Data from: Cryptic species diversity and reproductive isolation among sympatric lineages of Strongylocentrotus sea urchins in the northwest Atlantic

• Main Authors: Addison, Jason A., Kim, Jin-Hong
• Format: Dataset
• Language: unknown
• Published: Dryad 2018
• Subjects: genetics; mtDNA; Strongylocentrotus droebachiensis; ddRAD; Marine Invertebrate; Recent; Holocene; Strongylocentrotus pallidus; (:tba); Life sciences; medicine and health care; anthro-se; envir; Canada; North Atlantic; Northwest Atlantic
• Online Access: https://doi.org/10.5061/dryad.dn160

Distinguishing between intra- and inter-specific variation in genetic studies is critical to understanding evolution because the mechanisms driving change among populations are expected to be different than those that shape reproductive isolation among lineages. Genetic studies of north Atlantic green sea urchins Strongylocentrotus droebachiensis (Müller, 1776) have detected significant population substructure and asymmetric gene flow from Europe to Atlantic Canada and interspecific hybridization between S. droebachiensis and Strongylocentrotus pallidus (Sars, 1871). However, combined with patterns of divergence at mtDNA sequences, morphological divergence at gamete traits suggests that the European and North American lineages of S. droebachiensis may be cryptic species. Here, we use a combination of cytochrome c oxidase subunit I (COI) sequences and single nucleotide polymorphisms (SNPs) to test for cryptic species within Strongylocentrotus sea urchins and hybrids between S. droebachiensis and S. pallidus populations. We detect striking patterns of habitat and reproductive isolation between two S. droebachiensis lineages, with offshore deep-water collections consisting of S. pallidus in addition to a cryptic lineage sharing genetic similarity with previously published sequences from eastern Atlantic S. droebachiensis. We detected only limited hybridization among all three lineages of sea urchins, suggesting that shared genetic differences previously reported may be a result of historical introgression or incomplete lineage sorting. 110 ASU_supplement dataset_FACETS_09262017Excel spreadsheet with SNP locus info (reference/alternate alleles) and frequency in the GBS sequence data. The second Tab is the STRUCTURE data file of all 3049 SNPs in the 110 individuals included in the study (ID = Depth_IndID_illumina lane)