Effect of infection by the protistan parasite Marteilia refringens on the enzyme activity and energy reserves of oyster Ostrea stentina (Payraudeau, 1826) in Tunisia

• Published in: Journal of the Marine Biological Association of the United Kingdom
• Main Authors: Elgharsalli, Refka, Seguineau, Catherine, Arzul, Isabelle, Aloui-Béjaoui, Nejla, Quere, Claudie, Moal, Jeanne
• Other Authors: Institut National Agronomique de Tunisie (INAT), Laboratoire de Physiologie des Invertébrés (LPI), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Laboratoire de Génétique et Pathologie (LGP)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2018
• Subjects: ACL; Oysters; energy reserves; pacific oyster; biochemical-composition; new-zealand; crassostrea-gigas thunberg; seasonal-variations; mortality; enzyme activity; host-parasite interaction; Marteilia refringens; ostrea-edulis l; prolonged anoxia; pyruvate-kinase; reproductive activity; envir; socio; New Zealand; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://doi.org/10.1017/S0025315416001156; https://hal.archives-ouvertes.fr/hal-02626099

WOS:000430473000014 This is the first study of the metabolic enzyme activity and energy state of the dwarf oyster Ostrea stentina (Payraudeau, 1826) in Tunisia and in the Mediterranean region. The main purpose of this study was to examine the modulation of the physiological status of the oyster O. stentina depending on the season and the presence of parasite Marteilia refringens. The prevalence of bonamiosis and marteiliosis were established by PCR. Bonamia exitiosa was detected only in 2.91% of oysters while the prevalence of M. refringens was 100% (30 Ind./30) in February and 93.93% (28 Ind./30) in March. A 42-87% mortality rate was reported during the study period. Biochemical analyses were carried out to evaluate the management of the energy resources in regard to the biochemical changes of lipids and carbohydrates. The enzyme activity of pyruvate kinase (PK), citrate synthase (CS) and cytochrome c oxidase (CCO) were measured and the mean level of enzyme activity was respectively 20.31 mU mg(-1) protein; 12.06 mU mg(-1) protein and 3.59 mU mg(-1) protein. Carbohydrate contents of O. stentina were very low all year round with an average of 15.18% in dry weight, and lipid contents remain similar (11.77% in dry weight) compared with the values reported for most other temperate bivalves. Enzyme activity significantly decreased over time (P \textless 0.001). The energy resources of the oyster O. stentina wherein much of the energy was devoted to reproduction seem to affect the defence system.