pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas)
WOS:000429100500005 International audience Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and inv...
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2018
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Online Access: | https://doi.org/10.1242/bio.031427 https://hal.archives-ouvertes.fr/hal-02614271/file/Boulais_etal_BO_2018.pdf https://hal.archives-ouvertes.fr/hal-02614271 |
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fttriple:oai:gotriple.eu:10670/1.umtft5 2023-05-15T15:58:05+02:00 pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de microbiologie des environnements extrêmophiles (LM2E) Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) University of South Bohemia ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) 2018-01-01 https://doi.org/10.1242/bio.031427 https://hal.archives-ouvertes.fr/hal-02614271/file/Boulais_etal_BO_2018.pdf https://hal.archives-ouvertes.fr/hal-02614271 en eng HAL CCSD Royal Society hal-02614271 doi:10.1242/bio.031427 10670/1.umtft5 https://hal.archives-ouvertes.fr/hal-02614271/file/Boulais_etal_BO_2018.pdf https://hal.archives-ouvertes.fr/hal-02614271 other Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 2046-6390 Biology Open Biology Open, Royal Society, 2018, 7 (3), pp.UNSP bio031427. ⟨10.1242/bio.031427⟩ ACL marine fish ocean acidification Spermatozoa Motility pecten-maximus respiration pH Salinity metabolism acrosome reaction internal ph intracellular-ph Ions osmolality sea-urchin sperm Seminal fluid envir psy Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2018 fttriple https://doi.org/10.1242/bio.031427 2023-01-22T17:14:39Z WOS:000429100500005 International audience Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82 +/- 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7%(within a range of 0 to 50%), and pH values above 7.5 (within a range of 4.5 to 9.5). Article in Journal/Newspaper Crassostrea gigas Ocean acidification Pacific oyster Unknown Pacific Biology Open |
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Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
ACL marine fish ocean acidification Spermatozoa Motility pecten-maximus respiration pH Salinity metabolism acrosome reaction internal ph intracellular-ph Ions osmolality sea-urchin sperm Seminal fluid envir psy |
spellingShingle |
ACL marine fish ocean acidification Spermatozoa Motility pecten-maximus respiration pH Salinity metabolism acrosome reaction internal ph intracellular-ph Ions osmolality sea-urchin sperm Seminal fluid envir psy Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
topic_facet |
ACL marine fish ocean acidification Spermatozoa Motility pecten-maximus respiration pH Salinity metabolism acrosome reaction internal ph intracellular-ph Ions osmolality sea-urchin sperm Seminal fluid envir psy |
description |
WOS:000429100500005 International audience Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82 +/- 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7%(within a range of 0 to 50%), and pH values above 7.5 (within a range of 4.5 to 9.5). |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de microbiologie des environnements extrêmophiles (LM2E) Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) University of South Bohemia ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011) European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) |
format |
Article in Journal/Newspaper |
author |
Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky |
author_facet |
Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky |
author_sort |
Boulais, Myrina |
title |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_short |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_full |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_fullStr |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_full_unstemmed |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_sort |
ph controls spermatozoa motility in the pacific oyster (crassostrea gigas) |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://doi.org/10.1242/bio.031427 https://hal.archives-ouvertes.fr/hal-02614271/file/Boulais_etal_BO_2018.pdf https://hal.archives-ouvertes.fr/hal-02614271 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Ocean acidification Pacific oyster |
genre_facet |
Crassostrea gigas Ocean acidification Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 2046-6390 Biology Open Biology Open, Royal Society, 2018, 7 (3), pp.UNSP bio031427. ⟨10.1242/bio.031427⟩ |
op_relation |
hal-02614271 doi:10.1242/bio.031427 10670/1.umtft5 https://hal.archives-ouvertes.fr/hal-02614271/file/Boulais_etal_BO_2018.pdf https://hal.archives-ouvertes.fr/hal-02614271 |
op_rights |
other |
op_doi |
https://doi.org/10.1242/bio.031427 |
container_title |
Biology Open |
_version_ |
1766393815296376832 |