A species-diagnostic SNP panel for discriminating lodgepole pine, jack pine, and their interspecific hybrids
Accurate stock identification is important for forest management, yet this can be a challenge for tree species that hybridize naturally. Species discriminating molecular markers provide a means to identify stock with high accuracy. In Canada, lodgepole pine (Pinus contorta Dougl. ex Loud. var. latif...
Main Authors: | , , , |
---|---|
Format: | Other/Unknown Material |
Language: | English |
Published: |
2013
|
Subjects: | |
Online Access: | https://doi.org/10.7939/R3JW8722K https://era.library.ualberta.ca/items/24835fff-b45b-4c51-8893-3fabdeca4f5f |
id |
fttriple:oai:gotriple.eu:10670/1.luw6zz |
---|---|
record_format |
openpolar |
spelling |
fttriple:oai:gotriple.eu:10670/1.luw6zz 2023-05-15T17:46:39+02:00 A species-diagnostic SNP panel for discriminating lodgepole pine, jack pine, and their interspecific hybrids Dang, S. Cullingham, Catherine I. Cooke, Janice E. K. Coltman, David W. 2013-01-01 https://doi.org/10.7939/R3JW8722K https://era.library.ualberta.ca/items/24835fff-b45b-4c51-8893-3fabdeca4f5f en eng doi:10.7939/R3JW8722K 10670/1.luw6zz https://era.library.ualberta.ca/items/24835fff-b45b-4c51-8893-3fabdeca4f5f other ERA : Education and Research Archive envir geo Other https://vocabularies.coar-repositories.org/resource_types/c_1843/ 2013 fttriple https://doi.org/10.7939/R3JW8722K 2023-01-22T17:47:43Z Accurate stock identification is important for forest management, yet this can be a challenge for tree species that hybridize naturally. Species discriminating molecular markers provide a means to identify stock with high accuracy. In Canada, lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia) and jack pine (P. banksiana Lamb) form a large hybrid zone in Alberta and Northwest Territories; within this hybrid zone, the identification of parentals and hybrids is difficult due to an overlap in morphological characteristics. Pure and hybrid ancestry can be resolved using microsatellite markers, but these are difficult and costly to type. We have developed a panel of SNP markers using 454 transcriptome sequence data that are more cost effective, easier to score and have greater discriminating power for differentiating species than microsatellites. Our SNP panel provides accurate and cost efficient forest seed stock identification and will thereby facilitate reforestation and our pipeline can be applied to other hybrid systems globally. Other/Unknown Material Northwest Territories Unknown Canada Northwest Territories |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
fttriple |
language |
English |
topic |
envir geo |
spellingShingle |
envir geo Dang, S. Cullingham, Catherine I. Cooke, Janice E. K. Coltman, David W. A species-diagnostic SNP panel for discriminating lodgepole pine, jack pine, and their interspecific hybrids |
topic_facet |
envir geo |
description |
Accurate stock identification is important for forest management, yet this can be a challenge for tree species that hybridize naturally. Species discriminating molecular markers provide a means to identify stock with high accuracy. In Canada, lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia) and jack pine (P. banksiana Lamb) form a large hybrid zone in Alberta and Northwest Territories; within this hybrid zone, the identification of parentals and hybrids is difficult due to an overlap in morphological characteristics. Pure and hybrid ancestry can be resolved using microsatellite markers, but these are difficult and costly to type. We have developed a panel of SNP markers using 454 transcriptome sequence data that are more cost effective, easier to score and have greater discriminating power for differentiating species than microsatellites. Our SNP panel provides accurate and cost efficient forest seed stock identification and will thereby facilitate reforestation and our pipeline can be applied to other hybrid systems globally. |
format |
Other/Unknown Material |
author |
Dang, S. Cullingham, Catherine I. Cooke, Janice E. K. Coltman, David W. |
author_facet |
Dang, S. Cullingham, Catherine I. Cooke, Janice E. K. Coltman, David W. |
author_sort |
Dang, S. |
title |
A species-diagnostic SNP panel for discriminating lodgepole pine, jack pine, and their interspecific hybrids |
title_short |
A species-diagnostic SNP panel for discriminating lodgepole pine, jack pine, and their interspecific hybrids |
title_full |
A species-diagnostic SNP panel for discriminating lodgepole pine, jack pine, and their interspecific hybrids |
title_fullStr |
A species-diagnostic SNP panel for discriminating lodgepole pine, jack pine, and their interspecific hybrids |
title_full_unstemmed |
A species-diagnostic SNP panel for discriminating lodgepole pine, jack pine, and their interspecific hybrids |
title_sort |
species-diagnostic snp panel for discriminating lodgepole pine, jack pine, and their interspecific hybrids |
publishDate |
2013 |
url |
https://doi.org/10.7939/R3JW8722K https://era.library.ualberta.ca/items/24835fff-b45b-4c51-8893-3fabdeca4f5f |
geographic |
Canada Northwest Territories |
geographic_facet |
Canada Northwest Territories |
genre |
Northwest Territories |
genre_facet |
Northwest Territories |
op_source |
ERA : Education and Research Archive |
op_relation |
doi:10.7939/R3JW8722K 10670/1.luw6zz https://era.library.ualberta.ca/items/24835fff-b45b-4c51-8893-3fabdeca4f5f |
op_rights |
other |
op_doi |
https://doi.org/10.7939/R3JW8722K |
_version_ |
1766150435590111232 |