Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios
International audience Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to i...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2019
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2019.02067 https://hal.sorbonne-universite.fr/hal-02298362/file/fmicb-10-02067.pdf https://hal.sorbonne-universite.fr/hal-02298362 |
id |
fttriple:oai:gotriple.eu:10670/1.qf5yhw |
---|---|
record_format |
openpolar |
spelling |
fttriple:oai:gotriple.eu:10670/1.qf5yhw 2023-05-15T17:54:19+02:00 Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios Wegner, K. Mathias Piel, Damien Bruto, Maxime John, Uwe Mao, Zhijuan Alunno-Bruscia, Marianne Petton, Bruno Le Roux, Frédérique Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung (AWI) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Zhejiang Normal University Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) ANR-16-CE32-0008,REVENGE,L'huître comme niche de l'évolution et l'émergence de vibrios pathogènes(2016) 2019-09-06 https://doi.org/10.3389/fmicb.2019.02067 https://hal.sorbonne-universite.fr/hal-02298362/file/fmicb-10-02067.pdf https://hal.sorbonne-universite.fr/hal-02298362 en eng HAL CCSD Frontiers Media hal-02298362 doi:10.3389/fmicb.2019.02067 10670/1.qf5yhw https://hal.sorbonne-universite.fr/hal-02298362/file/fmicb-10-02067.pdf https://hal.sorbonne-universite.fr/hal-02298362 other Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2019, 10, pp.2067. ⟨10.3389/fmicb.2019.02067⟩ ACL host-pathogen interaction Wadden Sea emerging disease biological invasion local adaptation virulence envir scipo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2019 fttriple https://doi.org/10.3389/fmicb.2019.02067 2023-01-22T18:22:57Z International audience Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between different host populations and their locally encountered Vibrio communities. Here, we explore the molecular targets of such co-evolutionary interactions by analyzing the genomes of nine Vibrio strains from the Splendidus-clade showing opposite virulence patterns towards two populations of Pacific oysters introduced into European Wadden Sea. By contrasting Vibrio phylogeny to their host specific virulence patterns, we could identify two core genome genes (OG1907 and OG 3159) that determine the genotype by genotype (G × G) interactions between oyster larvae and their sympatric Vibrio communities. Both genes show positive selection between locations targeting only few amino acid positions. Deletion of each gene led to a loss of the host specific virulence patterns while complementation with OG3159 alleles from both locations could recreate the wild type phenotypes matching the origin of the allele. This indicates that both genes can act as a genetic switch for Vibrio-oyster coevolution demonstrating that local adaptation in distinct Vibrio lineages can rely on only few genes independent of larger pathogenicity islands or plasmids. Article in Journal/Newspaper Pacific oyster Unknown Pacific Frontiers in Microbiology 10 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
ACL host-pathogen interaction Wadden Sea emerging disease biological invasion local adaptation virulence envir scipo |
spellingShingle |
ACL host-pathogen interaction Wadden Sea emerging disease biological invasion local adaptation virulence envir scipo Wegner, K. Mathias Piel, Damien Bruto, Maxime John, Uwe Mao, Zhijuan Alunno-Bruscia, Marianne Petton, Bruno Le Roux, Frédérique Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios |
topic_facet |
ACL host-pathogen interaction Wadden Sea emerging disease biological invasion local adaptation virulence envir scipo |
description |
International audience Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between different host populations and their locally encountered Vibrio communities. Here, we explore the molecular targets of such co-evolutionary interactions by analyzing the genomes of nine Vibrio strains from the Splendidus-clade showing opposite virulence patterns towards two populations of Pacific oysters introduced into European Wadden Sea. By contrasting Vibrio phylogeny to their host specific virulence patterns, we could identify two core genome genes (OG1907 and OG 3159) that determine the genotype by genotype (G × G) interactions between oyster larvae and their sympatric Vibrio communities. Both genes show positive selection between locations targeting only few amino acid positions. Deletion of each gene led to a loss of the host specific virulence patterns while complementation with OG3159 alleles from both locations could recreate the wild type phenotypes matching the origin of the allele. This indicates that both genes can act as a genetic switch for Vibrio-oyster coevolution demonstrating that local adaptation in distinct Vibrio lineages can rely on only few genes independent of larger pathogenicity islands or plasmids. |
author2 |
Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung (AWI) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Zhejiang Normal University Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) ANR-16-CE32-0008,REVENGE,L'huître comme niche de l'évolution et l'émergence de vibrios pathogènes(2016) |
format |
Article in Journal/Newspaper |
author |
Wegner, K. Mathias Piel, Damien Bruto, Maxime John, Uwe Mao, Zhijuan Alunno-Bruscia, Marianne Petton, Bruno Le Roux, Frédérique |
author_facet |
Wegner, K. Mathias Piel, Damien Bruto, Maxime John, Uwe Mao, Zhijuan Alunno-Bruscia, Marianne Petton, Bruno Le Roux, Frédérique |
author_sort |
Wegner, K. Mathias |
title |
Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios |
title_short |
Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios |
title_full |
Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios |
title_fullStr |
Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios |
title_full_unstemmed |
Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios |
title_sort |
molecular targets for coevolutionary interactions between pacific oyster larvae and their sympatric vibrios |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://doi.org/10.3389/fmicb.2019.02067 https://hal.sorbonne-universite.fr/hal-02298362/file/fmicb-10-02067.pdf https://hal.sorbonne-universite.fr/hal-02298362 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1664-302X EISSN: 1664-302X Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2019, 10, pp.2067. ⟨10.3389/fmicb.2019.02067⟩ |
op_relation |
hal-02298362 doi:10.3389/fmicb.2019.02067 10670/1.qf5yhw https://hal.sorbonne-universite.fr/hal-02298362/file/fmicb-10-02067.pdf https://hal.sorbonne-universite.fr/hal-02298362 |
op_rights |
other |
op_doi |
https://doi.org/10.3389/fmicb.2019.02067 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
_version_ |
1766162067841089536 |