Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts

• Published in: Journal of Chromatography A
• Main Authors: Montero, Lidia, Sánchez-Camargo, Andrea P., Garcia-Cañas, Virginia, Tanniou, Anaëlle, Stiger-Pouvreau, Valérie, Russo, Mariateresa, Rastrelli, Luca, Cifuentes, Alejandro, Herrero, Miguel, Ibáñez, Elena
• Other Authors: Instituto de Investigación en Ciencias de la Alimentación (CIAL), Universidad Autonoma de Madrid (UAM)-Consejo Superior de Investigaciones Científicas Madrid (CSIC), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Universita Mediterranea of Reggio Calabria Reggio Calabria, Università degli Studi di Salerno (UNISA), European Project: 132832,FCT::,SEAS-ERA/2012,SEAS-ERA/0001/2012(2013)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2016
• Subjects: Comprehensive LC; Brown algae; Anti-proliferative activity; ACL; Phenolic compounds; Phlorotannins; Sargassum muticum; envir; geo; Norway; North Atlantic
• Online Access: https://doi.org/10.1016/j.chroma.2015.07.053; https://hal.archives-ouvertes.fr/hal-01483241

International audience In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extreme locations of a latitudinal gradient from Portugal and Norway, were found to be the richest on total phenols and, particularly, on phlorotannins, containing up to 148.97 and 5.12 mg phloroglucinol equivalents g−1, respectively. The extracts obtained from these locations were further purified and chemically characterized using a modified HILIC × RP-DAD-MS/MS method. The application of this methodology allowed the tentative identification of a great variability of phlorotannins with different degrees of polymerization (from 3 to 11) and structures, determined for the first time in S. muticum. The most-abundant phlorotannins on these samples were fuhalols, hydroxyfuhalols and phlorethols, showing also particularities and important differences depending on the geographical location. Afterwards, the antiproliferative activity of these extracts against HT-29 adenocarcinoma colon cancer cells was studied. Results revealed that the richest S. muticum samples in terms of total phlorotannins, i.e., those from Norway, presented the highest activity, showing a good cytotoxic potential at concentrations in the medium micromolar range.