Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae
Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagi...
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fttriple:oai:gotriple.eu:10670/1.kbv0rp 2023-05-15T15:57:58+02:00 Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae Foulon, Valentin Artigaud, Sebastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guerard, Fabienne Boudry, Pierre https://doi.org/10.1038/s41598-018-33720-4 https://archimer.ifremer.fr/doc/00462/57379/59452.pdf https://archimer.ifremer.fr/doc/00462/57379/59454.pdf https://archimer.ifremer.fr/doc/00462/57379/72772.pdf https://archimer.ifremer.fr/doc/00462/57379/59453.xlsx en eng Nature Publishing Group doi:10.1038/s41598-018-33720-4 10670/1.kbv0rp https://archimer.ifremer.fr/doc/00462/57379/59452.pdf https://archimer.ifremer.fr/doc/00462/57379/59454.pdf https://archimer.ifremer.fr/doc/00462/57379/72772.pdf https://archimer.ifremer.fr/doc/00462/57379/59453.xlsx other Archimer, archive institutionnelle de l'Ifremer Scientific Reports (2045-2322) (Nature Publishing Group), 2018-10 , Vol. 8 , N. 1 , P. 15298 (14p.) geo envir Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.1038/s41598-018-33720-4 2023-01-22T16:54:56Z Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the adhesive is produced by several foot glands. This adhesive is composed of numerous fibres of differing structure, suggesting differences in chemical composition and function. Fourier transformed infrared spectroscopy indicated a mainly proteinaceous composition. Proteomic analysis of footprints was able to identify 42 proteins, among which, one uncharacterized protein was selected on the basis of its pediveliger transcriptome specificity and then located by mRNA in situ hybridization, revealing its potential role during substrate exploration before oyster larva settlement. Text Crassostrea gigas Unknown Scientific Reports 8 1 |
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geo envir Foulon, Valentin Artigaud, Sebastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guerard, Fabienne Boudry, Pierre Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
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geo envir |
description |
Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the adhesive is produced by several foot glands. This adhesive is composed of numerous fibres of differing structure, suggesting differences in chemical composition and function. Fourier transformed infrared spectroscopy indicated a mainly proteinaceous composition. Proteomic analysis of footprints was able to identify 42 proteins, among which, one uncharacterized protein was selected on the basis of its pediveliger transcriptome specificity and then located by mRNA in situ hybridization, revealing its potential role during substrate exploration before oyster larva settlement. |
format |
Text |
author |
Foulon, Valentin Artigaud, Sebastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guerard, Fabienne Boudry, Pierre |
author_facet |
Foulon, Valentin Artigaud, Sebastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guerard, Fabienne Boudry, Pierre |
author_sort |
Foulon, Valentin |
title |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_short |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_full |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_fullStr |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_full_unstemmed |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_sort |
proteinaceous secretion of bioadhesive produced during crawling and settlement of crassostrea gigas larvae |
publisher |
Nature Publishing Group |
url |
https://doi.org/10.1038/s41598-018-33720-4 https://archimer.ifremer.fr/doc/00462/57379/59452.pdf https://archimer.ifremer.fr/doc/00462/57379/59454.pdf https://archimer.ifremer.fr/doc/00462/57379/72772.pdf https://archimer.ifremer.fr/doc/00462/57379/59453.xlsx |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Archimer, archive institutionnelle de l'Ifremer Scientific Reports (2045-2322) (Nature Publishing Group), 2018-10 , Vol. 8 , N. 1 , P. 15298 (14p.) |
op_relation |
doi:10.1038/s41598-018-33720-4 10670/1.kbv0rp https://archimer.ifremer.fr/doc/00462/57379/59452.pdf https://archimer.ifremer.fr/doc/00462/57379/59454.pdf https://archimer.ifremer.fr/doc/00462/57379/72772.pdf https://archimer.ifremer.fr/doc/00462/57379/59453.xlsx |
op_rights |
other |
op_doi |
https://doi.org/10.1038/s41598-018-33720-4 |
container_title |
Scientific Reports |
container_volume |
8 |
container_issue |
1 |
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1766393690924777472 |