Data from: Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for el...
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fttriple:oai:gotriple.eu:50|dedup_wf_001::41ab246338486e8c9d0f074e0ff72a87 2023-05-15T13:59:13+02:00 Data from: Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis Tsuji, Masaharu 2020-06-30 https://doi.org/10.5061/dryad.h5v25 undefined unknown Dryad https://dx.doi.org/10.5061/dryad.h5v25 http://dx.doi.org/10.5061/dryad.h5v25 lic_creative-commons 10.5061/dryad.h5v25 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:92688 oai:easy.dans.knaw.nl:easy-dataset:92688 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c metabolite response cold stress basidiomycetous yeast Mrakia blollopis CE-TOFMS Life sciences medicine and health care envir geo Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2020 fttriple https://doi.org/10.5061/dryad.h5v25 2023-01-22T17:23:02Z Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions. Concentration of targeted metabolites data_M. TsujiIn the CE-TOFMS analysis, 219 metabolites (115 cationic, 104 anionic) were detected. Moreover, 88 metabolites, which included amino acids, organic acids, sugar phosphates, and nucleotides, were quantified using external standards and targeted metabolite analysis.MS data set_M. Tsuji.xlsx Dataset Antarc* Antarctic Unknown Antarctic The Antarctic |
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Open Polar |
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topic |
metabolite response cold stress basidiomycetous yeast Mrakia blollopis CE-TOFMS Life sciences medicine and health care envir geo |
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metabolite response cold stress basidiomycetous yeast Mrakia blollopis CE-TOFMS Life sciences medicine and health care envir geo Tsuji, Masaharu Data from: Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
topic_facet |
metabolite response cold stress basidiomycetous yeast Mrakia blollopis CE-TOFMS Life sciences medicine and health care envir geo |
description |
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions. Concentration of targeted metabolites data_M. TsujiIn the CE-TOFMS analysis, 219 metabolites (115 cationic, 104 anionic) were detected. Moreover, 88 metabolites, which included amino acids, organic acids, sugar phosphates, and nucleotides, were quantified using external standards and targeted metabolite analysis.MS data set_M. Tsuji.xlsx |
format |
Dataset |
author |
Tsuji, Masaharu |
author_facet |
Tsuji, Masaharu |
author_sort |
Tsuji, Masaharu |
title |
Data from: Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_short |
Data from: Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_full |
Data from: Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_fullStr |
Data from: Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_full_unstemmed |
Data from: Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
title_sort |
data from: cold-stress responses in the antarctic basidiomycetous yeast mrakia blollopis |
publisher |
Dryad |
publishDate |
2020 |
url |
https://doi.org/10.5061/dryad.h5v25 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
10.5061/dryad.h5v25 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:92688 oai:easy.dans.knaw.nl:easy-dataset:92688 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 re3data_____::r3d100000044 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
https://dx.doi.org/10.5061/dryad.h5v25 http://dx.doi.org/10.5061/dryad.h5v25 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.h5v25 |
_version_ |
1766267724480118784 |