Transcriptomic Profiling of Gametogenesis in Triploid Pacific Oysters Crassostrea gigas: Towards an Understanding of Partial Sterility Associated with Triploidy
International audience Background: Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named ''3nb...
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Online Access: | https://doi.org/10.1371/journal.pone.0112094 https://hal.sorbonne-universite.fr/hal-01345129 |
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fttriple:oai:gotriple.eu:10670/1.qiiju9 2023-05-15T15:58:14+02:00 Transcriptomic Profiling of Gametogenesis in Triploid Pacific Oysters Crassostrea gigas: Towards an Understanding of Partial Sterility Associated with Triploidy Dheilly, Nolwenn, Jouaux, Aude Boudry, Pierre Favrel, Pascal Lelong, Christophe Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2014-11-06 https://doi.org/10.1371/journal.pone.0112094 https://hal.sorbonne-universite.fr/hal-01345129 en eng HAL CCSD Public Library of Science hal-01345129 doi:10.1371/journal.pone.0112094 10670/1.qiiju9 https://hal.sorbonne-universite.fr/hal-01345129 lic_creative-commons other Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE PLoS ONE, Public Library of Science, 2014, 9 (11), pp.e112094. ⟨10.1371/journal.pone.0112094⟩ ACL PERFORMANCE TEMPERATURE IDENTIFICATION DIFFERENTIATION SPERMATOGENESIS SEX GENE-EXPRESSION THUNBERG GROWTH FISH envir droit Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2014 fttriple https://doi.org/10.1371/journal.pone.0112094 2023-01-22T18:29:58Z International audience Background: Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named ''3nb'') yet a low but significant proportion show an advanced gametogenesis (named ''3na''). These oysters thus constitute an interesting model to study the effect of triploidy on germ cell development. We used microarrays to compare the gonad transcriptomes of diploid 2n and the abovementioned triploid 3nb and 3na male and female oysters throughout gametogenesis. Results: All triploids displayed an upregulation of genes related to DNA repair and apoptosis and a downregulation of genes associated with cell division. The comparison of 3na and 3nb transcriptomes with 2n revealed the likely involvement of a cell cycle checkpoint during mitosis in the successful but delayed development of gonads in 3na individuals. In contrast, a disruption of sex differentiation mechanisms may explain the sterility of 3nb individuals with 3nb females expressing male-specific genes and 3nb males expressing female-specific genes. Conclusions: The disruption of sex differentiation and mitosis may be responsible for the impaired gametogenesis of triploid Pacific oysters. The function of the numerous candidate genes identified in our study should now be studied in detail in order to elucidate their role in sex determination, mitosis/meiosis control, pachytene cell cycle checkpoint, and the control of DNA repair/apoptosis. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Unknown Pacific PLoS ONE 9 11 e112094 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
ACL PERFORMANCE TEMPERATURE IDENTIFICATION DIFFERENTIATION SPERMATOGENESIS SEX GENE-EXPRESSION THUNBERG GROWTH FISH envir droit |
spellingShingle |
ACL PERFORMANCE TEMPERATURE IDENTIFICATION DIFFERENTIATION SPERMATOGENESIS SEX GENE-EXPRESSION THUNBERG GROWTH FISH envir droit Dheilly, Nolwenn, Jouaux, Aude Boudry, Pierre Favrel, Pascal Lelong, Christophe Transcriptomic Profiling of Gametogenesis in Triploid Pacific Oysters Crassostrea gigas: Towards an Understanding of Partial Sterility Associated with Triploidy |
topic_facet |
ACL PERFORMANCE TEMPERATURE IDENTIFICATION DIFFERENTIATION SPERMATOGENESIS SEX GENE-EXPRESSION THUNBERG GROWTH FISH envir droit |
description |
International audience Background: Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named ''3nb'') yet a low but significant proportion show an advanced gametogenesis (named ''3na''). These oysters thus constitute an interesting model to study the effect of triploidy on germ cell development. We used microarrays to compare the gonad transcriptomes of diploid 2n and the abovementioned triploid 3nb and 3na male and female oysters throughout gametogenesis. Results: All triploids displayed an upregulation of genes related to DNA repair and apoptosis and a downregulation of genes associated with cell division. The comparison of 3na and 3nb transcriptomes with 2n revealed the likely involvement of a cell cycle checkpoint during mitosis in the successful but delayed development of gonads in 3na individuals. In contrast, a disruption of sex differentiation mechanisms may explain the sterility of 3nb individuals with 3nb females expressing male-specific genes and 3nb males expressing female-specific genes. Conclusions: The disruption of sex differentiation and mitosis may be responsible for the impaired gametogenesis of triploid Pacific oysters. The function of the numerous candidate genes identified in our study should now be studied in detail in order to elucidate their role in sex determination, mitosis/meiosis control, pachytene cell cycle checkpoint, and the control of DNA repair/apoptosis. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Dheilly, Nolwenn, Jouaux, Aude Boudry, Pierre Favrel, Pascal Lelong, Christophe |
author_facet |
Dheilly, Nolwenn, Jouaux, Aude Boudry, Pierre Favrel, Pascal Lelong, Christophe |
author_sort |
Dheilly, Nolwenn, |
title |
Transcriptomic Profiling of Gametogenesis in Triploid Pacific Oysters Crassostrea gigas: Towards an Understanding of Partial Sterility Associated with Triploidy |
title_short |
Transcriptomic Profiling of Gametogenesis in Triploid Pacific Oysters Crassostrea gigas: Towards an Understanding of Partial Sterility Associated with Triploidy |
title_full |
Transcriptomic Profiling of Gametogenesis in Triploid Pacific Oysters Crassostrea gigas: Towards an Understanding of Partial Sterility Associated with Triploidy |
title_fullStr |
Transcriptomic Profiling of Gametogenesis in Triploid Pacific Oysters Crassostrea gigas: Towards an Understanding of Partial Sterility Associated with Triploidy |
title_full_unstemmed |
Transcriptomic Profiling of Gametogenesis in Triploid Pacific Oysters Crassostrea gigas: Towards an Understanding of Partial Sterility Associated with Triploidy |
title_sort |
transcriptomic profiling of gametogenesis in triploid pacific oysters crassostrea gigas: towards an understanding of partial sterility associated with triploidy |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pone.0112094 https://hal.sorbonne-universite.fr/hal-01345129 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE PLoS ONE, Public Library of Science, 2014, 9 (11), pp.e112094. ⟨10.1371/journal.pone.0112094⟩ |
op_relation |
hal-01345129 doi:10.1371/journal.pone.0112094 10670/1.qiiju9 https://hal.sorbonne-universite.fr/hal-01345129 |
op_rights |
lic_creative-commons other |
op_doi |
https://doi.org/10.1371/journal.pone.0112094 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
11 |
container_start_page |
e112094 |
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1766393965675806720 |