Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides
International audience Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0...
Published in: | Chemical Research in Toxicology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2015
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Online Access: | https://doi.org/10.1021/acs.chemrestox.5b00269 https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 |
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fttriple:oai:gotriple.eu:10670/1.sml6bd 2023-05-15T15:58:02+02:00 Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides Epelboin, Yanouk Quéré, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2015-09-02 https://doi.org/10.1021/acs.chemrestox.5b00269 https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 en eng HAL CCSD American Chemical Society hal-02527813 doi:10.1021/acs.chemrestox.5b00269 10670/1.sml6bd https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0893-228X EISSN: 1520-5010 Chemical Research in Toxicology Chemical Research in Toxicology, American Chemical Society, 2015, 28 (9), pp.1831-1841. ⟨10.1021/acs.chemrestox.5b00269⟩ ACTIVATED PROTEIN-KINASE CRASSOSTREA-GIGAS MOLECULAR-IDENTIFICATION PROTEOMIC RESPONSE OXIDATIVE STRESS CELLULAR-ENERGY PECTEN-MAXIMUS FATTY-ACIDS DIURON PARAMETERS ACL envir socio Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2015 fttriple https://doi.org/10.1021/acs.chemrestox.5b00269 2023-01-22T17:00:38Z International audience Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase alpha (AMPK alpha), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE proteomic revealed that fructose-1,6-bisphosphatase (F-1,6-BP), a key enzyme of gluconeogenesis, was upregulated. Activities of antioxidant enzymes were higher in oysters exposed to the highest pesticide concentrations. Both pesticides enhanced the superoxide dismutase activity of oysters. Isoproturon enhanced catalase activity, and metconazole enhanced peroxiredoxin activity. Overall, our results show that environmental concentrations of metconazole or isoproturon induced subtle changes in the energy and antioxidant metabolisms of oysters. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Unknown Pacific Chemical Research in Toxicology 28 9 1831 1841 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
ACTIVATED PROTEIN-KINASE CRASSOSTREA-GIGAS MOLECULAR-IDENTIFICATION PROTEOMIC RESPONSE OXIDATIVE STRESS CELLULAR-ENERGY PECTEN-MAXIMUS FATTY-ACIDS DIURON PARAMETERS ACL envir socio |
spellingShingle |
ACTIVATED PROTEIN-KINASE CRASSOSTREA-GIGAS MOLECULAR-IDENTIFICATION PROTEOMIC RESPONSE OXIDATIVE STRESS CELLULAR-ENERGY PECTEN-MAXIMUS FATTY-ACIDS DIURON PARAMETERS ACL envir socio Epelboin, Yanouk Quéré, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
topic_facet |
ACTIVATED PROTEIN-KINASE CRASSOSTREA-GIGAS MOLECULAR-IDENTIFICATION PROTEOMIC RESPONSE OXIDATIVE STRESS CELLULAR-ENERGY PECTEN-MAXIMUS FATTY-ACIDS DIURON PARAMETERS ACL envir socio |
description |
International audience Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 mu g/L), isoproturon (0.1 and 1 mu g/L), or both in a mixture (0.2 and 0.1 mu g/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase alpha (AMPK alpha), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE proteomic revealed that fructose-1,6-bisphosphatase (F-1,6-BP), a key enzyme of gluconeogenesis, was upregulated. Activities of antioxidant enzymes were higher in oysters exposed to the highest pesticide concentrations. Both pesticides enhanced the superoxide dismutase activity of oysters. Isoproturon enhanced catalase activity, and metconazole enhanced peroxiredoxin activity. Overall, our results show that environmental concentrations of metconazole or isoproturon induced subtle changes in the energy and antioxidant metabolisms of oysters. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Epelboin, Yanouk Quéré, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte |
author_facet |
Epelboin, Yanouk Quéré, Claudie Pernet, Fabrice Pichereau, Vianney Corporeau, Charlotte |
author_sort |
Epelboin, Yanouk |
title |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_short |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_full |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_fullStr |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_full_unstemmed |
Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides |
title_sort |
energy and antioxidant responses of pacific oyster exposed to trace levels of pesticides |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://doi.org/10.1021/acs.chemrestox.5b00269 https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0893-228X EISSN: 1520-5010 Chemical Research in Toxicology Chemical Research in Toxicology, American Chemical Society, 2015, 28 (9), pp.1831-1841. ⟨10.1021/acs.chemrestox.5b00269⟩ |
op_relation |
hal-02527813 doi:10.1021/acs.chemrestox.5b00269 10670/1.sml6bd https://hal-univ-rennes1.archives-ouvertes.fr/hal-02527813 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1021/acs.chemrestox.5b00269 |
container_title |
Chemical Research in Toxicology |
container_volume |
28 |
container_issue |
9 |
container_start_page |
1831 |
op_container_end_page |
1841 |
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1766393757237772288 |