Culturable diversity of Arctic phytoplankton during pack ice melting
International audience Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied,...
Published in: | Elementa: Science of the Anthropocene |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2020
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Online Access: | https://doi.org/10.1525/elementa.401 https://hal.sorbonne-universite.fr/hal-02557111/file/401-6958-1-PB.pdf https://hal.sorbonne-universite.fr/hal-02557111 |
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fttriple:oai:gotriple.eu:10670/1.s7iizq 2023-05-15T14:45:37+02:00 Culturable diversity of Arctic phytoplankton during pack ice melting Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Le Gall, Florence Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel Universidad Mayor de San Andrés (UMSA) ECOlogy of MArine Plankton (ECOMAP) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Nanyang Technological University Singapour Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) 2020-01-01 https://doi.org/10.1525/elementa.401 https://hal.sorbonne-universite.fr/hal-02557111/file/401-6958-1-PB.pdf https://hal.sorbonne-universite.fr/hal-02557111 en eng HAL CCSD University of California Press hal-02557111 doi:10.1525/elementa.401 10670/1.s7iizq https://hal.sorbonne-universite.fr/hal-02557111/file/401-6958-1-PB.pdf https://hal.sorbonne-universite.fr/hal-02557111 other Hyper Article en Ligne - Sciences de l'Homme et de la Société EISSN: 2325-1026 Elementa: Science of the Anthropocene Elementa: Science of the Anthropocene, University of California Press, 2020, 8 (1), pp.6. ⟨10.1525/elementa.401⟩ Sympagic phytoplankton communities Arctic diatoms Arctic flagellates Isolation techniques 18S rRNA Roscoff culture collection envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2020 fttriple https://doi.org/10.1525/elementa.401 2023-01-22T17:46:17Z International audience Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting, and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy, and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 phylotypes defined by 100% 18S rRNA sequence similarity. These phylotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera Chaetoceros and Attheya. The genera Baffinella and Pyramimonas were the most abundant non-diatom nanoplankton strains, while Micromonas polaris dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera Navicula, Nitzschia, Coscinodiscus, Thalassiosira, Pyramimonas, Mantoniella and Isochrysis. Culturing efforts such as this one highlight the unexplored eukaryotic plankton diversity in the Arctic and provide a large number of strains for analyzing physiological and metabolic impacts in this changing environment. Article in Journal/Newspaper Arctic Baffin Bay Baffin Bay Baffin Phytoplankton Unknown Arctic Baffin Bay Elementa: Science of the Anthropocene 8 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
Sympagic phytoplankton communities Arctic diatoms Arctic flagellates Isolation techniques 18S rRNA Roscoff culture collection envir geo |
spellingShingle |
Sympagic phytoplankton communities Arctic diatoms Arctic flagellates Isolation techniques 18S rRNA Roscoff culture collection envir geo Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Le Gall, Florence Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel Culturable diversity of Arctic phytoplankton during pack ice melting |
topic_facet |
Sympagic phytoplankton communities Arctic diatoms Arctic flagellates Isolation techniques 18S rRNA Roscoff culture collection envir geo |
description |
International audience Massive phytoplankton blooms develop at the Arctic ice edge, sometimes extending far under the pack ice. An extensive culturing effort was conducted before and during a phytoplankton bloom in Baffin Bay between April and July 2016. Different isolation strategies were applied, including flow cytometry cell sorting, manual single cell pipetting, and serial dilution. Although all three techniques yielded the most common organisms, each technique retrieved specific taxa, highlighting the importance of using several methods to maximize the number and diversity of isolated strains. More than 1,000 cultures were obtained, characterized by 18S rRNA sequencing and optical microscopy, and de-replicated to a subset of 276 strains presented in this work. Strains grouped into 57 phylotypes defined by 100% 18S rRNA sequence similarity. These phylotypes spread across five divisions: Heterokontophyta, Chlorophyta, Cryptophyta, Haptophyta and Dinophyta. Diatoms were the most abundant group (193 strains), mostly represented by the genera Chaetoceros and Attheya. The genera Baffinella and Pyramimonas were the most abundant non-diatom nanoplankton strains, while Micromonas polaris dominated the picoplankton. Diversity at the class level was higher during the peak of the bloom. Potentially new species were isolated, in particular within the genera Navicula, Nitzschia, Coscinodiscus, Thalassiosira, Pyramimonas, Mantoniella and Isochrysis. Culturing efforts such as this one highlight the unexplored eukaryotic plankton diversity in the Arctic and provide a large number of strains for analyzing physiological and metabolic impacts in this changing environment. |
author2 |
Universidad Mayor de San Andrés (UMSA) ECOlogy of MArine Plankton (ECOMAP) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Nanyang Technological University Singapour Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Le Gall, Florence Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel |
author_facet |
Ribeiro, Catherine Gérikas dos Santos, Adriana Lopes Gourvil, Priscillia Le Gall, Florence Marie, Dominique Tragin, Margot Probert, Ian Vaulot, Daniel |
author_sort |
Ribeiro, Catherine Gérikas |
title |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_short |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_full |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_fullStr |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_full_unstemmed |
Culturable diversity of Arctic phytoplankton during pack ice melting |
title_sort |
culturable diversity of arctic phytoplankton during pack ice melting |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://doi.org/10.1525/elementa.401 https://hal.sorbonne-universite.fr/hal-02557111/file/401-6958-1-PB.pdf https://hal.sorbonne-universite.fr/hal-02557111 |
geographic |
Arctic Baffin Bay |
geographic_facet |
Arctic Baffin Bay |
genre |
Arctic Baffin Bay Baffin Bay Baffin Phytoplankton |
genre_facet |
Arctic Baffin Bay Baffin Bay Baffin Phytoplankton |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société EISSN: 2325-1026 Elementa: Science of the Anthropocene Elementa: Science of the Anthropocene, University of California Press, 2020, 8 (1), pp.6. ⟨10.1525/elementa.401⟩ |
op_relation |
hal-02557111 doi:10.1525/elementa.401 10670/1.s7iizq https://hal.sorbonne-universite.fr/hal-02557111/file/401-6958-1-PB.pdf https://hal.sorbonne-universite.fr/hal-02557111 |
op_rights |
other |
op_doi |
https://doi.org/10.1525/elementa.401 |
container_title |
Elementa: Science of the Anthropocene |
container_volume |
8 |
_version_ |
1766316997778341888 |