Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition

• Published in: Aquaculture Nutrition
• Main Authors: Da Costa, F., Petton, Bruno, Mingant, C., Bougaran, G., Rouxel, C., Quéré, C., Wikfors, G. H., Soudant, Philippe, Robert, R.
• Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique), NOAA National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2016
• Subjects: Tisochrysis lutea; ACL; larvae; lipids; oyster; envir; socio; Crassostrea gigas
• Online Access: https://doi.org/10.1111/anu.12301; https://hal.archives-ouvertes.fr/hal-01483249

International audience Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+ Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93-95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31-33%) and total fatty acids (4.0-4.7 pg cell(-1)). Larval oyster survival and growth rate were positively correlated with 18: 1n-7 and 20: 1n-7, in storage lipids (SL), and negatively related to 14: 0, 18: 1n-9, 20: 1n-9, 20: 4n-6 and trans-22-dehydrocholesterol in membrane lipids (ML). Surprisingly, only the essential fatty acid 20: 5n-3 in SL was correlated positively with larval survival. Correlations suggest that physiological disruption by overabundance of TAG, FFA and certain fatty acids in larvae fed T+ was largely responsible for the poor performance of these larvae. 'High-lipid' strains of microalgae, without regard to qualitative lipid composition, do not always improve bivalve larval performance.