Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov.
Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Usin...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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Microbiology Society
2021
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Online Access: | https://doi.org/10.1099/ijsem.0.004654 https://archimer.ifremer.fr/doc/00677/78893/81195.pdf https://archimer.ifremer.fr/doc/00677/78893/81196.pdf https://archimer.ifremer.fr/doc/00677/78893/ |
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fttriple:oai:gotriple.eu:10670/1.qix5y2 2023-05-15T17:54:20+02:00 Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. Garcia, Celine Mesnil, Aurelie Tourbiez, Delphine Moussa Pouly, Mirna Dubreuil, Christine Gonçalves De Sa, Amélie Chollet, Bruno Godfrin, Yoann Dégremont, Lionel Serpin, Delphine Travers, Marie-agnes 2021-01-01 https://doi.org/10.1099/ijsem.0.004654 https://archimer.ifremer.fr/doc/00677/78893/81195.pdf https://archimer.ifremer.fr/doc/00677/78893/81196.pdf https://archimer.ifremer.fr/doc/00677/78893/ en eng Microbiology Society doi:10.1099/ijsem.0.004654 10670/1.qix5y2 https://archimer.ifremer.fr/doc/00677/78893/81195.pdf https://archimer.ifremer.fr/doc/00677/78893/81196.pdf https://archimer.ifremer.fr/doc/00677/78893/ other Archimer, archive institutionnelle de l'Ifremer International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Microbiology Society), 2021 , Vol. 71 , N. 2 , P. 004654 (10p.) envir geo Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ 2021 fttriple https://doi.org/10.1099/ijsem.0.004654 2023-01-22T16:46:39Z Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Using phenotypic analysis combined with DNA–DNA hybridization (DDH) and whole genome sequencing, the isolates were shown to belong to Vibrio aestuarianus , a species regularly detected in France during oyster mortality events. Comparison of the strains from cockles with strains from French oysters and the type strain showed that the strains from cockles were genetically different to those from oysters and also different to the V. aestuarianus type strain. Moreover, the cockle and oyster strains were classified into two different, but close, groups both separated from the type strain by: (1) analyses of the ldh gene sequences; (2) DDH assays between 12/122 3T3T (LMG 31436T=DSM 109723T), a representative cockle strain, 02/041T (CIP 109791T=LMG 24517T) representative oyster strain and V. aestuarianus type strain LMG 7909T; (3) average nucleotide identity values calculated on the genomes; and (4) phenotypic traits. Finally, results of MALDI-TOF analyses also revealed specific peaks discriminating the three representative strains. The toxicity of representative strains of these cockle isolates was demonstrated by experimental infection of hatchery-produced cockles. The data therefore allow us to propose two novel subspecies of Vibrio aestuarianus : Vibrio aestuarianus subsp. cardii subsp. nov. for the cockle strains and Vibrio aestuarianus subsp. francensis subsp. nov. for the Pacific oyster strains, in addition to an emended description of the species Vibrio aestuarianus . Text Pacific oyster Unknown Pacific International Journal of Systematic and Evolutionary Microbiology 71 2 |
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envir geo |
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envir geo Garcia, Celine Mesnil, Aurelie Tourbiez, Delphine Moussa Pouly, Mirna Dubreuil, Christine Gonçalves De Sa, Amélie Chollet, Bruno Godfrin, Yoann Dégremont, Lionel Serpin, Delphine Travers, Marie-agnes Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
topic_facet |
envir geo |
description |
Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Using phenotypic analysis combined with DNA–DNA hybridization (DDH) and whole genome sequencing, the isolates were shown to belong to Vibrio aestuarianus , a species regularly detected in France during oyster mortality events. Comparison of the strains from cockles with strains from French oysters and the type strain showed that the strains from cockles were genetically different to those from oysters and also different to the V. aestuarianus type strain. Moreover, the cockle and oyster strains were classified into two different, but close, groups both separated from the type strain by: (1) analyses of the ldh gene sequences; (2) DDH assays between 12/122 3T3T (LMG 31436T=DSM 109723T), a representative cockle strain, 02/041T (CIP 109791T=LMG 24517T) representative oyster strain and V. aestuarianus type strain LMG 7909T; (3) average nucleotide identity values calculated on the genomes; and (4) phenotypic traits. Finally, results of MALDI-TOF analyses also revealed specific peaks discriminating the three representative strains. The toxicity of representative strains of these cockle isolates was demonstrated by experimental infection of hatchery-produced cockles. The data therefore allow us to propose two novel subspecies of Vibrio aestuarianus : Vibrio aestuarianus subsp. cardii subsp. nov. for the cockle strains and Vibrio aestuarianus subsp. francensis subsp. nov. for the Pacific oyster strains, in addition to an emended description of the species Vibrio aestuarianus . |
format |
Text |
author |
Garcia, Celine Mesnil, Aurelie Tourbiez, Delphine Moussa Pouly, Mirna Dubreuil, Christine Gonçalves De Sa, Amélie Chollet, Bruno Godfrin, Yoann Dégremont, Lionel Serpin, Delphine Travers, Marie-agnes |
author_facet |
Garcia, Celine Mesnil, Aurelie Tourbiez, Delphine Moussa Pouly, Mirna Dubreuil, Christine Gonçalves De Sa, Amélie Chollet, Bruno Godfrin, Yoann Dégremont, Lionel Serpin, Delphine Travers, Marie-agnes |
author_sort |
Garcia, Celine |
title |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_short |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_full |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_fullStr |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_full_unstemmed |
Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov. |
title_sort |
vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles cerastoderma edule in france, and establishment of vibrio aestuarianus subsp. aestuarianus subsp. nov. and vibrio aestuarianus subsp. francensis subsp. nov. |
publisher |
Microbiology Society |
publishDate |
2021 |
url |
https://doi.org/10.1099/ijsem.0.004654 https://archimer.ifremer.fr/doc/00677/78893/81195.pdf https://archimer.ifremer.fr/doc/00677/78893/81196.pdf https://archimer.ifremer.fr/doc/00677/78893/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Archimer, archive institutionnelle de l'Ifremer International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Microbiology Society), 2021 , Vol. 71 , N. 2 , P. 004654 (10p.) |
op_relation |
doi:10.1099/ijsem.0.004654 10670/1.qix5y2 https://archimer.ifremer.fr/doc/00677/78893/81195.pdf https://archimer.ifremer.fr/doc/00677/78893/81196.pdf https://archimer.ifremer.fr/doc/00677/78893/ |
op_rights |
other |
op_doi |
https://doi.org/10.1099/ijsem.0.004654 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
71 |
container_issue |
2 |
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1766162092860112896 |