: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1 Chang, Hong Zhou, Jin Zhu, Xiaoshan Jin, Hui Cai, Zhonghua Chen, Lu Yu, Shenchen 2017-01-01 https://doi.org/10.5061/dryad.27630 undefined unknown http://dx.doi.org/10.5061/dryad.27630 https://dx.doi.org/10.5061/dryad.27630 lic_creative-commons oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:101350 10.5061/dryad.27630 oai:easy.dans.knaw.nl:easy-dataset:101350 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c Life sciences medicine and health care Bacterium Biofilm open ocean Mesozoic Quorum-sensing inhibition Rhizobium sp. NAO1 virulence factors North Altantic Ocean envir socio Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2017 fttriple https://doi.org/10.5061/dryad.27630 2023-01-22T16:52:54Z A novel strategy for combating pathogens is through the ongoing development and use of anti-quorum sensing (QS) treatments such as therapeutic bacteria or their anti-QS substances. Relatively little is known about the bacteria that inhabit the open ocean and of their potential anti-pathogenic attributes; thus, in an initiative to identify these types of therapeutic bacteria, planktonic microbes from the North Atlantic Ocean were collected, isolated, cultured and screened for anti-QS activity. Screening analysis identified one such strain, Rhizobium sp. NAO1. Extracts of Rhizobium sp. NAO1 were identified via ultra-performance liquid chromatography (UPLC) analysis. They were shown to contain N-acyl homoserine lactone (AHL)-based QS analogues (in particular, the N-butyryl homoserine lactone (C4-AHL) analogue) and could disrupt biofilm formation by Pseudomonas aeruginosa PAO1. QS inhibition was confirmed using confocal scanning laser microscopy and growth curves, and it was shown to occur in a dose-dependent manner without affecting bacterial growth. Secondary metabolites of Rhizobium sp. NAO1 inhibited PAO1 pathogenicity by downregulating AHL-mediated virulence factors such as elastase activity and siderophore production. Furthermore, as a result of biofilm structure damage, the secondary metabolite products of Rhizobium sp. NAO1 significantly increased the sensitivity of PAO1 to aminoglycoside antibiotics. Our results demonstrated that Rhizobium sp. strain NAO1 has the ability to disrupt P. aeruginosa PAO1 biofilm architecture, in addition to attenuating P. aeruginosa PAO1 virulence factor production and pathogenicity. Therefore, the newly identified ocean-derived Rhizobium sp. NAO1 has the potential to serve as a QS inhibitor and may be a new microbial resource for drug development. Row Data of Figure 2 to figure 6Row data form figure 2 to figure 6 Dataset North Atlantic Unknown |
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Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
Life sciences medicine and health care Bacterium Biofilm open ocean Mesozoic Quorum-sensing inhibition Rhizobium sp. NAO1 virulence factors North Altantic Ocean envir socio |
spellingShingle |
Life sciences medicine and health care Bacterium Biofilm open ocean Mesozoic Quorum-sensing inhibition Rhizobium sp. NAO1 virulence factors North Altantic Ocean envir socio Chang, Hong Zhou, Jin Zhu, Xiaoshan Jin, Hui Cai, Zhonghua Chen, Lu Yu, Shenchen Data from: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1 |
topic_facet |
Life sciences medicine and health care Bacterium Biofilm open ocean Mesozoic Quorum-sensing inhibition Rhizobium sp. NAO1 virulence factors North Altantic Ocean envir socio |
description |
A novel strategy for combating pathogens is through the ongoing development and use of anti-quorum sensing (QS) treatments such as therapeutic bacteria or their anti-QS substances. Relatively little is known about the bacteria that inhabit the open ocean and of their potential anti-pathogenic attributes; thus, in an initiative to identify these types of therapeutic bacteria, planktonic microbes from the North Atlantic Ocean were collected, isolated, cultured and screened for anti-QS activity. Screening analysis identified one such strain, Rhizobium sp. NAO1. Extracts of Rhizobium sp. NAO1 were identified via ultra-performance liquid chromatography (UPLC) analysis. They were shown to contain N-acyl homoserine lactone (AHL)-based QS analogues (in particular, the N-butyryl homoserine lactone (C4-AHL) analogue) and could disrupt biofilm formation by Pseudomonas aeruginosa PAO1. QS inhibition was confirmed using confocal scanning laser microscopy and growth curves, and it was shown to occur in a dose-dependent manner without affecting bacterial growth. Secondary metabolites of Rhizobium sp. NAO1 inhibited PAO1 pathogenicity by downregulating AHL-mediated virulence factors such as elastase activity and siderophore production. Furthermore, as a result of biofilm structure damage, the secondary metabolite products of Rhizobium sp. NAO1 significantly increased the sensitivity of PAO1 to aminoglycoside antibiotics. Our results demonstrated that Rhizobium sp. strain NAO1 has the ability to disrupt P. aeruginosa PAO1 biofilm architecture, in addition to attenuating P. aeruginosa PAO1 virulence factor production and pathogenicity. Therefore, the newly identified ocean-derived Rhizobium sp. NAO1 has the potential to serve as a QS inhibitor and may be a new microbial resource for drug development. Row Data of Figure 2 to figure 6Row data form figure 2 to figure 6 |
format |
Dataset |
author |
Chang, Hong Zhou, Jin Zhu, Xiaoshan Jin, Hui Cai, Zhonghua Chen, Lu Yu, Shenchen |
author_facet |
Chang, Hong Zhou, Jin Zhu, Xiaoshan Jin, Hui Cai, Zhonghua Chen, Lu Yu, Shenchen |
author_sort |
Chang, Hong |
title |
Data from: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1 |
title_short |
Data from: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1 |
title_full |
Data from: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1 |
title_fullStr |
Data from: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1 |
title_full_unstemmed |
Data from: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1 |
title_sort |
data from: strain identification and quorum sensing inhibition characterization of marine-derived rhizobium sp. nao1 |
publishDate |
2017 |
url |
https://doi.org/10.5061/dryad.27630 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:101350 10.5061/dryad.27630 oai:easy.dans.knaw.nl:easy-dataset:101350 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
http://dx.doi.org/10.5061/dryad.27630 https://dx.doi.org/10.5061/dryad.27630 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.27630 |
_version_ |
1766135055102509056 |