Differential Gene Expression between Fall- and Spring-Run Chinook Salmon Assessed by Long Serial Analysis of Gene Expression
Of all Pacific salmonids, Chinook salmon Oncorhynchus tshawytscha display the greatest variability in return times to freshwater. The molecular mechanisms of these differential return times have not been well described. Current methods, such as long serial analysis of gene expression (LongSAGE) and...
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fttriple:oai:gotriple.eu:10670/1.k6obwa 2023-05-15T15:32:44+02:00 Differential Gene Expression between Fall- and Spring-Run Chinook Salmon Assessed by Long Serial Analysis of Gene Expression Bernier, Jeremiah C. Birkeland, Shanda R. Cipriano, Michael J. McArthur, Andrew G. Banks, Michael A. 2008-01-01 https://doi.org/10.1577/T07-222.1 https://serval.unil.ch/notice/serval:BIB_6142E12DF155 en eng doi:10.1577/T07-222.1 10670/1.k6obwa https://serval.unil.ch/notice/serval:BIB_6142E12DF155 undefined Serveur académique Lausannois Transactions of the American Fisheries Society, vol. 137, no. 5, pp. 1378-1388 info envir Journal Article https://vocabularies.coar-repositories.org/resource_types/c_6501/ 2008 fttriple https://doi.org/10.1577/T07-222.1 2023-01-22T17:37:58Z Of all Pacific salmonids, Chinook salmon Oncorhynchus tshawytscha display the greatest variability in return times to freshwater. The molecular mechanisms of these differential return times have not been well described. Current methods, such as long serial analysis of gene expression (LongSAGE) and microarrays, allow gene expression to be analyzed for thousands of genes simultaneously. To investigate whether differential gene expression is observed between fall- and spring-run Chinook salmon from California's Central Valley, LongSAGE libraries were constructed. Three libraries containing between 25,512 and 29,372 sequenced tags (21 base pairs/tag) were generated using messenger RNA from the brains of adult Chinook salmon returning in fall and spring and from one ocean-caught Chinook salmon. Tags were annotated to genes using complementary DNA libraries from Atlantic salmon Salmo salar and rainbow trout O. mykiss. Differentially expressed genes, as estimated by differences in the number of sequence tags, were found in all pairwise comparisons of libraries (freshwater versus saltwater = 40 genes; fall versus spring = 11 genes: and spawning versus nonspawning = 51 genes). The gene for ependymin, an extracellular glycoprotein involved in behavioral plasticity in fish, exhibited the most differential expression among the three groupings. Reverse transcription polymerase chain reaction analysis verified the differential expression of ependymin between the fall- and spring-run samples. These LongSAGE libraries, the first reported for Chinook salmon, provide a window of the transcriptional changes during Chinook salmon return migration to freshwater and spawning and increase the amount of expressed sequence data. Article in Journal/Newspaper Atlantic salmon Salmo salar Unknown Pacific Transactions of the American Fisheries Society 137 5 1378 1388 |
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info envir Bernier, Jeremiah C. Birkeland, Shanda R. Cipriano, Michael J. McArthur, Andrew G. Banks, Michael A. Differential Gene Expression between Fall- and Spring-Run Chinook Salmon Assessed by Long Serial Analysis of Gene Expression |
topic_facet |
info envir |
description |
Of all Pacific salmonids, Chinook salmon Oncorhynchus tshawytscha display the greatest variability in return times to freshwater. The molecular mechanisms of these differential return times have not been well described. Current methods, such as long serial analysis of gene expression (LongSAGE) and microarrays, allow gene expression to be analyzed for thousands of genes simultaneously. To investigate whether differential gene expression is observed between fall- and spring-run Chinook salmon from California's Central Valley, LongSAGE libraries were constructed. Three libraries containing between 25,512 and 29,372 sequenced tags (21 base pairs/tag) were generated using messenger RNA from the brains of adult Chinook salmon returning in fall and spring and from one ocean-caught Chinook salmon. Tags were annotated to genes using complementary DNA libraries from Atlantic salmon Salmo salar and rainbow trout O. mykiss. Differentially expressed genes, as estimated by differences in the number of sequence tags, were found in all pairwise comparisons of libraries (freshwater versus saltwater = 40 genes; fall versus spring = 11 genes: and spawning versus nonspawning = 51 genes). The gene for ependymin, an extracellular glycoprotein involved in behavioral plasticity in fish, exhibited the most differential expression among the three groupings. Reverse transcription polymerase chain reaction analysis verified the differential expression of ependymin between the fall- and spring-run samples. These LongSAGE libraries, the first reported for Chinook salmon, provide a window of the transcriptional changes during Chinook salmon return migration to freshwater and spawning and increase the amount of expressed sequence data. |
format |
Article in Journal/Newspaper |
author |
Bernier, Jeremiah C. Birkeland, Shanda R. Cipriano, Michael J. McArthur, Andrew G. Banks, Michael A. |
author_facet |
Bernier, Jeremiah C. Birkeland, Shanda R. Cipriano, Michael J. McArthur, Andrew G. Banks, Michael A. |
author_sort |
Bernier, Jeremiah C. |
title |
Differential Gene Expression between Fall- and Spring-Run Chinook Salmon Assessed by Long Serial Analysis of Gene Expression |
title_short |
Differential Gene Expression between Fall- and Spring-Run Chinook Salmon Assessed by Long Serial Analysis of Gene Expression |
title_full |
Differential Gene Expression between Fall- and Spring-Run Chinook Salmon Assessed by Long Serial Analysis of Gene Expression |
title_fullStr |
Differential Gene Expression between Fall- and Spring-Run Chinook Salmon Assessed by Long Serial Analysis of Gene Expression |
title_full_unstemmed |
Differential Gene Expression between Fall- and Spring-Run Chinook Salmon Assessed by Long Serial Analysis of Gene Expression |
title_sort |
differential gene expression between fall- and spring-run chinook salmon assessed by long serial analysis of gene expression |
publishDate |
2008 |
url |
https://doi.org/10.1577/T07-222.1 https://serval.unil.ch/notice/serval:BIB_6142E12DF155 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Serveur académique Lausannois Transactions of the American Fisheries Society, vol. 137, no. 5, pp. 1378-1388 |
op_relation |
doi:10.1577/T07-222.1 10670/1.k6obwa https://serval.unil.ch/notice/serval:BIB_6142E12DF155 |
op_rights |
undefined |
op_doi |
https://doi.org/10.1577/T07-222.1 |
container_title |
Transactions of the American Fisheries Society |
container_volume |
137 |
container_issue |
5 |
container_start_page |
1378 |
op_container_end_page |
1388 |
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1766363218317410304 |