Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure
The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rat...
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fttriple:oai:gotriple.eu:10670/1.k7v9ow 2023-05-15T15:57:47+02:00 Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure Le Goic, Nelly Hegaret, Helene Boulais, Myrina Beguel, Jean-philippe Lambert, Christophe Fabioux, Caroline Soudant, Philippe https://doi.org/10.1002/cyto.a.22577 https://archimer.ifremer.fr/doc/00216/32760/31321.pdf https://archimer.ifremer.fr/doc/00216/32760/ en eng Wiley-blackwell doi:10.1002/cyto.a.22577 10670/1.k7v9ow https://archimer.ifremer.fr/doc/00216/32760/31321.pdf https://archimer.ifremer.fr/doc/00216/32760/ Archimer, archive institutionnelle de l'Ifremer Cytometry Part A (1552-4922) (Wiley-blackwell), 2014-12 , Vol. 85A , N. 12 , P. 1049-1056 envir socio Text https://vocabularies.coar-repositories.org/resource_types/c_18cf/ fttriple https://doi.org/10.1002/cyto.a.22577 2023-01-22T17:00:55Z The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques. Text Crassostrea gigas Pacific oyster Unknown Pacific Cytometry Part A 85 12 1049 1056 |
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envir socio Le Goic, Nelly Hegaret, Helene Boulais, Myrina Beguel, Jean-philippe Lambert, Christophe Fabioux, Caroline Soudant, Philippe Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure |
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envir socio |
description |
The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques. |
format |
Text |
author |
Le Goic, Nelly Hegaret, Helene Boulais, Myrina Beguel, Jean-philippe Lambert, Christophe Fabioux, Caroline Soudant, Philippe |
author_facet |
Le Goic, Nelly Hegaret, Helene Boulais, Myrina Beguel, Jean-philippe Lambert, Christophe Fabioux, Caroline Soudant, Philippe |
author_sort |
Le Goic, Nelly |
title |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure |
title_short |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure |
title_full |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure |
title_fullStr |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure |
title_full_unstemmed |
Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure |
title_sort |
flow cytometric assessment of morphology, viability, and production of reactive oxygen species of crassostrea gigas oocytes. application to toxic dinoflagellate (alexandrium minutum) exposure |
publisher |
Wiley-blackwell |
url |
https://doi.org/10.1002/cyto.a.22577 https://archimer.ifremer.fr/doc/00216/32760/31321.pdf https://archimer.ifremer.fr/doc/00216/32760/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Archimer, archive institutionnelle de l'Ifremer Cytometry Part A (1552-4922) (Wiley-blackwell), 2014-12 , Vol. 85A , N. 12 , P. 1049-1056 |
op_relation |
doi:10.1002/cyto.a.22577 10670/1.k7v9ow https://archimer.ifremer.fr/doc/00216/32760/31321.pdf https://archimer.ifremer.fr/doc/00216/32760/ |
op_doi |
https://doi.org/10.1002/cyto.a.22577 |
container_title |
Cytometry Part A |
container_volume |
85 |
container_issue |
12 |
container_start_page |
1049 |
op_container_end_page |
1056 |
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1766393484424511488 |