Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas
00000 ăWOS:000383370300057 International audience This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS wit...
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Online Access: | https://doi.org/10.1016/j.aquaculture.2016.07.020 https://hal.archives-ouvertes.fr/hal-01483196 |
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fttriple:oai:gotriple.eu:10670/1.qd75r2 2023-05-15T15:58:32+02:00 Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas Asmani, Katia Petton, Bruno Le Grand, Jacqueline Mounier, Jérôme Robert, Rene Nicolas, Jean-Louis Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) Université de Brest (UBO) IFR148 ScInBioS European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) 2016-11-01 https://doi.org/10.1016/j.aquaculture.2016.07.020 https://hal.archives-ouvertes.fr/hal-01483196 en eng HAL CCSD Elsevier hal-01483196 doi:10.1016/j.aquaculture.2016.07.020 10670/1.qd75r2 https://hal.archives-ouvertes.fr/hal-01483196 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0044-8486 Aquaculture Aquaculture, Elsevier, 2016, 464, pp.434-444. ⟨10.1016/j.aquaculture.2016.07.020⟩ systems ras recirculation aquaculture bacterial diversity Bivalve larvae communities dynamics hatchery marine bivalves Microbiota Pyrosequencing temperature vibrio-parahaemolyticus ACL envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2016 fttriple https://doi.org/10.1016/j.aquaculture.2016.07.020 2023-01-22T17:56:39Z 00000 ăWOS:000383370300057 International audience This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5% in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 10(5) ml(-1)) and in larvae (10(3) larva(-1)) on day 15. Bacterial assemblages, characterised by 454 pyrosequencing of the V1-V3 region of 16S rRNA, were highly similar (50-65%) for compartments, regardless of rearing system and sampling time, but the compartments were clearly different from one another. At the beginning of rearing, larval microbiota was mostly composed of Proteobacteria (similar to 90%), with 47% Rhodobacteraceae (gamma-Proteobacteria). beta-Proteobacteria, including Pseudoalteromonas, Alteromonas and a few vibrios, declined in the rearing period (25% on day 7 to 9% on day 15). At the end of rearing, colonisation by two members of the Burkholderiales (beta-Proteobacteria), 45% on average on day 15, had decreased overall diversity. Seawater microbiota was more stable with in all batches as one unclassified bacterium present in all batches (27 +/- 7%), 42 OTUs of alpha-Proteobacteria (19 +/- 7%) and 26 of gamma-Proteobacteria (14%). Change was due notably to a species of Cryomorphaceae (Flavobacteria) that reached 15 +/- 7% on day 15. Predatory bacteria, Bdellovivrio spp. and Bacteriovorax spp. were present (3-12%) and could participate in the regulation of bacterial populations. Bacterial assemblages in RS bioreactors remained stable and were mainly composed of ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Unknown Pacific Aquaculture 464 434 444 |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
systems ras recirculation aquaculture bacterial diversity Bivalve larvae communities dynamics hatchery marine bivalves Microbiota Pyrosequencing temperature vibrio-parahaemolyticus ACL envir geo |
spellingShingle |
systems ras recirculation aquaculture bacterial diversity Bivalve larvae communities dynamics hatchery marine bivalves Microbiota Pyrosequencing temperature vibrio-parahaemolyticus ACL envir geo Asmani, Katia Petton, Bruno Le Grand, Jacqueline Mounier, Jérôme Robert, Rene Nicolas, Jean-Louis Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
topic_facet |
systems ras recirculation aquaculture bacterial diversity Bivalve larvae communities dynamics hatchery marine bivalves Microbiota Pyrosequencing temperature vibrio-parahaemolyticus ACL envir geo |
description |
00000 ăWOS:000383370300057 International audience This study has two main objectives: (1) to implement a recycling aquaculture system (RS) for the larvae of the oyster Crassostrea gigas, and (2) to characterise the bacterial communities established in different compartments of this system. An RS with 25% fresh seawater addition per hour and another with no addition (0%) were compared with a flow-through system (FT). Larval survival was equivalent in RS and FT, but growth rate was 17% slower in RS than in FT. The physical chemical parameters remained stable, except for pH that decreased to 7.75 and salinity that increased to 37.5% in the RS 0%. In both systems, the cultivable bacteria were present in similar numbers in seawater (around 10(5) ml(-1)) and in larvae (10(3) larva(-1)) on day 15. Bacterial assemblages, characterised by 454 pyrosequencing of the V1-V3 region of 16S rRNA, were highly similar (50-65%) for compartments, regardless of rearing system and sampling time, but the compartments were clearly different from one another. At the beginning of rearing, larval microbiota was mostly composed of Proteobacteria (similar to 90%), with 47% Rhodobacteraceae (gamma-Proteobacteria). beta-Proteobacteria, including Pseudoalteromonas, Alteromonas and a few vibrios, declined in the rearing period (25% on day 7 to 9% on day 15). At the end of rearing, colonisation by two members of the Burkholderiales (beta-Proteobacteria), 45% on average on day 15, had decreased overall diversity. Seawater microbiota was more stable with in all batches as one unclassified bacterium present in all batches (27 +/- 7%), 42 OTUs of alpha-Proteobacteria (19 +/- 7%) and 26 of gamma-Proteobacteria (14%). Change was due notably to a species of Cryomorphaceae (Flavobacteria) that reached 15 +/- 7% on day 15. Predatory bacteria, Bdellovivrio spp. and Bacteriovorax spp. were present (3-12%) and could participate in the regulation of bacterial populations. Bacterial assemblages in RS bioreactors remained stable and were mainly composed of ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) Université de Brest (UBO) IFR148 ScInBioS European Project: 245119,EC:FP7:KBBE,FP7-KBBE-2009-3,REPROSEED(2010) |
format |
Article in Journal/Newspaper |
author |
Asmani, Katia Petton, Bruno Le Grand, Jacqueline Mounier, Jérôme Robert, Rene Nicolas, Jean-Louis |
author_facet |
Asmani, Katia Petton, Bruno Le Grand, Jacqueline Mounier, Jérôme Robert, Rene Nicolas, Jean-Louis |
author_sort |
Asmani, Katia |
title |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_short |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_full |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_fullStr |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Establishment of microbiota in larval culture of Pacific oyster, Crassostrea gigas |
title_sort |
establishment of microbiota in larval culture of pacific oyster, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.aquaculture.2016.07.020 https://hal.archives-ouvertes.fr/hal-01483196 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0044-8486 Aquaculture Aquaculture, Elsevier, 2016, 464, pp.434-444. ⟨10.1016/j.aquaculture.2016.07.020⟩ |
op_relation |
hal-01483196 doi:10.1016/j.aquaculture.2016.07.020 10670/1.qd75r2 https://hal.archives-ouvertes.fr/hal-01483196 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1016/j.aquaculture.2016.07.020 |
container_title |
Aquaculture |
container_volume |
464 |
container_start_page |
434 |
op_container_end_page |
444 |
_version_ |
1766394296879022080 |