Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja

• Main Authors: Munro, Catriona, Morris, James P., Brown, Alastair, Hauton, Chris, Thatje, Sven
• Format: Dataset
• Language: unknown
• Published: Dryad Digital Repository 2015
• Subjects: Deep sea; Lithodes maja; species radiation; bottleneck; Evolution; Hydrostatic pressure; Lithodidae; Life sciences; medicine and health care; geo; envir; North Atlantic
• Online Access: https://doi.org/10.5061/dryad.d6t40

Extant deep-sea invertebrate fauna represent both ancient and recent invasions from shallow-water habitats. Hydrostatic pressure may present a significant physiological challenge to organisms seeking to colonize deeper waters or migrate ontogenetically. Pressure may be a key factor contributing to bottlenecks in the radiation of taxa and potentially drive speciation. Here, we assess shifts in the tolerance of hydrostatic pressure through early ontogeny of the northern stone crab Lithodes maja, which occupies a depth range of 4–790 m in the North Atlantic. The zoea I, megalopa and crab I stages were exposed to hydrostatic pressures up to 30.0 MPa (equivalent of 3000 m depth), and the relative fold change of genes putatively coding for the N-methyl-d-aspartate receptor-regulated protein 1 (narg gene), two heat-shock protein 70 kDa (HSP70) isoforms and mitochondrial Citrate Synthase (CS gene) were measured. This study finds a significant increase in the relative expression of the CS and hsp70a genes with increased hydrostatic pressure in the zoea I stage, and an increase in the relative expression of all genes with increased hydrostatic pressure in the megalopa and crab I stages. Transcriptional responses are corroborated by patterns in respiratory rates in response to hydrostatic pressure in all stages. These results suggest a decrease in the acute high-pressure tolerance limit as ontogeny advances, as reflected by a shift in the hydrostatic pressure at which significant differences are observed. Narg FASTA files for alignmentFASTA format files used to derive the narg gene alignmentNarg_FASTA_files_for_alignment.txtClustalO narg alignmentClustal Omega alignment file for the narg geneclustalo_narg_alignment.clustalRpl8 FASTA files for alignmentFASTA files used to derive the rpl8 alignmentRpl8_FASTA_files_for_alignment.txtClustalO rpl8 alignmentClustal Omega download file for rpl8 alignmentclustalo_rpl8_alignment.clustalTubulin FASTA files for alignmentFASTA files used to derives Tubulin gene ...