Data from: New molecular tools for the identification of two endangered smooth-hound sharks, Mustelus mustelus and Mustelus punctulatus
Mustelus_spp_genotypes.txtMustelus_spp_rawdata The smooth-hounds represent a significant proportion of the elasmobranch catch in the Adriatic basin of the Mediterranean Sea, where the common (Mustelus mustelus) and blackspotted (Mustelus punctulatus) smooth-hounds co-occur. The 2 species share sever...
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fttriple:oai:gotriple.eu:50|dedup_wf_001::32aaa94431491fac364ba9f952e6946e 2023-05-15T17:34:26+02:00 Data from: New molecular tools for the identification of two endangered smooth-hound sharks, Mustelus mustelus and Mustelus punctulatus Marino, Ilaria A. M. Riginella, Emilio Cariani, Alessia Tinti, Fausto Farrell, Edward D. Mazzoldi, Carlotta Zane, Lorenzo 2014-09-04 https://doi.org/10.5061/dryad.39q83 undefined unknown http://dx.doi.org/10.5061/dryad.39q83 https://dx.doi.org/10.5061/dryad.39q83 lic_creative-commons oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:86915 oai:easy.dans.knaw.nl:easy-dataset:86915 10.5061/dryad.39q83 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f re3data_____::r3d100000044 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 Life sciences medicine and health care microsatellite loci Mustelus asterias smooth-hound sharks genetic identification Mitochondrial DNA ITS2 Conservation genetics and biodiversity Mustelus punctulatus Mustelus mustelus Chioggia North Adriatic North Atlantic Bristol Channel envir geo Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2014 fttriple https://doi.org/10.5061/dryad.39q83 2023-01-22T16:52:02Z Mustelus_spp_genotypes.txtMustelus_spp_rawdata The smooth-hounds represent a significant proportion of the elasmobranch catch in the Adriatic basin of the Mediterranean Sea, where the common (Mustelus mustelus) and blackspotted (Mustelus punctulatus) smooth-hounds co-occur. The 2 species share several morphological and morphometric characters that lead to frequent misidentification. In order to provide information useful for their species identification, we performed a morphological identification of several Mustelus specimens to select individuals unambiguously attributed to 1 of the 2 species, and assayed these with 3 new molecular tests. First, we developed and validated a mitochondrial DNA assay based on species-specific amplification of the cytochrome c oxidase subunit 1 (COI). Second, a fragment analysis of 15 microsatellites cross-amplified from several triakid species was performed to identify diagnostic loci. Finally, a length difference was identified in the internal transcribed spacer 2 (ITS2) region and a diagnostic test based on its amplification was established. All the samples classified morphologically as M. mustelus and M. punctulatus showed a species-specific profile using all the 3 molecular tests. In addition, cross-amplification of microsatellites allowed identification of 9 highly polymorphic loci that will be useful for the study of the mating system and population differentiation of the 2 species. Dataset North Atlantic Unknown |
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Open Polar |
collection |
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op_collection_id |
fttriple |
language |
unknown |
topic |
Life sciences medicine and health care microsatellite loci Mustelus asterias smooth-hound sharks genetic identification Mitochondrial DNA ITS2 Conservation genetics and biodiversity Mustelus punctulatus Mustelus mustelus Chioggia North Adriatic North Atlantic Bristol Channel envir geo |
spellingShingle |
Life sciences medicine and health care microsatellite loci Mustelus asterias smooth-hound sharks genetic identification Mitochondrial DNA ITS2 Conservation genetics and biodiversity Mustelus punctulatus Mustelus mustelus Chioggia North Adriatic North Atlantic Bristol Channel envir geo Marino, Ilaria A. M. Riginella, Emilio Cariani, Alessia Tinti, Fausto Farrell, Edward D. Mazzoldi, Carlotta Zane, Lorenzo Data from: New molecular tools for the identification of two endangered smooth-hound sharks, Mustelus mustelus and Mustelus punctulatus |
topic_facet |
Life sciences medicine and health care microsatellite loci Mustelus asterias smooth-hound sharks genetic identification Mitochondrial DNA ITS2 Conservation genetics and biodiversity Mustelus punctulatus Mustelus mustelus Chioggia North Adriatic North Atlantic Bristol Channel envir geo |
description |
Mustelus_spp_genotypes.txtMustelus_spp_rawdata The smooth-hounds represent a significant proportion of the elasmobranch catch in the Adriatic basin of the Mediterranean Sea, where the common (Mustelus mustelus) and blackspotted (Mustelus punctulatus) smooth-hounds co-occur. The 2 species share several morphological and morphometric characters that lead to frequent misidentification. In order to provide information useful for their species identification, we performed a morphological identification of several Mustelus specimens to select individuals unambiguously attributed to 1 of the 2 species, and assayed these with 3 new molecular tests. First, we developed and validated a mitochondrial DNA assay based on species-specific amplification of the cytochrome c oxidase subunit 1 (COI). Second, a fragment analysis of 15 microsatellites cross-amplified from several triakid species was performed to identify diagnostic loci. Finally, a length difference was identified in the internal transcribed spacer 2 (ITS2) region and a diagnostic test based on its amplification was established. All the samples classified morphologically as M. mustelus and M. punctulatus showed a species-specific profile using all the 3 molecular tests. In addition, cross-amplification of microsatellites allowed identification of 9 highly polymorphic loci that will be useful for the study of the mating system and population differentiation of the 2 species. |
format |
Dataset |
author |
Marino, Ilaria A. M. Riginella, Emilio Cariani, Alessia Tinti, Fausto Farrell, Edward D. Mazzoldi, Carlotta Zane, Lorenzo |
author_facet |
Marino, Ilaria A. M. Riginella, Emilio Cariani, Alessia Tinti, Fausto Farrell, Edward D. Mazzoldi, Carlotta Zane, Lorenzo |
author_sort |
Marino, Ilaria A. M. |
title |
Data from: New molecular tools for the identification of two endangered smooth-hound sharks, Mustelus mustelus and Mustelus punctulatus |
title_short |
Data from: New molecular tools for the identification of two endangered smooth-hound sharks, Mustelus mustelus and Mustelus punctulatus |
title_full |
Data from: New molecular tools for the identification of two endangered smooth-hound sharks, Mustelus mustelus and Mustelus punctulatus |
title_fullStr |
Data from: New molecular tools for the identification of two endangered smooth-hound sharks, Mustelus mustelus and Mustelus punctulatus |
title_full_unstemmed |
Data from: New molecular tools for the identification of two endangered smooth-hound sharks, Mustelus mustelus and Mustelus punctulatus |
title_sort |
data from: new molecular tools for the identification of two endangered smooth-hound sharks, mustelus mustelus and mustelus punctulatus |
publishDate |
2014 |
url |
https://doi.org/10.5061/dryad.39q83 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:86915 oai:easy.dans.knaw.nl:easy-dataset:86915 10.5061/dryad.39q83 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f re3data_____::r3d100000044 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 |
op_relation |
http://dx.doi.org/10.5061/dryad.39q83 https://dx.doi.org/10.5061/dryad.39q83 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.39q83 |
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1766133272404819968 |