Data from: Interpreting ELISA analyses from wild animal samples: some recurrent issues and solutions
1. Many studies in disease and immunological ecology rely on the use of assays that quantify the amount of specific antibodies (immunoglobulin) in samples. Enzyme-Linked Immuno Sorbent Assays (ELISAs) are increasingly used in ecology due to their availability for a broad array of antigens and the li...
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2017
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Online Access: | https://doi.org/10.5061/dryad.0qk1h |
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fttriple:oai:gotriple.eu:50|dedup_wf_001::027e17027a75fafeb45ea9d06f6be6ab 2023-05-15T18:07:17+02:00 Data from: Interpreting ELISA analyses from wild animal samples: some recurrent issues and solutions Garnier, Romain Ramos, Raül Sanz-Aguilar, Ana Poisbleau, Maud Weimerskirch, Henri Burthe, Sarah Tornos, Jeremy Boulinier, Thierry 2017-01-01 https://doi.org/10.5061/dryad.0qk1h undefined unknown Dryad Digital Repository https://dx.doi.org/10.5061/dryad.0qk1h http://dx.doi.org/10.5061/dryad.0qk1h lic_creative-commons 10.5061/dryad.0qk1h oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:97950 oai:easy.dans.knaw.nl:easy-dataset:97950 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 disease ecology ELISA immuno-ecology vaccination Borrelia Newcastle Disease Virus seabirds ecological immunology Calonectris borealis Phalacrocorax aristotelis Hydrobates pelagicus melitensis Eudyptes chrysocome Thalassarche carteri Life sciences medicine and health care envir geo Dataset https://vocabularies.coar-repositories.org/resource_types/c_ddb1/ 2017 fttriple https://doi.org/10.5061/dryad.0qk1h 2023-01-22T17:23:02Z 1. Many studies in disease and immunological ecology rely on the use of assays that quantify the amount of specific antibodies (immunoglobulin) in samples. Enzyme-Linked Immuno Sorbent Assays (ELISAs) are increasingly used in ecology due to their availability for a broad array of antigens and the limited amount of sampling material they require. Two recurrent methodological issues are nevertheless faced by researchers: (i) the limited availability of immunological assays and reagents developed for non-model species, and (ii) the statistical determination of the cut-off threshold used to distinguish individual samples that are likely to have or not to have antibodies against a specific antigen. 2. Here, we outline two solutions to deal with these issues. First, we show that implementing two assays with differing detection methods can help validate the use of reagents, such as antibodies, in species different from their intended target. We illustrate this by comparing the quantification of specific vaccinal antibodies against Newcastle Disease Virus (NDV) using two ELISA approaches in four seabird species (Cory’s shearwater, European shag, European storm petrel, and Southern rockhopper penguin). 3. Second, we provide a simple way to determine from the distribution of ELISA values whether the assayed samples are likely to be made of a single group of individuals (likely negative) or of two groups of individuals (negative and positive). We illustrate the use of this approach with two independent datasets: NDV antibody levels following vaccination and anti-Borrelia antibody levels following natural exposure. 4. The practical implementation of these methodological approaches could provide a way to efficiently apply ELISAs and other immune-based assays to address questions in the growing fields of ecological immunology and disease ecology. Data used to compare ELISA techniquesThis file includes all the data used to produce Figure 1. Species are included both as full names and a shorthand used in the provided code. ... Dataset Rockhopper penguin Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
unknown |
topic |
disease ecology ELISA immuno-ecology vaccination Borrelia Newcastle Disease Virus seabirds ecological immunology Calonectris borealis Phalacrocorax aristotelis Hydrobates pelagicus melitensis Eudyptes chrysocome Thalassarche carteri Life sciences medicine and health care envir geo |
spellingShingle |
disease ecology ELISA immuno-ecology vaccination Borrelia Newcastle Disease Virus seabirds ecological immunology Calonectris borealis Phalacrocorax aristotelis Hydrobates pelagicus melitensis Eudyptes chrysocome Thalassarche carteri Life sciences medicine and health care envir geo Garnier, Romain Ramos, Raül Sanz-Aguilar, Ana Poisbleau, Maud Weimerskirch, Henri Burthe, Sarah Tornos, Jeremy Boulinier, Thierry Data from: Interpreting ELISA analyses from wild animal samples: some recurrent issues and solutions |
topic_facet |
disease ecology ELISA immuno-ecology vaccination Borrelia Newcastle Disease Virus seabirds ecological immunology Calonectris borealis Phalacrocorax aristotelis Hydrobates pelagicus melitensis Eudyptes chrysocome Thalassarche carteri Life sciences medicine and health care envir geo |
description |
1. Many studies in disease and immunological ecology rely on the use of assays that quantify the amount of specific antibodies (immunoglobulin) in samples. Enzyme-Linked Immuno Sorbent Assays (ELISAs) are increasingly used in ecology due to their availability for a broad array of antigens and the limited amount of sampling material they require. Two recurrent methodological issues are nevertheless faced by researchers: (i) the limited availability of immunological assays and reagents developed for non-model species, and (ii) the statistical determination of the cut-off threshold used to distinguish individual samples that are likely to have or not to have antibodies against a specific antigen. 2. Here, we outline two solutions to deal with these issues. First, we show that implementing two assays with differing detection methods can help validate the use of reagents, such as antibodies, in species different from their intended target. We illustrate this by comparing the quantification of specific vaccinal antibodies against Newcastle Disease Virus (NDV) using two ELISA approaches in four seabird species (Cory’s shearwater, European shag, European storm petrel, and Southern rockhopper penguin). 3. Second, we provide a simple way to determine from the distribution of ELISA values whether the assayed samples are likely to be made of a single group of individuals (likely negative) or of two groups of individuals (negative and positive). We illustrate the use of this approach with two independent datasets: NDV antibody levels following vaccination and anti-Borrelia antibody levels following natural exposure. 4. The practical implementation of these methodological approaches could provide a way to efficiently apply ELISAs and other immune-based assays to address questions in the growing fields of ecological immunology and disease ecology. Data used to compare ELISA techniquesThis file includes all the data used to produce Figure 1. Species are included both as full names and a shorthand used in the provided code. ... |
format |
Dataset |
author |
Garnier, Romain Ramos, Raül Sanz-Aguilar, Ana Poisbleau, Maud Weimerskirch, Henri Burthe, Sarah Tornos, Jeremy Boulinier, Thierry |
author_facet |
Garnier, Romain Ramos, Raül Sanz-Aguilar, Ana Poisbleau, Maud Weimerskirch, Henri Burthe, Sarah Tornos, Jeremy Boulinier, Thierry |
author_sort |
Garnier, Romain |
title |
Data from: Interpreting ELISA analyses from wild animal samples: some recurrent issues and solutions |
title_short |
Data from: Interpreting ELISA analyses from wild animal samples: some recurrent issues and solutions |
title_full |
Data from: Interpreting ELISA analyses from wild animal samples: some recurrent issues and solutions |
title_fullStr |
Data from: Interpreting ELISA analyses from wild animal samples: some recurrent issues and solutions |
title_full_unstemmed |
Data from: Interpreting ELISA analyses from wild animal samples: some recurrent issues and solutions |
title_sort |
data from: interpreting elisa analyses from wild animal samples: some recurrent issues and solutions |
publisher |
Dryad Digital Repository |
publishDate |
2017 |
url |
https://doi.org/10.5061/dryad.0qk1h |
genre |
Rockhopper penguin |
genre_facet |
Rockhopper penguin |
op_source |
10.5061/dryad.0qk1h oai:services.nod.dans.knaw.nl:Products/dans:oai:easy.dans.knaw.nl:easy-dataset:97950 oai:easy.dans.knaw.nl:easy-dataset:97950 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 re3data_____::r3d100000044 10|eurocrisdris::fe4903425d9040f680d8610d9079ea14 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 10|re3data_____::94816e6421eeb072e7742ce6a9decc5f 10|re3data_____::84e123776089ce3c7a33db98d9cd15a8 |
op_relation |
https://dx.doi.org/10.5061/dryad.0qk1h http://dx.doi.org/10.5061/dryad.0qk1h |
op_rights |
lic_creative-commons |
op_doi |
https://doi.org/10.5061/dryad.0qk1h |
_version_ |
1766179265041137664 |