Differential gene expression analysis in European eels (Anguilla anguilla, L. 1758) naturally infected by macroparasites
We analyzed the relationships between the macro-parasite community of the European eel and the expression of genes involved in the host physiology during its continental life. The genes studied are implicated in: (1) host response to environmental stress, i.e. Heat Shock Protein 70 (HSP70) and metal...
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Online Access: | https://hal.archives-ouvertes.fr/halsde-00293978/file/Fazio-Differential.manuscrit.pdf https://hal.archives-ouvertes.fr/halsde-00293978 |
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fttriple:oai:gotriple.eu:10670/1.xzogk0 2023-05-15T13:28:07+02:00 Differential gene expression analysis in European eels (Anguilla anguilla, L. 1758) naturally infected by macroparasites Fazio, Géraldine, Moné, Hélène, Lecomte-Finiger, Raymonde, Sasal, Pierre Biologie et écologie tropicale et méditerranéenne 2007-2010 (BETM) Université de Perpignan Via Domitia (UPVD)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) 2008-01-01 https://hal.archives-ouvertes.fr/halsde-00293978/file/Fazio-Differential.manuscrit.pdf https://hal.archives-ouvertes.fr/halsde-00293978 en eng HAL CCSD American Society of Parasitologists halsde-00293978 10670/1.xzogk0 https://hal.archives-ouvertes.fr/halsde-00293978/file/Fazio-Differential.manuscrit.pdf https://hal.archives-ouvertes.fr/halsde-00293978 other Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0022-3395 Journal of Parasitology Journal of Parasitology, American Society of Parasitologists, 2008, 94 (3), pp.571-577 envir stat Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2008 fttriple 2023-01-22T18:34:12Z We analyzed the relationships between the macro-parasite community of the European eel and the expression of genes involved in the host physiology during its continental life. The genes studied are implicated in: (1) host response to environmental stress, i.e. Heat Shock Protein 70 (HSP70) and metallothionein (MT); (2) osmoregulation, i.e., ß Thyroid Hormone Receptor (ßTHR) and Na+/K+ATPase; and (3) silvering, i.e., ßTHR, freshwater rod opsin (FWO) and deep-sea rod opsin (DSO). All were enumerated by quantitative RT-PCR. The epizootiological results for 93 yellow eels caught in the Salses- Leucate Lagoon (France) included 11 species, i.e., 1 nematode, 2 acanthocephalans, 1 monogenean, and 7 digeneans. The molecular results revealed: (1) a significant negative relationship between digenean abundance and the expression level of all the tested genes, except FWO; (2) a significant negative relationship between the abundance of the nematode Anguillicola crassus and the expression level of Na+/K+ATPase gene; and (3) a significant positive relationship between the A. crassus abundance and the expression level of MT gene. Eels infected with digeneans had, on average, a lower level of expressed genes. We hypothesize that the parasites may disturb the eel's ability to withstand environmental stress and delay their migration to the Sargasso Sea because of degeneration of the gut. We further propose that the effect of the invasive species, A. crassus, on the gene expression was mainly linked to an increased trophic activity of infected eels. Moreover, it is possible that the parasite may have an effect on the fish's migratory behavior, which is tied to reproductive purposes. Additional work, including an experimental approach, is required to confirm our hypotheses. Article in Journal/Newspaper Anguilla anguilla European eel Unknown |
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envir stat Fazio, Géraldine, Moné, Hélène, Lecomte-Finiger, Raymonde, Sasal, Pierre Differential gene expression analysis in European eels (Anguilla anguilla, L. 1758) naturally infected by macroparasites |
topic_facet |
envir stat |
description |
We analyzed the relationships between the macro-parasite community of the European eel and the expression of genes involved in the host physiology during its continental life. The genes studied are implicated in: (1) host response to environmental stress, i.e. Heat Shock Protein 70 (HSP70) and metallothionein (MT); (2) osmoregulation, i.e., ß Thyroid Hormone Receptor (ßTHR) and Na+/K+ATPase; and (3) silvering, i.e., ßTHR, freshwater rod opsin (FWO) and deep-sea rod opsin (DSO). All were enumerated by quantitative RT-PCR. The epizootiological results for 93 yellow eels caught in the Salses- Leucate Lagoon (France) included 11 species, i.e., 1 nematode, 2 acanthocephalans, 1 monogenean, and 7 digeneans. The molecular results revealed: (1) a significant negative relationship between digenean abundance and the expression level of all the tested genes, except FWO; (2) a significant negative relationship between the abundance of the nematode Anguillicola crassus and the expression level of Na+/K+ATPase gene; and (3) a significant positive relationship between the A. crassus abundance and the expression level of MT gene. Eels infected with digeneans had, on average, a lower level of expressed genes. We hypothesize that the parasites may disturb the eel's ability to withstand environmental stress and delay their migration to the Sargasso Sea because of degeneration of the gut. We further propose that the effect of the invasive species, A. crassus, on the gene expression was mainly linked to an increased trophic activity of infected eels. Moreover, it is possible that the parasite may have an effect on the fish's migratory behavior, which is tied to reproductive purposes. Additional work, including an experimental approach, is required to confirm our hypotheses. |
author2 |
Biologie et écologie tropicale et méditerranéenne 2007-2010 (BETM) Université de Perpignan Via Domitia (UPVD)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Fazio, Géraldine, Moné, Hélène, Lecomte-Finiger, Raymonde, Sasal, Pierre |
author_facet |
Fazio, Géraldine, Moné, Hélène, Lecomte-Finiger, Raymonde, Sasal, Pierre |
author_sort |
Fazio, Géraldine, |
title |
Differential gene expression analysis in European eels (Anguilla anguilla, L. 1758) naturally infected by macroparasites |
title_short |
Differential gene expression analysis in European eels (Anguilla anguilla, L. 1758) naturally infected by macroparasites |
title_full |
Differential gene expression analysis in European eels (Anguilla anguilla, L. 1758) naturally infected by macroparasites |
title_fullStr |
Differential gene expression analysis in European eels (Anguilla anguilla, L. 1758) naturally infected by macroparasites |
title_full_unstemmed |
Differential gene expression analysis in European eels (Anguilla anguilla, L. 1758) naturally infected by macroparasites |
title_sort |
differential gene expression analysis in european eels (anguilla anguilla, l. 1758) naturally infected by macroparasites |
publisher |
HAL CCSD |
publishDate |
2008 |
url |
https://hal.archives-ouvertes.fr/halsde-00293978/file/Fazio-Differential.manuscrit.pdf https://hal.archives-ouvertes.fr/halsde-00293978 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0022-3395 Journal of Parasitology Journal of Parasitology, American Society of Parasitologists, 2008, 94 (3), pp.571-577 |
op_relation |
halsde-00293978 10670/1.xzogk0 https://hal.archives-ouvertes.fr/halsde-00293978/file/Fazio-Differential.manuscrit.pdf https://hal.archives-ouvertes.fr/halsde-00293978 |
op_rights |
other |
_version_ |
1766402312377466880 |