In vivo RNA interference in oyster -vasa silencing inhibits germ cell development
International audience This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ...
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Online Access: | https://doi.org/10.1111/j.1742-4658.2009.06982.x https://hal.univ-brest.fr/hal-00663883 |
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fttriple:oai:gotriple.eu:10670/1.ml3iw0 2023-05-15T15:57:32+02:00 In vivo RNA interference in oyster -vasa silencing inhibits germ cell development Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) 2009-01-01 https://doi.org/10.1111/j.1742-4658.2009.06982.x https://hal.univ-brest.fr/hal-00663883 en eng HAL CCSD Wiley hal-00663883 doi:10.1111/j.1742-4658.2009.06982.x 10670/1.ml3iw0 https://hal.univ-brest.fr/hal-00663883 undefined Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1742-464X EISSN: 1742-4658 FEBS Journal FEBS Journal, Wiley, 2009, 9, pp.2566-2573. ⟨10.1111/j.1742-4658.2009.06982.x⟩ Crassostrea gigas germline marine bivalve RNAi vasa envir art Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2009 fttriple https://doi.org/10.1111/j.1742-4658.2009.06982.x 2023-01-22T17:15:55Z International audience This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas, particularly for mitotic proliferation and early meiosis. Our results demonstrate for the first time that in vivo RNA interference works efficiently in a bivalve species, opening major perspectives for functional genetic studies. Article in Journal/Newspaper Crassostrea gigas Unknown Vasa ENVELOPE(25.177,25.177,67.587,67.587) FEBS Journal 276 9 2566 2573 |
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fttriple |
language |
English |
topic |
Crassostrea gigas germline marine bivalve RNAi vasa envir art |
spellingShingle |
Crassostrea gigas germline marine bivalve RNAi vasa envir art Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
topic_facet |
Crassostrea gigas germline marine bivalve RNAi vasa envir art |
description |
International audience This study investigated the potential of RNA interference, which is technically challenging in bivalve mollusc species, to assess gene function in the oyster Crassostrea gigas. We designed dsRNA targeting the oyster vasa-like gene (Oyvlg), specifically expressed in oyster germ cells. In vivo injection of oyvl-dsRNA into the gonad provokes a knockdown phenotype corresponding to germ cell underproliferation and prematurely arrested meiosis througout the organ. The most severe phenotype observed is sterile. This knockdown phenotype is associated with a decrease in Oyvlg mRNA level of between 39% and 87%, and a strong reduction in OYVLG protein, to an undetectable level. Therefore, Oyvlg appears to be essential for germ cell development in Crassostrea gigas, particularly for mitotic proliferation and early meiosis. Our results demonstrate for the first time that in vivo RNA interference works efficiently in a bivalve species, opening major perspectives for functional genetic studies. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Physiologie et Ecophysiologie des Mollusques Marins (PE2M) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud |
author_facet |
Fabioux, Caroline Corporeau, Charlotte Quillien, Virgile Favrel, Pascal Huvet, Arnaud |
author_sort |
Fabioux, Caroline |
title |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_short |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_full |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_fullStr |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_full_unstemmed |
In vivo RNA interference in oyster -vasa silencing inhibits germ cell development |
title_sort |
in vivo rna interference in oyster -vasa silencing inhibits germ cell development |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://doi.org/10.1111/j.1742-4658.2009.06982.x https://hal.univ-brest.fr/hal-00663883 |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1742-464X EISSN: 1742-4658 FEBS Journal FEBS Journal, Wiley, 2009, 9, pp.2566-2573. ⟨10.1111/j.1742-4658.2009.06982.x⟩ |
op_relation |
hal-00663883 doi:10.1111/j.1742-4658.2009.06982.x 10670/1.ml3iw0 https://hal.univ-brest.fr/hal-00663883 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1111/j.1742-4658.2009.06982.x |
container_title |
FEBS Journal |
container_volume |
276 |
container_issue |
9 |
container_start_page |
2566 |
op_container_end_page |
2573 |
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1766393201092984832 |