Data from: Real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers
Wolves and dogs provide a paradigmatic example of the ecological and conservation implications of hybridization events between wild and domesticated forms. However, our understanding of such implications has been traditionally hampered by both high genetic similarity and the difficulties in obtainin...
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Data Archiving and Networked Services (DANS)
2014
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Online Access: | https://doi.org/10.5061/dryad.84ph3 |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::3c80cc367dd2110d80480ef9b32de3e6 2023-05-15T15:50:13+02:00 Data from: Real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers Godinho, Raquel López-Bao, José Vicente Castro, Diana Llaneza, Luís Lopes, Susana Silva, Pedro Ferrand, Nuno 2014-01-01 https://doi.org/10.5061/dryad.84ph3 undefined unknown Data Archiving and Networked Services (DANS) http://dx.doi.org/10.5061/dryad.84ph3 https://dx.doi.org/10.5061/dryad.84ph3 lic_creative-commons oai:easy.dans.knaw.nl:easy-dataset:86532 10.5061/dryad.84ph3 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:86532 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c Life sciences medicine and health care microsatellites Canis lupus familiaris wolf simulated genotypes hybridization dog Canis lupus Iberian Peninsula archeo envir Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2014 fttriple https://doi.org/10.5061/dryad.84ph3 2023-01-22T17:23:17Z Wolves and dogs provide a paradigmatic example of the ecological and conservation implications of hybridization events between wild and domesticated forms. However, our understanding of such implications has been traditionally hampered by both high genetic similarity and the difficulties in obtaining tissue samples (TS), which limit our ability to assess ongoing hybridization events. To assess the occurrence and extension of hybridization in a pack of wolf-dog hybrids in Northwestern Iberia, we compared the power of 52 nuclear markers implemented on TS with a subset of 13 ancestry informative markers (AIMs) typed in non-invasive samples (NIS). We demonstrate that the 13 AIMs are as accurate as the 52 markers that were chosen without regard to the power to differentiate between wolves and dogs, also having the advantage of being rapidly screened on NIS. The efficiency of AIMs significantly outperformed ten random sets of similar size and an additional commercial set of 18 markers. Bayesian clustering analysis implemented on AIMs and NIS identified nine hybrids, two wolves and two dogs. Four hybrids were unambiguously assigned to F1xWolf backcrosses. Our approach (AIMs + NIS) overcomes previous difficulties related to sample availability and informative power of markers, allowing a quick identification of wolf-dog hybrids in the first phases of hybridization episodes. This provides managers with a reliable tool to evaluate hybridization, and estimate the success of their actions. This approach may be easily adapted for other pairs of wild/domesticated species, thus improving our understanding of the introgression of domestication genes into natural populations. Genotypes for Iberian Wolves and DogsThis file contains Iberian wolves, dogs and Barbanza individuals’ genotypes. Sheet “52 loci” contains genotypes for the whole set of markers on our dataset. Sheet “13 loci” contains genotypes for the 13 AIMs selected as the most suitable for studying wolfxdog hybridization in this project.Simulated genotypes for wolves ... Dataset Canis lupus Unknown |
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Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
Life sciences medicine and health care microsatellites Canis lupus familiaris wolf simulated genotypes hybridization dog Canis lupus Iberian Peninsula archeo envir |
spellingShingle |
Life sciences medicine and health care microsatellites Canis lupus familiaris wolf simulated genotypes hybridization dog Canis lupus Iberian Peninsula archeo envir Godinho, Raquel López-Bao, José Vicente Castro, Diana Llaneza, Luís Lopes, Susana Silva, Pedro Ferrand, Nuno Data from: Real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers |
topic_facet |
Life sciences medicine and health care microsatellites Canis lupus familiaris wolf simulated genotypes hybridization dog Canis lupus Iberian Peninsula archeo envir |
description |
Wolves and dogs provide a paradigmatic example of the ecological and conservation implications of hybridization events between wild and domesticated forms. However, our understanding of such implications has been traditionally hampered by both high genetic similarity and the difficulties in obtaining tissue samples (TS), which limit our ability to assess ongoing hybridization events. To assess the occurrence and extension of hybridization in a pack of wolf-dog hybrids in Northwestern Iberia, we compared the power of 52 nuclear markers implemented on TS with a subset of 13 ancestry informative markers (AIMs) typed in non-invasive samples (NIS). We demonstrate that the 13 AIMs are as accurate as the 52 markers that were chosen without regard to the power to differentiate between wolves and dogs, also having the advantage of being rapidly screened on NIS. The efficiency of AIMs significantly outperformed ten random sets of similar size and an additional commercial set of 18 markers. Bayesian clustering analysis implemented on AIMs and NIS identified nine hybrids, two wolves and two dogs. Four hybrids were unambiguously assigned to F1xWolf backcrosses. Our approach (AIMs + NIS) overcomes previous difficulties related to sample availability and informative power of markers, allowing a quick identification of wolf-dog hybrids in the first phases of hybridization episodes. This provides managers with a reliable tool to evaluate hybridization, and estimate the success of their actions. This approach may be easily adapted for other pairs of wild/domesticated species, thus improving our understanding of the introgression of domestication genes into natural populations. Genotypes for Iberian Wolves and DogsThis file contains Iberian wolves, dogs and Barbanza individuals’ genotypes. Sheet “52 loci” contains genotypes for the whole set of markers on our dataset. Sheet “13 loci” contains genotypes for the 13 AIMs selected as the most suitable for studying wolfxdog hybridization in this project.Simulated genotypes for wolves ... |
format |
Dataset |
author |
Godinho, Raquel López-Bao, José Vicente Castro, Diana Llaneza, Luís Lopes, Susana Silva, Pedro Ferrand, Nuno |
author_facet |
Godinho, Raquel López-Bao, José Vicente Castro, Diana Llaneza, Luís Lopes, Susana Silva, Pedro Ferrand, Nuno |
author_sort |
Godinho, Raquel |
title |
Data from: Real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers |
title_short |
Data from: Real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers |
title_full |
Data from: Real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers |
title_fullStr |
Data from: Real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers |
title_full_unstemmed |
Data from: Real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers |
title_sort |
data from: real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers |
publisher |
Data Archiving and Networked Services (DANS) |
publishDate |
2014 |
url |
https://doi.org/10.5061/dryad.84ph3 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
oai:easy.dans.knaw.nl:easy-dataset:86532 10.5061/dryad.84ph3 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:86532 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
http://dx.doi.org/10.5061/dryad.84ph3 https://dx.doi.org/10.5061/dryad.84ph3 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.84ph3 |
_version_ |
1766385194571399168 |