Effect of acclimatization on hemocyte functional characteristics of the Pacific oyster (Crassostrea gigas) and carpet shell clam (Ruditapes decussatus)
International audience Most experimental procedures on molluscs are done after acclimatization of wild animals to lab conditions. Similarly, short-term acclimation is often unavoidable in a field survey when biological analysis cannot be done within the day of sample collection. However, acclimatiza...
Published in: | Fish & Shellfish Immunology |
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Online Access: | https://doi.org/10.1016/j.fsi.2011.08.016 https://hal.univ-brest.fr/hal-00670314 |
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fttriple:oai:gotriple.eu:10670/1.z0efcb 2023-05-15T15:58:24+02:00 Effect of acclimatization on hemocyte functional characteristics of the Pacific oyster (Crassostrea gigas) and carpet shell clam (Ruditapes decussatus) Hurtado, Miguel, Da Silva, Patricia Mirella Le Goïc, Nelly Palacios, Elena Soudant, Philippe Centro de Investigaciones Biológicas del Noroeste (CIBNOR) centro de Investigaciones Biologicas Del Noroeste Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2011-01-01 https://doi.org/10.1016/j.fsi.2011.08.016 https://hal.univ-brest.fr/hal-00670314 en eng HAL CCSD Elsevier hal-00670314 doi:10.1016/j.fsi.2011.08.016 10670/1.z0efcb https://hal.univ-brest.fr/hal-00670314 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology Fish and Shellfish Immunology, Elsevier, 2011, 31 (6), pp.978-984. ⟨10.1016/j.fsi.2011.08.016⟩ Bivalve Cellular immunity Flow cytometry Phagocytosis ROS production psy envir Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2011 fttriple https://doi.org/10.1016/j.fsi.2011.08.016 2023-01-22T17:56:00Z International audience Most experimental procedures on molluscs are done after acclimatization of wild animals to lab conditions. Similarly, short-term acclimation is often unavoidable in a field survey when biological analysis cannot be done within the day of sample collection. However, acclimatization can affect the general physiological condition and particularly the immune cell responses of molluscs. Our aim was to study the changes in the hemocyte characteristics of the Pacific oyster Crassostrea gigas and the carpet shell clam Ruditapes decussatus acclimated 1 or 2 days under emersed conditions at 14 +/- 1 degrees C and for 1, 2, 7, or 10 days to flowing seawater conditions (submerged) at 9 +/- 1 degrees C, when compared to hemolymph withdrawn from organisms sampled in the field and immediately analyzed in the laboratory (unacclimated). The hemocyte characteristics assessed by flow cytometry were the total (THC) and differential hemocyte count, percentage of dead cells, phagocytosis, and reactive oxygen species (ROS) production. Dead hemocytes were lower in oysters acclimated both in emersed and submerged conditions (1%-5%) compared to those sampled in the field (7%). Compared to oysters, the percentage of dead hemocytes was lower in clams (0.4% vs. 1.1%) and showed a tendency to decrease during acclimatization in both emersed and submerged conditions. In comparison to organisms not acclimated, the phagocytosis of hemocytes decreased in both oysters and clams acclimated under submerged conditions, but was similar in those acclimated in emersed conditions. The ROS production remained stable in both oysters and clams acclimated in emersed conditions, whereas in submerged conditions ROS production did not change in both the hyalinocytes and granulocytes of oysters, but increased in clams. In oysters, the THC decreased when they were acclimated 1 and 2 days in submerged conditions and was mainly caused by a decrease in granulocytes, but the decrease in THC in oysters acclimated 2 days in emersed conditions was ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Unknown Pacific Fish & Shellfish Immunology |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
Bivalve Cellular immunity Flow cytometry Phagocytosis ROS production psy envir |
spellingShingle |
Bivalve Cellular immunity Flow cytometry Phagocytosis ROS production psy envir Hurtado, Miguel, Da Silva, Patricia Mirella Le Goïc, Nelly Palacios, Elena Soudant, Philippe Effect of acclimatization on hemocyte functional characteristics of the Pacific oyster (Crassostrea gigas) and carpet shell clam (Ruditapes decussatus) |
topic_facet |
Bivalve Cellular immunity Flow cytometry Phagocytosis ROS production psy envir |
description |
International audience Most experimental procedures on molluscs are done after acclimatization of wild animals to lab conditions. Similarly, short-term acclimation is often unavoidable in a field survey when biological analysis cannot be done within the day of sample collection. However, acclimatization can affect the general physiological condition and particularly the immune cell responses of molluscs. Our aim was to study the changes in the hemocyte characteristics of the Pacific oyster Crassostrea gigas and the carpet shell clam Ruditapes decussatus acclimated 1 or 2 days under emersed conditions at 14 +/- 1 degrees C and for 1, 2, 7, or 10 days to flowing seawater conditions (submerged) at 9 +/- 1 degrees C, when compared to hemolymph withdrawn from organisms sampled in the field and immediately analyzed in the laboratory (unacclimated). The hemocyte characteristics assessed by flow cytometry were the total (THC) and differential hemocyte count, percentage of dead cells, phagocytosis, and reactive oxygen species (ROS) production. Dead hemocytes were lower in oysters acclimated both in emersed and submerged conditions (1%-5%) compared to those sampled in the field (7%). Compared to oysters, the percentage of dead hemocytes was lower in clams (0.4% vs. 1.1%) and showed a tendency to decrease during acclimatization in both emersed and submerged conditions. In comparison to organisms not acclimated, the phagocytosis of hemocytes decreased in both oysters and clams acclimated under submerged conditions, but was similar in those acclimated in emersed conditions. The ROS production remained stable in both oysters and clams acclimated in emersed conditions, whereas in submerged conditions ROS production did not change in both the hyalinocytes and granulocytes of oysters, but increased in clams. In oysters, the THC decreased when they were acclimated 1 and 2 days in submerged conditions and was mainly caused by a decrease in granulocytes, but the decrease in THC in oysters acclimated 2 days in emersed conditions was ... |
author2 |
Centro de Investigaciones Biológicas del Noroeste (CIBNOR) centro de Investigaciones Biologicas Del Noroeste Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Hurtado, Miguel, Da Silva, Patricia Mirella Le Goïc, Nelly Palacios, Elena Soudant, Philippe |
author_facet |
Hurtado, Miguel, Da Silva, Patricia Mirella Le Goïc, Nelly Palacios, Elena Soudant, Philippe |
author_sort |
Hurtado, Miguel, |
title |
Effect of acclimatization on hemocyte functional characteristics of the Pacific oyster (Crassostrea gigas) and carpet shell clam (Ruditapes decussatus) |
title_short |
Effect of acclimatization on hemocyte functional characteristics of the Pacific oyster (Crassostrea gigas) and carpet shell clam (Ruditapes decussatus) |
title_full |
Effect of acclimatization on hemocyte functional characteristics of the Pacific oyster (Crassostrea gigas) and carpet shell clam (Ruditapes decussatus) |
title_fullStr |
Effect of acclimatization on hemocyte functional characteristics of the Pacific oyster (Crassostrea gigas) and carpet shell clam (Ruditapes decussatus) |
title_full_unstemmed |
Effect of acclimatization on hemocyte functional characteristics of the Pacific oyster (Crassostrea gigas) and carpet shell clam (Ruditapes decussatus) |
title_sort |
effect of acclimatization on hemocyte functional characteristics of the pacific oyster (crassostrea gigas) and carpet shell clam (ruditapes decussatus) |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://doi.org/10.1016/j.fsi.2011.08.016 https://hal.univ-brest.fr/hal-00670314 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1050-4648 EISSN: 1095-9947 Fish and Shellfish Immunology Fish and Shellfish Immunology, Elsevier, 2011, 31 (6), pp.978-984. ⟨10.1016/j.fsi.2011.08.016⟩ |
op_relation |
hal-00670314 doi:10.1016/j.fsi.2011.08.016 10670/1.z0efcb https://hal.univ-brest.fr/hal-00670314 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1016/j.fsi.2011.08.016 |
container_title |
Fish & Shellfish Immunology |
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1766394148927045632 |