Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy
International audience Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C. gigas may derive from the...
Published in: | Environmental Microbiology |
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Main Authors: | , , , , , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2019
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Subjects: | |
Online Access: | https://doi.org/10.1111/1462-2920.14750 https://hal.archives-ouvertes.fr/hal-02409066/file/lasa_2019.pdf https://hal.archives-ouvertes.fr/hal-02409066 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
ACL bacteria diseases diversity Temperature microbiome crassostrea-gigas envir geo |
spellingShingle |
ACL bacteria diseases diversity Temperature microbiome crassostrea-gigas envir geo Lasa, Aide Cesare, Andrea, Tassistro, Giovanni Borello, Alessio Gualdi, Stefano Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
topic_facet |
ACL bacteria diseases diversity Temperature microbiome crassostrea-gigas envir geo |
description |
International audience Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve diseases. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real-time PCR and 16SrRNA gene-based microbial profiling. A new target enrichment next-generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C. gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter. The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C. gigas. |
author2 |
Universita degli studi di Genova Universidade de Santiago de Compostela Spain (USC ) CNR Water Research Institute (IRSA) Consiglio Nazionale delle Ricerche (CNR) Universität Zürich Zürich = University of Zurich (UZH) Institute of Agrifood Research and Technology (IRTA) Marine Institute Oranmore Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Dipartimento di Scienze della Vita Trieste Università degli studi di Trieste European Project: 678589,H2020,H2020-SFS-2015-2,VIVALDI(2016) |
format |
Article in Journal/Newspaper |
author |
Lasa, Aide Cesare, Andrea, Tassistro, Giovanni Borello, Alessio Gualdi, Stefano Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi |
author_facet |
Lasa, Aide Cesare, Andrea, Tassistro, Giovanni Borello, Alessio Gualdi, Stefano Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi |
author_sort |
Lasa, Aide |
title |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_short |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_full |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_fullStr |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_full_unstemmed |
Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy |
title_sort |
dynamics of the pacific oyster pathobiota during mortality episodes in europe assessed by 16s rrna gene profiling and a new target enrichment next-generation sequencing strategy |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://doi.org/10.1111/1462-2920.14750 https://hal.archives-ouvertes.fr/hal-02409066/file/lasa_2019.pdf https://hal.archives-ouvertes.fr/hal-02409066 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2019, Pathogen and Antimicrobial Resistance Ecology, 21 (12), pp.4548-4562. ⟨10.1111/1462-2920.14750⟩ |
op_relation |
hal-02409066 doi:10.1111/1462-2920.14750 10670/1.mh62l3 https://hal.archives-ouvertes.fr/hal-02409066/file/lasa_2019.pdf https://hal.archives-ouvertes.fr/hal-02409066 |
op_doi |
https://doi.org/10.1111/1462-2920.14750 |
container_title |
Environmental Microbiology |
container_volume |
21 |
container_issue |
12 |
container_start_page |
4548 |
op_container_end_page |
4562 |
_version_ |
1766393857687158784 |
spelling |
fttriple:oai:gotriple.eu:10670/1.mh62l3 2023-05-15T15:58:08+02:00 Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next-generation sequencing strategy Lasa, Aide Cesare, Andrea, Tassistro, Giovanni Borello, Alessio Gualdi, Stefano Furones, Dolors Carrasco, Noelia Cheslett, Deborah Brechon, Amanda Paillard, Christine Bidault, Adeline Pernet, Fabrice Canesi, Laura Edomi, Paolo Pallavicini, Alberto Pruzzo, Carla Vezzulli, Luigi Universita degli studi di Genova Universidade de Santiago de Compostela Spain (USC ) CNR Water Research Institute (IRSA) Consiglio Nazionale delle Ricerche (CNR) Universität Zürich Zürich = University of Zurich (UZH) Institute of Agrifood Research and Technology (IRTA) Marine Institute Oranmore Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Dipartimento di Scienze della Vita Trieste Università degli studi di Trieste European Project: 678589,H2020,H2020-SFS-2015-2,VIVALDI(2016) 2019-12-01 https://doi.org/10.1111/1462-2920.14750 https://hal.archives-ouvertes.fr/hal-02409066/file/lasa_2019.pdf https://hal.archives-ouvertes.fr/hal-02409066 en eng HAL CCSD Society for Applied Microbiology and Wiley-Blackwell hal-02409066 doi:10.1111/1462-2920.14750 10670/1.mh62l3 https://hal.archives-ouvertes.fr/hal-02409066/file/lasa_2019.pdf https://hal.archives-ouvertes.fr/hal-02409066 Hyper Article en Ligne - Sciences de l'Homme et de la Société ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2019, Pathogen and Antimicrobial Resistance Ecology, 21 (12), pp.4548-4562. ⟨10.1111/1462-2920.14750⟩ ACL bacteria diseases diversity Temperature microbiome crassostrea-gigas envir geo Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2019 fttriple https://doi.org/10.1111/1462-2920.14750 2023-01-22T17:26:50Z International audience Infectious agents such as the bacteria Vibrio aestuarianus or Ostreid herpesvirus 1 have been repeatedly associated with dramatic disease outbreaks of Crassostrea gigas beds in Europe. Beside roles played by these pathogens, microbial infections in C. gigas may derive from the contribution of a larger number of microorganisms than previously thought, according to an emerging view supporting the polymicrobial nature of bivalve diseases. In this study, the microbial communities associated with a large number of C. gigas samples collected during recurrent mortality episodes at different European sites were investigated by real-time PCR and 16SrRNA gene-based microbial profiling. A new target enrichment next-generation sequencing protocol for selective capturing of 884 phylogenetic and virulence markers of the potential microbial pathogenic community in oyster tissue was developed allowing high taxonomic resolution analysis of the bivalve pathobiota. Comparative analysis of contrasting C. gigas samples conducted using these methods revealed that oyster experiencing mortality outbreaks displayed signs of microbiota disruption associated with the presence of previously undetected potential pathogenic microbial species mostly belonging to genus Vibrio and Arcobacter. The role of these species and their consortia should be targeted by future studies aiming to shed light on mechanisms underlying polymicrobial infections in C. gigas. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Unknown Pacific Environmental Microbiology 21 12 4548 4562 |