Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas
Harmful algal blooms of Alexandrium spp. dinoflagellates regularly occur in French coastal waters contaminating shellfish. Studies have demonstrated that toxic Alexandrium spp. disrupt behavioural and physiological processes in marine filter-feeders, but molecular modifications triggered by phycotox...
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Online Access: | https://doi.org/10.1016/j.hal.2015.07.003 https://archimer.ifremer.fr/doc/00275/38601/38623.pdf https://archimer.ifremer.fr/doc/00275/38601/ |
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fttriple:oai:gotriple.eu:10670/1.nw18i3 2023-05-15T15:58:05+02:00 Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas Fabioux, Caroline Sulistiyani, Yeni Haberkorn, Hansy Hegaret, Helene Amzil, Zouher Soudant, Philippe https://doi.org/10.1016/j.hal.2015.07.003 https://archimer.ifremer.fr/doc/00275/38601/38623.pdf https://archimer.ifremer.fr/doc/00275/38601/ en eng Elsevier Science Bv doi:10.1016/j.hal.2015.07.003 10670/1.nw18i3 https://archimer.ifremer.fr/doc/00275/38601/38623.pdf https://archimer.ifremer.fr/doc/00275/38601/ other Archimer, archive institutionnelle de l'Ifremer Harmful Algae (1568-9883) (Elsevier Science Bv), 2015-09 , Vol. 48 , P. 55-62 envir geo Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.1016/j.hal.2015.07.003 2023-01-22T18:15:15Z Harmful algal blooms of Alexandrium spp. dinoflagellates regularly occur in French coastal waters contaminating shellfish. Studies have demonstrated that toxic Alexandrium spp. disrupt behavioural and physiological processes in marine filter-feeders, but molecular modifications triggered by phycotoxins are less well understood. This study analyzed the mRNA levels of 7 genes encoding antioxidant/detoxifying enzymes in gills of Pacific oysters (Crassostrea gigas) exposed to a cultured, toxic strain of A. minutum, a producer of paralytic shellfish toxins (PST) or fed Tisochrysis lutea (T. lutea, formerly Isochrysis sp., clone Tahitian (T. iso)), a non-toxic control diet, in four repeated experiments. Transcript levels of sigma-class glutathione S-transferase (GST), glutathione reductase (GR) and ferritin (Fer) were significantly higher in oysters exposed to A. minutum compared to oysters fed T. lutea. The detoxification pathway based upon glutathione (GSH)-conjugation of toxic compounds (phase II) is likely activated, and catalyzed by GST. This system appeared to be activated in gills probably for the detoxification of PST and/or extra-cellular compounds, produced by A. minutum. GST, GR and Fer can also contribute to antioxidant functions to prevent cellular damage from increased reactive oxygen species (ROS) originating either from A. minutum cells directly, from oyster hemocytes during immune response, or from other gill cells as by-products of detoxification. Text Crassostrea gigas Unknown Pacific Harmful Algae 48 55 62 |
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English |
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envir geo |
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envir geo Fabioux, Caroline Sulistiyani, Yeni Haberkorn, Hansy Hegaret, Helene Amzil, Zouher Soudant, Philippe Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas |
topic_facet |
envir geo |
description |
Harmful algal blooms of Alexandrium spp. dinoflagellates regularly occur in French coastal waters contaminating shellfish. Studies have demonstrated that toxic Alexandrium spp. disrupt behavioural and physiological processes in marine filter-feeders, but molecular modifications triggered by phycotoxins are less well understood. This study analyzed the mRNA levels of 7 genes encoding antioxidant/detoxifying enzymes in gills of Pacific oysters (Crassostrea gigas) exposed to a cultured, toxic strain of A. minutum, a producer of paralytic shellfish toxins (PST) or fed Tisochrysis lutea (T. lutea, formerly Isochrysis sp., clone Tahitian (T. iso)), a non-toxic control diet, in four repeated experiments. Transcript levels of sigma-class glutathione S-transferase (GST), glutathione reductase (GR) and ferritin (Fer) were significantly higher in oysters exposed to A. minutum compared to oysters fed T. lutea. The detoxification pathway based upon glutathione (GSH)-conjugation of toxic compounds (phase II) is likely activated, and catalyzed by GST. This system appeared to be activated in gills probably for the detoxification of PST and/or extra-cellular compounds, produced by A. minutum. GST, GR and Fer can also contribute to antioxidant functions to prevent cellular damage from increased reactive oxygen species (ROS) originating either from A. minutum cells directly, from oyster hemocytes during immune response, or from other gill cells as by-products of detoxification. |
format |
Text |
author |
Fabioux, Caroline Sulistiyani, Yeni Haberkorn, Hansy Hegaret, Helene Amzil, Zouher Soudant, Philippe |
author_facet |
Fabioux, Caroline Sulistiyani, Yeni Haberkorn, Hansy Hegaret, Helene Amzil, Zouher Soudant, Philippe |
author_sort |
Fabioux, Caroline |
title |
Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas |
title_short |
Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas |
title_full |
Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas |
title_fullStr |
Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas |
title_full_unstemmed |
Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas |
title_sort |
exposure to toxic alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster crassostrea gigas |
publisher |
Elsevier Science Bv |
url |
https://doi.org/10.1016/j.hal.2015.07.003 https://archimer.ifremer.fr/doc/00275/38601/38623.pdf https://archimer.ifremer.fr/doc/00275/38601/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Archimer, archive institutionnelle de l'Ifremer Harmful Algae (1568-9883) (Elsevier Science Bv), 2015-09 , Vol. 48 , P. 55-62 |
op_relation |
doi:10.1016/j.hal.2015.07.003 10670/1.nw18i3 https://archimer.ifremer.fr/doc/00275/38601/38623.pdf https://archimer.ifremer.fr/doc/00275/38601/ |
op_rights |
other |
op_doi |
https://doi.org/10.1016/j.hal.2015.07.003 |
container_title |
Harmful Algae |
container_volume |
48 |
container_start_page |
55 |
op_container_end_page |
62 |
_version_ |
1766393812647673856 |