Comparative analysis of hemocyte properties from Mytilus edulis desolationis and Aulacomya ater in the Kerguelen Islands.
International audience The capability of bivalve molluscs to respond to environmental stresses largely depends upon their cellular immunity. Accordingly, shift in habitat conditions following thermal stress or exposure to pollutants may harm sensitive species differently, thereby modulating the biod...
Published in: | Marine Environmental Research |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2015
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.marenvres.2015.09.003 https://hal-riip.archives-ouvertes.fr/pasteur-01351903 |
id |
fttriple:oai:gotriple.eu:10670/1.muw454 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
Hemocytes Apoptosis Cadmium Flow cytometry Kerguelen Phagocytosis ACL envir psy |
spellingShingle |
Hemocytes Apoptosis Cadmium Flow cytometry Kerguelen Phagocytosis ACL envir psy Caza, France Betoulle, Stéphane Auffret, Michel Brousseau, Pauline Fournier, Michel St-Pierre, Yves Comparative analysis of hemocyte properties from Mytilus edulis desolationis and Aulacomya ater in the Kerguelen Islands. |
topic_facet |
Hemocytes Apoptosis Cadmium Flow cytometry Kerguelen Phagocytosis ACL envir psy |
description |
International audience The capability of bivalve molluscs to respond to environmental stresses largely depends upon their cellular immunity. Accordingly, shift in habitat conditions following thermal stress or exposure to pollutants may harm sensitive species differently, thereby modulating the biodiversity of a given ecosystem by favoring stress-tolerant species. Here, we have compared the sensitivity of hemocytes from Mytilus edulis desolationis (M. edulis desolationis) and Aulacomya ater (A. ater) to acute thermal stress and exposure to cadmium. The two subantarctic species are commonly found in the same habitat in the isolated Kerguelen archipelago. Our results showed that the phagocytic activity and viability of hemocytes from both species were equally sensitive to increasing concentrations of cadmium. However, although in vitro exposure to cadmium induced apoptosis in hemocytes of M. edulis desolationis and A. ater, flow cytometric analyses showed that the apoptotic profile of both species differed greatly when using Annexin V and YO-PRO-1 as apoptotic markers. We also found that the total hemocyte counts decreased strongly in A. ater but not in M. edulis desolationis following an acute thermal stress. Taken together, these results showed that stress responses differed significantly in hemocytes from both species. This suggests that the co-existence of both species may be at risk following exposure to pollutants and/or changes in temperature. |
author2 |
Institut Armand Frappier (INRS-IAF) Institut National de la Recherche Scientifique Québec (INRS)-Réseau International des Instituts Pasteur (RIIP) Université de Reims Champagne-Ardenne (URCA) Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO) Université Le Havre Normandie (ULH) Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) IMMUNOTOXKER IPEV Project 409 Supported by the National Science and Engineering Council of Canada (NSERC) STPGP 430539, the Agence Nationale de laRecherche (ANR), the French Polar Institute Paul Emile Victor (IPEV Project N°409 IMMUNOTOXKER), and the Terres Australes et Antarctiques Françaises (TAAF) |
format |
Article in Journal/Newspaper |
author |
Caza, France Betoulle, Stéphane Auffret, Michel Brousseau, Pauline Fournier, Michel St-Pierre, Yves |
author_facet |
Caza, France Betoulle, Stéphane Auffret, Michel Brousseau, Pauline Fournier, Michel St-Pierre, Yves |
author_sort |
Caza, France |
title |
Comparative analysis of hemocyte properties from Mytilus edulis desolationis and Aulacomya ater in the Kerguelen Islands. |
title_short |
Comparative analysis of hemocyte properties from Mytilus edulis desolationis and Aulacomya ater in the Kerguelen Islands. |
title_full |
Comparative analysis of hemocyte properties from Mytilus edulis desolationis and Aulacomya ater in the Kerguelen Islands. |
title_fullStr |
Comparative analysis of hemocyte properties from Mytilus edulis desolationis and Aulacomya ater in the Kerguelen Islands. |
title_full_unstemmed |
Comparative analysis of hemocyte properties from Mytilus edulis desolationis and Aulacomya ater in the Kerguelen Islands. |
title_sort |
comparative analysis of hemocyte properties from mytilus edulis desolationis and aulacomya ater in the kerguelen islands. |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.marenvres.2015.09.003 https://hal-riip.archives-ouvertes.fr/pasteur-01351903 |
geographic |
Kerguelen Kerguelen Islands |
geographic_facet |
Kerguelen Kerguelen Islands |
genre |
Kerguelen Islands |
genre_facet |
Kerguelen Islands |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0141-1136 EISSN: 1879-0291 Marine Environmental Research Marine Environmental Research, Elsevier science, 2015, 110, pp.174-82. ⟨10.1016/j.marenvres.2015.09.003⟩ |
op_relation |
pasteur-01351903 doi:10.1016/j.marenvres.2015.09.003 PUBMED: 26382607 10670/1.muw454 https://hal-riip.archives-ouvertes.fr/pasteur-01351903 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1016/j.marenvres.2015.09.003 |
container_title |
Marine Environmental Research |
container_volume |
110 |
container_start_page |
174 |
op_container_end_page |
182 |
_version_ |
1766055346950897664 |
spelling |
fttriple:oai:gotriple.eu:10670/1.muw454 2023-05-15T17:02:06+02:00 Comparative analysis of hemocyte properties from Mytilus edulis desolationis and Aulacomya ater in the Kerguelen Islands. Caza, France Betoulle, Stéphane Auffret, Michel Brousseau, Pauline Fournier, Michel St-Pierre, Yves Institut Armand Frappier (INRS-IAF) Institut National de la Recherche Scientifique Québec (INRS)-Réseau International des Instituts Pasteur (RIIP) Université de Reims Champagne-Ardenne (URCA) Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO) Université Le Havre Normandie (ULH) Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) IMMUNOTOXKER IPEV Project 409 Supported by the National Science and Engineering Council of Canada (NSERC) STPGP 430539, the Agence Nationale de laRecherche (ANR), the French Polar Institute Paul Emile Victor (IPEV Project N°409 IMMUNOTOXKER), and the Terres Australes et Antarctiques Françaises (TAAF) 2015-09-01 https://doi.org/10.1016/j.marenvres.2015.09.003 https://hal-riip.archives-ouvertes.fr/pasteur-01351903 en eng HAL CCSD Elsevier science pasteur-01351903 doi:10.1016/j.marenvres.2015.09.003 PUBMED: 26382607 10670/1.muw454 https://hal-riip.archives-ouvertes.fr/pasteur-01351903 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 0141-1136 EISSN: 1879-0291 Marine Environmental Research Marine Environmental Research, Elsevier science, 2015, 110, pp.174-82. ⟨10.1016/j.marenvres.2015.09.003⟩ Hemocytes Apoptosis Cadmium Flow cytometry Kerguelen Phagocytosis ACL envir psy Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2015 fttriple https://doi.org/10.1016/j.marenvres.2015.09.003 2023-01-22T16:42:26Z International audience The capability of bivalve molluscs to respond to environmental stresses largely depends upon their cellular immunity. Accordingly, shift in habitat conditions following thermal stress or exposure to pollutants may harm sensitive species differently, thereby modulating the biodiversity of a given ecosystem by favoring stress-tolerant species. Here, we have compared the sensitivity of hemocytes from Mytilus edulis desolationis (M. edulis desolationis) and Aulacomya ater (A. ater) to acute thermal stress and exposure to cadmium. The two subantarctic species are commonly found in the same habitat in the isolated Kerguelen archipelago. Our results showed that the phagocytic activity and viability of hemocytes from both species were equally sensitive to increasing concentrations of cadmium. However, although in vitro exposure to cadmium induced apoptosis in hemocytes of M. edulis desolationis and A. ater, flow cytometric analyses showed that the apoptotic profile of both species differed greatly when using Annexin V and YO-PRO-1 as apoptotic markers. We also found that the total hemocyte counts decreased strongly in A. ater but not in M. edulis desolationis following an acute thermal stress. Taken together, these results showed that stress responses differed significantly in hemocytes from both species. This suggests that the co-existence of both species may be at risk following exposure to pollutants and/or changes in temperature. Article in Journal/Newspaper Kerguelen Islands Unknown Kerguelen Kerguelen Islands Marine Environmental Research 110 174 182 |