Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja
Extant deep-sea invertebrate fauna represent both ancient and recent invasions from shallow-water habitats. Hydrostatic pressure may present a significant physiological challenge to organisms seeking to colonize deeper waters or migrate ontogenetically. Pressure may be a key factor contributing to b...
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Online Access: | https://doi.org/10.5061/dryad.d6t40 |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::1b7ac7eeb7d988735474e673d6668ac4 2023-05-15T17:08:06+02:00 Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja Munro, Catriona Morris, James P. Brown, Alastair Hauton, Chris Thatje, Sven 2015-01-01 https://doi.org/10.5061/dryad.d6t40 undefined unknown Dryad Digital Repository https://dx.doi.org/10.5061/dryad.d6t40 http://dx.doi.org/10.5061/dryad.d6t40 lic_creative-commons 10.5061/dryad.d6t40 oai:easy.dans.knaw.nl:easy-dataset:89427 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:89427 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c Deep sea Lithodes maja species radiation bottleneck Evolution Hydrostatic pressure Lithodidae Life sciences medicine and health care geo envir Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2015 fttriple https://doi.org/10.5061/dryad.d6t40 2023-01-22T17:41:48Z Extant deep-sea invertebrate fauna represent both ancient and recent invasions from shallow-water habitats. Hydrostatic pressure may present a significant physiological challenge to organisms seeking to colonize deeper waters or migrate ontogenetically. Pressure may be a key factor contributing to bottlenecks in the radiation of taxa and potentially drive speciation. Here, we assess shifts in the tolerance of hydrostatic pressure through early ontogeny of the northern stone crab Lithodes maja, which occupies a depth range of 4–790 m in the North Atlantic. The zoea I, megalopa and crab I stages were exposed to hydrostatic pressures up to 30.0 MPa (equivalent of 3000 m depth), and the relative fold change of genes putatively coding for the N-methyl-d-aspartate receptor-regulated protein 1 (narg gene), two heat-shock protein 70 kDa (HSP70) isoforms and mitochondrial Citrate Synthase (CS gene) were measured. This study finds a significant increase in the relative expression of the CS and hsp70a genes with increased hydrostatic pressure in the zoea I stage, and an increase in the relative expression of all genes with increased hydrostatic pressure in the megalopa and crab I stages. Transcriptional responses are corroborated by patterns in respiratory rates in response to hydrostatic pressure in all stages. These results suggest a decrease in the acute high-pressure tolerance limit as ontogeny advances, as reflected by a shift in the hydrostatic pressure at which significant differences are observed. Narg FASTA files for alignmentFASTA format files used to derive the narg gene alignmentNarg_FASTA_files_for_alignment.txtClustalO narg alignmentClustal Omega alignment file for the narg geneclustalo_narg_alignment.clustalRpl8 FASTA files for alignmentFASTA files used to derive the rpl8 alignmentRpl8_FASTA_files_for_alignment.txtClustalO rpl8 alignmentClustal Omega download file for rpl8 alignmentclustalo_rpl8_alignment.clustalTubulin FASTA files for alignmentFASTA files used to derives Tubulin gene ... Dataset Lithodes maja North Atlantic Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
Deep sea Lithodes maja species radiation bottleneck Evolution Hydrostatic pressure Lithodidae Life sciences medicine and health care geo envir |
spellingShingle |
Deep sea Lithodes maja species radiation bottleneck Evolution Hydrostatic pressure Lithodidae Life sciences medicine and health care geo envir Munro, Catriona Morris, James P. Brown, Alastair Hauton, Chris Thatje, Sven Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja |
topic_facet |
Deep sea Lithodes maja species radiation bottleneck Evolution Hydrostatic pressure Lithodidae Life sciences medicine and health care geo envir |
description |
Extant deep-sea invertebrate fauna represent both ancient and recent invasions from shallow-water habitats. Hydrostatic pressure may present a significant physiological challenge to organisms seeking to colonize deeper waters or migrate ontogenetically. Pressure may be a key factor contributing to bottlenecks in the radiation of taxa and potentially drive speciation. Here, we assess shifts in the tolerance of hydrostatic pressure through early ontogeny of the northern stone crab Lithodes maja, which occupies a depth range of 4–790 m in the North Atlantic. The zoea I, megalopa and crab I stages were exposed to hydrostatic pressures up to 30.0 MPa (equivalent of 3000 m depth), and the relative fold change of genes putatively coding for the N-methyl-d-aspartate receptor-regulated protein 1 (narg gene), two heat-shock protein 70 kDa (HSP70) isoforms and mitochondrial Citrate Synthase (CS gene) were measured. This study finds a significant increase in the relative expression of the CS and hsp70a genes with increased hydrostatic pressure in the zoea I stage, and an increase in the relative expression of all genes with increased hydrostatic pressure in the megalopa and crab I stages. Transcriptional responses are corroborated by patterns in respiratory rates in response to hydrostatic pressure in all stages. These results suggest a decrease in the acute high-pressure tolerance limit as ontogeny advances, as reflected by a shift in the hydrostatic pressure at which significant differences are observed. Narg FASTA files for alignmentFASTA format files used to derive the narg gene alignmentNarg_FASTA_files_for_alignment.txtClustalO narg alignmentClustal Omega alignment file for the narg geneclustalo_narg_alignment.clustalRpl8 FASTA files for alignmentFASTA files used to derive the rpl8 alignmentRpl8_FASTA_files_for_alignment.txtClustalO rpl8 alignmentClustal Omega download file for rpl8 alignmentclustalo_rpl8_alignment.clustalTubulin FASTA files for alignmentFASTA files used to derives Tubulin gene ... |
format |
Dataset |
author |
Munro, Catriona Morris, James P. Brown, Alastair Hauton, Chris Thatje, Sven |
author_facet |
Munro, Catriona Morris, James P. Brown, Alastair Hauton, Chris Thatje, Sven |
author_sort |
Munro, Catriona |
title |
Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja |
title_short |
Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja |
title_full |
Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja |
title_fullStr |
Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja |
title_full_unstemmed |
Data from: The role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab Lithodes maja |
title_sort |
data from: the role of ontogeny in physiological tolerance: decreasing hydrostatic pressure tolerance with development in the northern stone crab lithodes maja |
publisher |
Dryad Digital Repository |
publishDate |
2015 |
url |
https://doi.org/10.5061/dryad.d6t40 |
genre |
Lithodes maja North Atlantic |
genre_facet |
Lithodes maja North Atlantic |
op_source |
10.5061/dryad.d6t40 oai:easy.dans.knaw.nl:easy-dataset:89427 oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:89427 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c |
op_relation |
https://dx.doi.org/10.5061/dryad.d6t40 http://dx.doi.org/10.5061/dryad.d6t40 |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.d6t40 |
_version_ |
1766063710659411968 |